A5010286816- Advanced Proteomics Techniques and Applications
- Immune cells in cancer
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Multiple Myeloma Research and Treatments
- Peptidase Inhibition and Analysis
- Occupational and environmental lung diseases
- Neuroscience and Neuropharmacology Research
- S100 Proteins and Annexins
- Cancer Cells and Metastasis
- interferon and immune responses
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Inflammatory Bowel Disease
- Cancer Research and Treatments
- Ion channel regulation and function
- Tissue Engineering and Regenerative Medicine
- Machine Learning in Bioinformatics
- Gut microbiota and health
- Medical Imaging and Pathology Studies
- Chronic Lymphocytic Leukemia Research
- Inflammasome and immune disorders
- Ubiquitin and proteasome pathways
University of Vienna
2014-2023
Czech Academy of Sciences, Institute of Analytical Chemistry
2020
Medical University of Vienna
2007-2017
Comprehensive Cancer Center Vienna
2009-2013
University Hospital Regensburg
2009
University Clinic of Traumatology
1995-1996
The remarkable feature of Schwann cells (SCs) to transform into a repair phenotype turned the spotlight on this powerful cell type. SCs provide regenerative environment for axonal re-growth after peripheral nerve injury (PNI) and play vital role in differentiation neuroblastic tumors benign subtype neuroblastoma, tumor originating from neural crest-derived neuroblasts. Hence, understanding their mode-of-action is utmost interest new approaches medicine, but also neuroblastoma therapy....
Metabolic biomonitoring in humans is typically based on the sampling of blood, plasma or urine. Although established clinical routine, these procedures are often associated with a variety compliance issues, which impeding time-course studies. Here, we show that metabolic profiling minute amounts sweat sampled from fingertips addresses this challenge. Sweat non-invasive, robust and can be accomplished repeatedly by untrained personnel. The matrix represents rich source for phenotyping. We...
Hepatocytes are known to express a large number of characteristic proteins. Transformed and cultured hepatocytes only partially maintain functional cell differentiation characteristics, which can be assessed by proteome profiling. Here, we applied 2D-PAGE analysis in addition shotgun proteomics assess the state primary human (PHH), HepG2 Hep3B cells. Out total 1995 proteins identified cytoplasm these cells, filtered 107 for hepatocytes. A 104 those were hepatocytes, 20 HepG2, 6 Hep3B....
Endothelial cells represent major effectors in inflammation and angiogenesis, processes that drive a multitude of pathological states such as atherosclerosis cancer. Both angiogenesis are interconnected with each other the sense many pro-inflammatory proteins possess proangiogenic properties vice versa. To elucidate this interplay further, we present comparative proteome study inflammatory angiogenic activated endothelial cells. HUVEC were stimulated interleukin 1-β VEGF, respectively....
Clinical proteome analysis will almost inevitably be confronted with blood constituents. Purified plasma, serum, cell or tissue samples may easily contaminated some other constituents, affecting the final result. To recognize proteins which are potentially indicative for presence of major we purified T cells, monocytes, neutrophils, erythrocytes, platelets and plasma performed comparative profiling employing 2D-PAGE in addition to shotgun proteomics. By mass analysis, 594 different were...
B cell chronic lymphocytic leukemia (B-CLL), the most common type of in adults, is still essentially incurable despite development novel therapeutic strategies. This reflects incomplete understanding pathophysiology this disease. A comprehensive proteome analysis primary human B-CLL cells and from younger as well elderly healthy donors was performed. For comparison, line JVM-13 also included. principal component comprising 6,945 proteins separated these four groups, placing aged-matched...
Pathophysiologies of cancer-associated syndromes such as cachexia are poorly understood and no routine biomarkers have been established, yet. Using shotgun proteomics, known marker molecules including PMEL, CRP, SAA, CSPG4 were found deregulated in patients with metastatic melanoma. Targeted analysis 58 selected proteins multiple reaction monitoring was applied for independent data verification. In three patients, two which suffered from cachexia, a tissue damage signature determined,...
It is still a question of debate whether neutrophils, often found in the tumor microenvironment, mediate tumor-promoting or rather tumor-inhibiting activities. The present study focuses on involvement neutrophils high grade serous ovarian cancer (HGSOC). Macroscopic features classify two types peritoneal spread HGSOC. Widespread and millet sized lesions characterize miliary type, while non-miliary metastases are larger associated with better prognosis. Multi-omics FACS data were generated...
Inflammation is a physiological process involved in many diseases. Monitoring proteins regulatory effects may help to improve our understanding of inflammation. We have analyzed proteome alterations induced peripheral blood mononuclear cells (PBMCs) upon inflammatory activation great detail using high-resolution mass spectrometry. Moreover, the activated were treated with dexamethasone investigate their response this antiphlogistic drug. From total 6886 identified proteins, 469 significantly...
The pathogenesis of multiple myeloma (MM) is regarded as a multistep process, in which an asymptomatic stage monoclonal gammopathy undetermined significance (MGUS) precedes virtually all cases MM. Molecular events characteristic for the transition from MGUS to MM are still poorly defined. We hypothesized that fibroblast-like cells tumor microenvironment critically involved Therefore, we performed comparative proteome profiling study, analyzing primary human isolated bone marrow MM, MGUS,...
In order to systematically analyze proteins fulfilling effector functionalities during inflammation, here we present a comprehensive proteome study of inflammatory activated primary human endothelial cells and fibroblasts. Cells were stimulated with interleukin 1-β fractionated in obtain secreted, cytoplasmic nuclear protein fractions. Proteins submitted data-dependent bottom up analytical platform using QExactive orbitrap the MaxQuant software for identification label-free quantification....
Multiple Myeloma (MM) is an incurable plasma cell malignancy primarily localized within the bone marrow (BM). It develops from a premalignant stage, monoclonal gammopathy of undetermined significance (MGUS), often via intermediate smoldering MM (SMM). The mechanisms progression have not yet been fully understood, all more because patients with MGUS and SMM already carry similar initial mutations as found in cells. Over last years, increased importance has attributed to tumor microenvironment...
Abstract Background Pleural mesothelioma (PM) is an aggressive malignancy with poor prognosis . Unlike many other cancers, PM mostly characterized by inactivation of tumor suppressor genes. Its highly malignant nature in absence driving oncogene mutations indicates extrinsic supply stimulating signals cells the microenvironment (TME). Cancer-associated fibroblasts (CAFs) are abundant cell type TME and have been shown to drive progression several malignancies. The aim current study was...
Ulcerative colitis [UC] is a chronic disease with rising incidence and unclear aetiology. Deep molecular phenotyping by multiomics analyses may provide novel insights into processes characteristic features of remission states. UC pathomechanisms were assessed proteome profiling human tissue specimens, obtained from five distinct colon locations for each the 12 patients included in study. Systemic disease-associated alterations evaluated thanks to cross-sectional setting mass...
Human embryonic kidney 293 cells transiently transfected with beta 3 subunits of gamma-aminobutyric acid type A receptors from the rat exhibited a specific high affinity binding for [35S]t-butylbicyclophosphorothionate (TBPS) that could be inhibited by pentobarbital, etazolate, (+)-etomidate, alphaxalone, propofol, chlormethiazole, and Ro 5-4864. The potency these compounds inhibition [35S]TBPS was similar in membranes subunit-transfected human cerebellar membranes. In contrast to maximally...
Dendritic cells (DCs), the most potent and specialized antigen-presenting cells, play a key role in regulation of adaptive immunity. Immature DCs were generated by vitro culturing peripheral blood monocytes functionally activated with classical pathogen-associated molecular pattern lipopolysaccharide (LPS). Alternative activation resulting Th-2 polarization was induced lipid oxidation products derived from 1-palmitoyl-2-arachidoyl-sn-glycerol-3-phosphorylcholin (OxPAPC). Tolerogenic obtained...
Metastasis in melanoma is associated with poor prognosis. Early detection may thus substantially improve patient survival. Here we present a novel biomarker discovery strategy based on proteome profiling and secretome analysis of primary cells. Tumor stroma cells secrete proteins that act as powerful tumor promoters. This cell cooperativity reversible be directly accessible to therapeutic intervention. The onset these characteristic events seems precede progression. Thus, specifically...
Proteome profiling is the method of choice to identify marker proteins whose expression may be characteristic for certain diseases. The formation such results from disease-related pathophysiologic processes. In healthy individuals, peripheral blood mononuclear cells (PBMCs) circulate in a quiescent cell state monitoring potential immune-relevant events, but have competence respond quickly and efficiently an inflammatory manner any invasion pathogens. Activation these most plausibly...
Breast cancer is still the most common type of in women; an important role carcinogenesis actually attributed to cancer-associated fibroblasts. In this study, we investigated whether it possible assess functional state fibroblasts through tumor tissue proteome profiling. Tissue proteomics was performed on tumor-central, tumor-near, and tumor-distant biopsy sections from breast adenocarcinoma patients, which allowed us identify 2074 proteins. Data were interpreted referring reference profiles...
Tumors and the tumor microenvironment produce multiple growth factors that influence cancer cell behavior via various signal transduction pathways. Growth factors, like transforming factor β (TGFβ) epidermal (EGF), have been shown to induce proliferation, migration, invasion in different models. Both are frequently overexpressed will often act combination. Although both being used as rational targets clinical oncology, similarities differences of their contributions migration not fully...
Fibroblasts are mesenchymal stromal cells which occur in all tissue types. While their main function is related to ECM production and physical support, they also important players wound healing, have further been recognized be able modulate inflammatory processes support tumor growth. can display distinct phenotypes, depending on origin, as well functional state.In order contribute the proteomic characterization of fibroblasts, we isolated primary human fibroblasts from skin, lung bone...
During inflammation, proteins and lipids act in a concerted fashion, calling for combined analyses. Fibroblasts are powerful mediators of chronic inflammation. However, little is known about eicosanoid formation by human fibroblasts. The aim this study was to analyze the most relevant inflammation including fibroblasts upon inflammatory stimulation subsequent treatment with dexamethasone, antiphlogistic drug. Label-free quantification applied proteome profiling, while an in-house established...
Abstract Interpretation of proteome profiling experiments largely relies on comparative analyses. False‐positive identifications may cause fatal misinterpretation data. On the other hand, analysis also suffer from false negatives, when proteins that are actually present not detected. This circumstance be as false‐positive and was hardly considered until now. Appropriate positive controls would facilitate quality assessment experiments. Based cell biology knowledge, our aim to generate a list...