A5039792228- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Cancer, Lipids, and Metabolism
- Chromatography in Natural Products
- Sphingolipid Metabolism and Signaling
- Glycosylation and Glycoproteins Research
- Lipid metabolism and biosynthesis
- Chemical Synthesis and Analysis
- Molecular spectroscopy and chirality
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Neonatal Health and Biochemistry
- Genetics, Bioinformatics, and Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Arsenic contamination and mitigation
- Endoplasmic Reticulum Stress and Disease
- Aldose Reductase and Taurine
- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- RNA Research and Splicing
- Synthesis and bioactivity of alkaloids
- Trace Elements in Health
University of Pardubice
2019-2024
University of Vienna
2012-2024
University of Chemistry and Technology, Prague
2015
Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve survival time patients, who are often diagnosed too late at an incurable stage. Several studies report dysregulation lipid metabolism in tumor cells, suggesting that changes blood lipidome accompany growth. Here we show comprehensive mass spectrometric determination a wide range serum lipids reveals statistically significant differences between pancreatic patients and healthy controls, as...
The rapid increase in lipidomic studies has led to a collaborative effort within the community establish standards and criteria for producing, documenting, disseminating data. Creating dynamic easy-to-use checklist that condenses key information about experiments into common terminology will enhance field's consistency, comparability, repeatability. Here, we describe structure rationale of established Lipidomics Minimal Reporting Checklist transparency lipidomics research.
Summary Background The amino sulphonic acid taurine reduces oxidative endoplasmatic reticulum stress and inhibits hepatic stellate cell activation, which might lead to reduction of portal pressure in cirrhosis. Aim To assess the haemodynamic effects supplementation patients with cirrhosis varices. Methods Patients venous gradient ( HVPG ) ≥12 mm Hg were included this prospective proof concept study. Concomitant nonselective beta‐blockers therapy was not allowed. received either 4 weeks oral...
Abstract Response profiling using shotgun proteomics for establishing global metallodrug mechanisms of action in two colon carcinoma cell lines, HCT116 and SW480, has been applied evaluated with the clinically approved arsenic trioxide. Surprisingly, complete established mechanism trioxide was observed by protein regulations but not cells. Comparing basal expression lines revealed an 80 % convergence identification, significant differences, which turn seem to affect extent regulation. A...
Abstract Early detection of cancer is one the unmet needs in clinical medicine. Peripheral blood analysis a preferred method for efficient population screening, because collection well embedded practice and minimally invasive patients. Lipids are important biomolecules, variations lipid concentrations can reflect pathological disorders. Lipidomic profiling human plasma by coupling ultrahigh-performance supercritical fluid chromatography mass spectrometry investigated with aim to distinguish...
During inflammation, proteins and lipids act in a concerted fashion, calling for combined analyses. Fibroblasts are powerful mediators of chronic inflammation. However, little is known about eicosanoid formation by human fibroblasts. The aim this study was to analyze the most relevant inflammation including fibroblasts upon inflammatory stimulation subsequent treatment with dexamethasone, antiphlogistic drug. Label-free quantification applied proteome profiling, while an in-house established...
Glycosphingolipids (GSL) represent a highly heterogeneous class of lipids with many cellular functions, implicated in wide spectrum human diseases. Their isolation, detection, and comprehensive structural analysis is challenging task due to the diversity GSL molecules. In this work, subclasses are isolated from plasma using an optimized monophasic ethanol–water solvent system capable recover broad range species. Obtained deproteinized subsequently purified concentrated by C18-based...
The applicability of ultrahigh-performance supercritical fluid chromatography coupled with mass spectrometry (UHPSFC/MS) for the qualitative analysis metabolites a wide polarity range (log P: -3.89-18.95) was evaluated using representative set 78 standards belonging to nucleosides, biogenic amines, carbohydrates, amino acids, and lipids. effects gradient shape percentage water (1, 2, 5%) were investigated on Viridis BEH column. screening eight stationary phases performed columns different...