A5090925462- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Fetal and Pediatric Neurological Disorders
- Maternal and fetal healthcare
- Cancer, Lipids, and Metabolism
- Pregnancy and Medication Impact
- Prenatal Screening and Diagnostics
- Ovarian cancer diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- Ovarian function and disorders
- Congenital Diaphragmatic Hernia Studies
- Renal cell carcinoma treatment
- Metabolomics and Mass Spectrometry Studies
- Cardiovascular Issues in Pregnancy
- Reproductive Biology and Fertility
- Cancer, Hypoxia, and Metabolism
- Organ and Tissue Transplantation Research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
- Advanced Proteomics Techniques and Applications
- Reproductive Health and Technologies
- Cancer-related molecular mechanisms research
- Transplantation: Methods and Outcomes
Medical University of Vienna
2015-2024
Becker Technologies (Germany)
2023
Universitätszahnklinik Wien
2017-2019
Universitätsklinik für Frauenheilkunde und Geburtshilfe
2009-2018
University of Vienna
2017
Do extravillous trophoblasts (EVTs) invade non-arterial decidual vessels in healthy and pathological pregnancies?Our results reveal that trophoblast invasion of venous lymphatic is a frequent event during the first trimester pregnancy compromised recurrent spontaneous abortion (RSA). In addition, present data suggest EVTs populate regional lymph nodes pregnancy.Human remodel spiral arteries. recent report demonstrates contact veins.Tissue samples human deciduae basalis (n = 54, 6th-13th...
Endometriosis is characterized by growth of endometrial-like tissue outside the uterine cavity. Since its pathogenesis may involve epigenetic changes, we used Illumina 450K Methylation Beadchips to profile CpG methylation in endometriosis stromal cells compared from normal endometrium. We validated and extended Beadchip data using bisulfite sequencing (bis-seq), analyzed differential (DM) at CpG-level an element-level classification for groups CpGs chromatin domains. Genes found have DM...
Immune-evasion and immune checkpoints are promising new therapeutic targets for several cancer entities. In ovarian cancer, the clinical role of programmed cell death receptor ligand 1 (PD-L1) expression as mechanism to escape recognition has not been clarified yet. We analyzed PD-L1 primary peritoneal tumor tissues together with other parameters (whole transcriptomes isolated cells, local systemic cytokines metabolites) compared between recurrences. All expressed major histocompatibility...
Purpose: Cancer metabolism is characterized by alterations including aerobic glycolysis, oxidative phosphorylation, and need of fuels building blocks.Experimental Design: Targeted metabolomics preoperative follow-up sera, ascites, tumor tissues, RNA sequencing isolated cells, local systemic chemokine, immune cell infiltration data from up to 65 high-grade serous ovarian cancer patients 62 healthy controls were correlated overall survival integrated in a Systems Medicine manner.Results:...
Abstract Human extravillous trophoblast (EVT) invasion of the pregnant uterus constitutes a pivotal event for establishment maternal-fetal interface. Compromised EVT function manifesting in inadequate arterial remodeling is associated with severe pregnancy disorder early-onset preeclampsia (eoPE). Recent studies suggest that EVTs invade entire uterine vasculature including arteries, veins and lymphatics first trimester pregnancy. We therefore hypothesized EVT-derived factors accumulate...
There are known complications for fetuses after infection with SARS-CoV-2 during pregnancy. However, previous studies of in pregnancy have largely been limited to histopathologic placentas and prenatal on the effects different variants scarce date. To examine placenta fetus, we investigated fetal extra-fetal structures using MRI.For this prospective case-control study, two obstetric centers consecutively referred pregnant women MRI confirmed infection. Thirty-eight examinations were included...
ADAM12, consisting of a membrane-bound (ADAM12L) and secreted (ADAM12S) form, is expressed exclusively in regenerating developing tissue as well certain cancer types. Strong ADAM12 expression levels have been noticed the human placenta, deregulated ADAM12S were associated with various pregnancy-related disorders including pre-eclampsia intrauterine growth restriction. However, role trophoblast motility has not investigated so far. Hence, present study aimed to investigate specific function...
In high grade serous ovarian cancer patients with peritoneal involvement and unfavorable outcome would benefit from targeted therapies. The aim of this study was to find a druggable target against metastasis. We constructed planar-scale free small world-co-association gene expression network searched for clusters hub-genes associated spread. Protein impact validated via immunohistochemistry correlations deregulated pathways comprehensive omics data were used biological interpretation. A...
Bronchiolitis obliterans (BO) is a detrimental late pulmonary complication after allogeneic hematopoietic stem cell transplantation (HCT) associated with chronic graft-versus-host disease (cGvHD). When systemic immunosuppressive treatment fails to improve, severe BO patients should be considered for lung (LuTX). We present seven undergoing LuTX refractory HCT.Seven hematologic malignancies developed cGvHD involvement presenting as HCT. Evaluation was initiated failure of median 4...
Do trophoblast subtypes differ in their expression of erythroblastic leukaemia viral oncogene homologue (ERBB) receptor family members and responsiveness towards specific growth factor ligands? Our data reveal a reciprocal pattern epidermal (EGFR)/ERBB4 proliferative ERBB2/ERBB3 invasive subtypes, as well restricted to (EGF) heparin-binding (HB)-EGF. EGFR is expressed by villous cytotrophoblasts (vCTBs), but absent from extravillous trophoblasts (EVTs), which specifically up-regulate ERBB2....
High grade serous ovarian cancer (HGSOC) is among the most deadly malignancies in women, frequently involving peritoneal tumor spread. Understanding molecular mechanisms of metastasis essential to develop urgently needed targeted therapies. We described two spread types HGSOC apparent during surgery: miliary (numerous millet-sized implants) and non-miliary (few big, bulky implants). The former one defined by a more epithelial-like cell characteristic with less immune reactivity significant...
The therapeutic potential of immune checkpoint inhibitors is currently being investigated in epithelial ovarian cancer (EOC), but immunological effects the programmed cell death protein 1 (PD-1)/programmed ligand (PD-L1) axis EOC still remain poorly understood. aim this study was thus to compare infiltration rates PD-1 and PD-L1 expressing tumor infiltrating leucocytes (TILs) primary tissue metastatic intraperitoneal implants investigate its impact on overall survival (OS). Tumor specimens...
Abstract Background Gender-affirming hormone therapy has been hypothesized to reduce the patient’s reproductive potential in transmen, although exact long-term effects on future fertility are unknown. Methods In this prospective cohort study we aimed evaluate ovaries of 20 transmen by using serum levels, histomorphological analysis and fluorescence activated cells sorting (FACS) – order assess amount vital cells. Results The median total number follicles per field view was 39 (IQR 12–122)....
Do high endothelial venules (HEVs) appear in the uterus of healthy and pathological pregnancies?Our study reveals that HEVs are present non-pregnant endometrium decidua parietalis (decP) but decline upon placentation basalis (decB) less abundant decidual tissues from idiopathic, recurrent pregnancy losses (RPLs).RPL is associated with a compromised vascular phenotype.Endometrial (n = 29) first trimester 86, 6-12th week gestation) tissue samples obtained endometrial biopsies or elective...
Objective: Twin-twin transfusion syndrome (TTTS) is a severe complication of monochorionic pregnancies. Placental hydrops might be marker for TTTS. The purpose this study was to evaluate whether differences in the placental parenchyma due TTTS can seen with fetal MRI. Methods: In retrospective study, 34 pregnancies were investigated on 1.5 Tesla MR. Seventeen affected by TTTS, and 17 showed no clinical signs maturation vascular pathologies, as well extent findings allocation tissue each...
Vitrification has superseded the slow freezing method for cryopreservation of oocytes, embryos, and sperm, but there are as yet no standard protocols its use in ovarian tissue (OTC). Published diverge mainly with regard to extent supplementation dimethyl sulfoxide (DMSO) vitrification medium, an open or closed system. We investigated viability cells after vitrification/warming, using transgender patients, by means Fluorescence Activated Cells Sorting (FACS), histomorphological analyses a...
Placental anomalies visualized at midgestation by MRI are shown to be related pregnancy outcome. We performed a prospective cohort study investigate the influence of placental pathologies diagnosed with fetal on long-term neurodevelopmental outcome.In our hospital-based, cross-sectional study, all examinations pregnancies vascular pathology (i.e. infarction with/without hemorrhage, subchorionic thrombi/hemorrhages, intervillous thrombi/hemorrhages or retroplacental hematoma) between 2003 and...
Abstract Automated microscopic image analysis of immunofluorescence‐stained targets on tissue sections is challenged by autofluorescent elements such as erythrocytes, which might interfere with target segmentation and quantification. Therefore, we developed an automated system (Automated REcognition Tissue‐associated Erythrocytes; ARETE ) for in silico exclusion erythrocytes. To detect erythrocytes transmission images, a cascade boosted decision trees Haar‐like features was trained...
Abstract Introduction Epidural analgesia has been associated with intrapartum maternal fever development. Epidural-related (ERMF) is believed to be based on a non-infectious inflammatory reaction. Circulating cell-free mitochondrial deoxyribonucleic acid (mtDNA) one of the possible triggers sterile processes; however, connection not investigated so far. Therefore, this study aimed investigate mtDNA alterations in women labour ERMF comparison non-febrile women. Material and methods A total 60...