A5072669065- Renal and related cancers
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Neonatal Respiratory Health Research
- Gestational Trophoblastic Disease Studies
- Phytase and its Applications
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Chronic Myeloid Leukemia Treatments
- Melanoma and MAPK Pathways
- Genetics, Bioinformatics, and Biomedical Research
- Endometriosis Research and Treatment
- Psychiatric care and mental health services
- Photoacoustic and Ultrasonic Imaging
- Palliative Care and End-of-Life Issues
- Ultrasound Imaging and Elastography
- Optical Coherence Tomography Applications
- Cancer-related Molecular Pathways
- Enzyme Production and Characterization
- RNA modifications and cancer
- Studies on Chitinases and Chitosanases
- Proteins in Food Systems
- Colorectal Cancer Treatments and Studies
Medical University of Vienna
2020-2025
BOKU University
2019-2020
Human trophoblast stem cells (hTSCs) derived from blastocysts and first-trimester cytotrophoblasts offer an unprecedented opportunity to study the placenta. However, access human embryos placentas is limited, thus preventing establishment of hTSCs diverse genetic backgrounds associated with placental disorders. Here, we show that can be generated numerous using post-natal via two alternative methods: (1) somatic cell reprogramming adult fibroblasts OCT4, SOX2, KLF4, MYC (OSKM) (2) fate...
Abnormal placentation has been noticed in a variety of pregnancy complications such as miscarriage, early-onset preeclampsia, and fetal growth restriction. Defects the developmental program extravillous trophoblasts (EVTs), migrating from placental anchoring villi into maternal decidua its vessels, is thought to be an underlying cause. Yet, key regulatory mechanisms controlling commitment differentiation invasive trophoblast lineage remain largely elusive. Herein, comparative gene expression...
ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups from...
Failures in growth and differentiation of the early human placenta are associated with severe pregnancy disorders such as pre-eclampsia fetal restriction. However, regulatory mechanisms controlling development placental epithelial cells, trophoblasts, remain poorly elucidated. Using trophoblast stem cells (TSCs), organoids (TB-ORGs) primary cytotrophoblasts (CTBs) pregnancy, we herein show that autocrine NOTCH3 signalling controls expansion differentiation. The receptor was specifically...
3D optical coherence tomography (OCT) is used for analyses of human placenta organoids in situ without sample preparation.The trophoblast analyzed were derived from primary trophoblast. In this study a custom made ultra-high-resolution spectral domain OCT system with uniform spatial and axial resolution 2.48 μm organoid tissue was used. The obtained results align to differentiation status tested via quantitative polymerase chain reaction, Western blot analyses, immunohistochemistry,...
JEG-3 choriocarcinoma cells have been widely used as a model for placental trophoblast. Herein, 3-dimensional (3D) organoids (JEG-3-ORGs) were established using protocol that we recently developed primary cytotrophoblast (CTB-ORGs). 3D JEG-3-ORGs, cultivated in basic culture medium, rapidly divide and spontaneously undergo differentiation. Under stem cell conditions (activation of WNT/EGF signalling inhibition TGF-β signalling) smaller with reduced proliferative capacity generated...
3-dimensional trophoblast organoids (TB-ORG) represent a reliable model for studying extravillous (EVT) lineage formation and differentiation. However, restricted access to first trimester placentae requires alternative cell sources establishing placental organoids. Recently, we demonstrated EVT differentiation in JEG-3-derived Consequently, herein tested whether other commonly used trophoblastic lines, ACH-3P, HTR-8/SVneo, SWAN-71 were capable of self-organizing into subsequent Notably,...
Abstract Cereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and storage vacuoles (PSVs), the accumulation of proteins. During development, trafficking require an extensive reorganization endomembrane system. Consequently, endomembrane-modifying proteins will influence final grain quality yield. However, little known about molecular mechanism underlying system remodeling during barley development. By using...
Do high endothelial venules (HEVs) appear in the uterus of healthy and pathological pregnancies?Our study reveals that HEVs are present non-pregnant endometrium decidua parietalis (decP) but decline upon placentation basalis (decB) less abundant decidual tissues from idiopathic, recurrent pregnancy losses (RPLs).RPL is associated with a compromised vascular phenotype.Endometrial (n = 29) first trimester 86, 6-12th week gestation) tissue samples obtained endometrial biopsies or elective...
ABSTRACT Failures in growth and differentiation of the early human placenta are associated with severe pregnancy disorders such as preeclampsia fetal restriction. However, regulatory mechanisms controlling development its epithelial cells, trophoblasts, remain poorly elucidated. Using trophoblast stem cells (TSCs), organoids (TB-ORGs) primary cytotrophoblasts (CTBs) pregnancy, we herein show that autocrine NOTCH3 signalling controls placental expansion differentiation. receptor was...
Abstract ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups...
Most low-penetrance genetic risk factors for cancer are located in noncoding regions, presumably altering the regulation of neighboring genes. The poorly characterized Indel polymorphism rs150550023 (rs3730485; del1518) promoter MDM2 (human homolog mouse double minute 2) is a biologically plausible candidate factor, which might influence expression MDM2, key negative regulator central tumor suppressor p53. Here, we genotyped Central European hospital-based case-control study 407 breast...
SUMMARY Human trophoblast stem cells (hTSC) derived from blastocysts and first-trimester cytotrophoblasts offer an unprecedented opportunity to study the human placenta. However, access embryos first trimester placentas is limited thus preventing establishment of hTSC a variety genetic backgrounds associated with placental disorders. In present study, we show that can be generated numerous using post-natal via two alternative methods: (I) somatic cell reprogramming adult fibroblasts Yamanaka...
Abstract Cereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and storage vacuoles (PSVs), the accumulation of proteins. PBs can be used efficient biotechnological systems to produce high yields stable recombinant During development, trafficking require an extensive reorganization endomembrane system. Consequently, endomembrane-modifying proteins will influence final grain quality, yield production. Barley,...