A5005388951- Ovarian cancer diagnosis and treatment
- Cancer, Lipids, and Metabolism
- Cancer Genomics and Diagnostics
- Endometrial and Cervical Cancer Treatments
- Cancer Immunotherapy and Biomarkers
- Renal cell carcinoma treatment
- Cancer, Hypoxia, and Metabolism
- PARP inhibition in cancer therapy
- Intraperitoneal and Appendiceal Malignancies
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Immune Cell Function and Interaction
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Metabolomics and Mass Spectrometry Studies
- BRCA gene mutations in cancer
- Galectins and Cancer Biology
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Ovarian function and disorders
- MicroRNA in disease regulation
- Testicular diseases and treatments
- Reproductive Biology and Fertility
Medical University of Vienna
2016-2025
Universitätsklinik für Frauenheilkunde und Geburtshilfe
2018-2025
Comprehensive Cancer Center Vienna
2015-2024
Centre Léon Bérard
2019-2020
Klinik für Frauenheilkunde
2013-2018
U-M Rogel Cancer Center
2013-2017
University of Vienna
2017
Molecular Oncology (United States)
2012-2013
Chinese Academy of Medical Sciences & Peking Union Medical College
2013
Innsbruck Medical University
2013
Circular RNAs are a recently (re-)discovered abundant RNA species with presumed function as miRNA sponges, thus part of the competing endogenous network. We analysed expression circular and linear proliferation in matched normal colon mucosa tumour tissues. predicted >1,800 proved existence five randomly chosen examples using RT-qPCR. Interestingly, ratio to isoforms was always lower compared samples even colorectal cancer cell lines. Furthermore, this correlated negatively index. The...
Objective Tumor cachexia is an important prognostic parameter in epithelial ovarian cancer (EOC). characterized by metabolic and inflammatory disturbances. These conditions might be reflected body composition measurements (BCMs) ascertained pre-operative computed tomography (CT). Thus, we aimed to identify the prognostically most relevant BCMs assessed CT EOC patients. Methods We evaluated muscle well established markers of nutritional status, as clinical-pathological parameters 140...
Gamma-glutamyltransferase (GGT) – a membrane-bound enzyme crucially involved in the cell’s detoxification pathway and apoptotic balance is tumour development, progression chemotherapy resistance. Elevated GGT serum levels are associated with increased cancer risk women worse prognosis gynaecologic cancers. The present study investigated prognostic role of ovarian patients. In this multicenter study, pre-therapeutic were ascertained 634 consecutive patients epithelial (EOC, n=567) borderline...
Immune-evasion and immune checkpoints are promising new therapeutic targets for several cancer entities. In ovarian cancer, the clinical role of programmed cell death receptor ligand 1 (PD-L1) expression as mechanism to escape recognition has not been clarified yet. We analyzed PD-L1 primary peritoneal tumor tissues together with other parameters (whole transcriptomes isolated cells, local systemic cytokines metabolites) compared between recurrences. All expressed major histocompatibility...
Abstract Background Epithelial ovarian cancer is one of the most lethal gynecologic malignancies. Clinicopathological factors do not permit precise prognosis and cannot provide guidance to specific treatments. In this study we assessed tumor infiltrating CD8+ T cells in association with Ki67 proliferation index evaluated their prognostic impact EOC samples. Methods were immunohistochemically determined on tissue microarrays including 203 primary epithelial tumors. Additionally, CD8 gene...
Most patients with epithelial ovarian cancer (EOC) are diagnosed at advanced stage and have a poor prognosis. However, small proportion of these will survive, whereas others die very quickly. Clinicopathological factors do not allow precise identification subgroups. Thus, we validated molecular subclassification as new prognostic factor in EOC. One hundred ninety-four Stage II-IV EOC were characterized by whole-genome expression profiling tumor tissues classified using published 112 gene...
Purpose: Cancer metabolism is characterized by alterations including aerobic glycolysis, oxidative phosphorylation, and need of fuels building blocks.Experimental Design: Targeted metabolomics preoperative follow-up sera, ascites, tumor tissues, RNA sequencing isolated cells, local systemic chemokine, immune cell infiltration data from up to 65 high-grade serous ovarian cancer patients 62 healthy controls were correlated overall survival integrated in a Systems Medicine manner.Results:...
High-grade serous ovarian cancer (HGSOC) is the most aggressive type of and responsible for deaths caused by gynecological cancers. Numerous candidate biomarkers were identified this disease in last decades, but not sensitive or specific enough clinical applications. Hence, new HGSOC are urgently required. This study aimed to identify markers isolating different extracellular vesicle (EV) types from ascites patients according their affinities lipid-binding proteins analyzing protein cargo....
It is still a question of debate whether neutrophils, often found in the tumor microenvironment, mediate tumor-promoting or rather tumor-inhibiting activities. The present study focuses on involvement neutrophils high grade serous ovarian cancer (HGSOC). Macroscopic features classify two types peritoneal spread HGSOC. Widespread and millet sized lesions characterize miliary type, while non-miliary metastases are larger associated with better prognosis. Multi-omics FACS data were generated...
The immune system plays a critical role in cancer progression and overall survival. Still, it is unclear if differences the response are associated with different patterns of tumor spread apparent high grade serous ovarian patients previously described by us. In this study we aimed to assess miliary (widespread, millet-sized lesions) non-miliary (bigger, exophytically growing implants) spread. To achieve comprehensively analyzed tissues, blood, ascites from 41 using immunofluorescence, flow...
// Katharina Auer 1 , Anna Bachmayr-Heyda Stefanie Aust Nyamdelger Sukhbaatar Agnes Teresa Reiner Christoph Grimm Reinhard Horvat 2 Robert Zeillinger Dietmar Pils Department of Obstetrics and Gynecology, Comprehensive Cancer Center (CCC), Medical University Vienna Ludwig Boltzmann Cluster Translational Oncology, Vienna, Austria Pathology, Correspondence to: Pils, e-mail: dietmar.pils@univie.ac.at Keywords: epithelial ovarian cancer, peritoneal tumor spread, flow cytometric analysis,...
In high grade serous ovarian cancer patients with peritoneal involvement and unfavorable outcome would benefit from targeted therapies. The aim of this study was to find a druggable target against metastasis. We constructed planar-scale free small world-co-association gene expression network searched for clusters hub-genes associated spread. Protein impact validated via immunohistochemistry correlations deregulated pathways comprehensive omics data were used biological interpretation. A...
Abstract Background The role of the tumor necrosis factor receptor associated protein 1 (TRAP1) – supposed to be involved in protection cells from apoptosis and oxidative stress has just started investigated ovarian cancer. TRAP1 been shown estrogen up-regulated α (ERα) positive cancer cells. clinical impact is not clear so far significance ERα expression as therapeutic prognostic marker still controversial. Therefore, we importance together with regard clinicopathological parameters,...
High grade serous ovarian cancer (HGSOC) is among the most deadly malignancies in women, frequently involving peritoneal tumor spread. Understanding molecular mechanisms of metastasis essential to develop urgently needed targeted therapies. We described two spread types HGSOC apparent during surgery: miliary (numerous millet-sized implants) and non-miliary (few big, bulky implants). The former one defined by a more epithelial-like cell characteristic with less immune reactivity significant...
The immune system is a key player in fighting cancer. Thus, we sought to identify molecular 'immune response signature' indicating the presence of epithelial ovarian cancer (EOC) and combine this with serum protein biomarker panel increase specificity sensitivity for earlier detection EOC. Comparing expression 32,000 genes leukocytes fraction from 44 EOC patients 19 controls, three uncorrelated shrunken centroid models were selected, comprised 7, 14, 6 genes. A second selection step using...
Venous blood sampling is one of the most common diagnostic medical procedures performed in clinical practice. It has been shown that negatively loaded words may result negative affective reactions and, consequently, an increased perception pain. We aimed to evaluate whether warnings before venous might induce unnecessary pain.We included 100 healthy participants (50 females, 50 males) who were randomized 2 study groups ("sting" vs. "beware"). Directly insertion needle, warned with either...
17β-estradiol (E2) can contribute to the progression of epithelial ovarian cancer (EOC). Although majority patients with EOC are postmenopausal woman, when de novo estrogen production in ovary has ceased, cells remain exposed estrogens synthesized locally from inactive sulfonated steroid hormone precursors-such as estrone sulfate taken up circulation via sulfatase pathway. An abundance estrogen-modifying enzymes, including estrogen-activating (STS) and estrogen-inactivating...
Cancer cell lines are good in vitro models to study molecular mechanisms underlying chemoresistance and cancer recurrence. Recent works have demonstrated that most of the available ovarian unlikely high grade serous (HGSOC), major type epithelial cancer. We aimed at establishing well characterized HGSOC lines, which can be used as optimal for research. successfully established seven from provided genomic alterations transcriptomic landscapes them. They exhibited different gene expression...