A5055288567- Folate and B Vitamins Research
- Pregnancy and preeclampsia studies
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Gut microbiota and health
- Global Cancer Incidence and Screening
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Colorectal Cancer Screening and Detection
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Biomedical Text Mining and Ontologies
- Pluripotent Stem Cells Research
- Cervical Cancer and HPV Research
- Renal and related cancers
Flinders University
2022-2025
Adelaide Institute for Sleep Health
2022
Florida College
2020
University of Florida
2020
ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups from...
Folic acid (FA) food fortification in Australia has resulted a higher-than-expected intake of FA during pregnancy. High is associated with increased insulin resistance and gestational diabetes. We aimed to establish whether maternal one-carbon metabolism hormones that regulate glucose homeostasis change healthy pregnancies post-FA fortification. Circulating folate, B12, homocysteine, prolactin (PRL), human placental lactogen (hPL) growth hormone (GH2) were measured early pregnancy blood...
High yield and integrity of placental RNA are crucial for transcriptomics studies. We assessed the effects time to collection post-delivery; tissue storage, amount method used extraction; mode delivery; type on total yield. The optimal protocol extraction from includes cryofreezing sample upon 50 mg using TRIzol reagent. Decidua yielded highest quantity/mg tissue, followed by villous chorion. Comparisons with murine kidney HEK293T show lower yield, likely due highly dense heterogeneous...
Abstract Purpose: The purpose of this pilot study was to assess the differences in breast tissue microbiota composition by side (left versus right) within an individual woman and compare healthy tumor between women. We further aimed determine whether certain bacterial taxa may be associated with tumors. Methods: Bilateral samples (n=36) were collected from ten women who received routine mammoplasty procedures at University Florida Department Surgery. Archived (n=10) obtained established...