A5059130544- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Ocular Surface and Contact Lens
- Lipid Membrane Structure and Behavior
- Fatty Acid Research and Health
- Connexins and lens biology
- Adipose Tissue and Metabolism
- Advanced Proteomics Techniques and Applications
- Lipid metabolism and biosynthesis
- Sphingolipid Metabolism and Signaling
- Advanced Glycation End Products research
- Bee Products Chemical Analysis
- Advancements in Transdermal Drug Delivery
- Muscle metabolism and nutrition
- Malaria Research and Control
- Mitochondrial Function and Pathology
- Insect and Arachnid Ecology and Behavior
- Neuroscience and Neuropharmacology Research
- Insect and Pesticide Research
- Cancer, Lipids, and Metabolism
- Advanced Chemical Sensor Technologies
- Urinary Bladder and Prostate Research
- Glaucoma and retinal disorders
- Diet and metabolism studies
University of Wollongong
2015-2024
Illawarra Health and Medical Research Institute
2013-2022
Mitchell School District
2018
RMIT University
2008-2013
UNSW Sydney
2007-2010
The University of Sydney
2009
Prince of Wales Hospital
2008
University of Colorado Denver
2007
Ions formed from lipids during electrospray ionization of crude lipid extracts have been mass-selected within a quadrupole linear ion trap mass spectrometer and allowed to react with ozone vapor. Gas-phase ion−molecule reactions between unsaturated ions are found yield two primary product for each carbon−carbon double bond the molecule. The mass-to-charge ratios these chemically induced fragments diagnostic position unsaturation precursor ion. This novel analytical technique, dubbed...
We present a lipidomics analysis of human Parkinson's disease tissues. have focused on the primary visual cortex, region that is devoid pathological changes and Lewy bodies; two additional regions, amygdala anterior cingulate cortex which contain bodies at different stages but do not as severe degeneration substantia nigra.Using liquid chromatography mass spectrometry techniques for an initial screen 200 lipid species, significant in 79 sphingolipid, glycerophospholipid cholesterol species...
The position(s) of carbon−carbon double bonds within lipids can dramatically affect their structure and reactivity thus has a direct bearing on biological function. Commonly employed mass spectrometric approaches to the characterization complex lipids, however, fail localize sites unsaturation molecular cannot distinguish naturally occurring regioisomers. In recent communication [Thomas, M. C.; Mitchell, T. W.; Blanksby, S. J. Am. Chem. Soc. 2006, 128, 58−59], we have presented new technique...
Contemporary lipidomics protocols are dependent on conventional tandem mass spectrometry for lipid identification. This approach is extremely powerful determining class and identifying the number of carbons degree unsaturation any acyl-chain substituents. Such analyses however, blind to isomeric variants arising from different carbon-carbon bonding motifs within these chains including double bond position, chain branching, cyclic structures. limitation arises fact that conventional, low...
To quantify the molecular lipid composition of patient-matched tear and meibum samples compare profiles.Lipids were extracted from tears by bi-phasic methods using 10:3 tert-butyl methyl ether:methanol, washed with aqueous ammonium acetate, analyzed chip-based nanoelectrospray ionization tandem mass spectrometry. Targeted precursor ion neutral loss scans identified individual lipids quantification was obtained comparison to internal standards in each class.Two hundred thirty-six species...
The complete structural elucidation of complex lipids, including glycerophospholipids, using only mass spectrometry represents a major challenge to contemporary analytical technologies. Here, we demonstrate that product ions arising from the collision-induced dissociation (CID) [M + Na](+) adduct phospholipids can be isolated and subjected subsequent gas-phase ozonolysis - known as ozone-induced (OzID) in linear ion-trap spectrometer. resulting CID/OzID experiment yields abundant are...
Ozone-induced dissociation (OzID) exploits the gas-phase reaction between mass-selected lipid ions and ozone vapor to determine position(s) of unsaturation. In this contribution, we describe modification a tandem linear ion-trap mass spectrometer specifically for OzID analyses wherein is supplied collision cell. This instrumental configuration provides spatial separation mass-selection, ozonolysis reaction, mass-analysis steps in process thus delivers significant enhancements speed...
One of the most significant challenges in contemporary lipidomics lies separation and identification lipid isomers that differ only site(s) unsaturation or geometric configuration carbon–carbon double bonds. While analytical techniques including ion mobility spectrometry (IMS) liquid chromatography (LC) can separate isomeric lipids under appropriate conditions, conventional tandem mass cannot provide unequivocal identification. To address this challenge, we have implemented ozone-induced...
Glycerophospholipids (GPs) that differ in the relative position of two fatty acyl chains on glycerol backbone (i.e., sn-positional isomers) can have distinct physicochemical properties. The unambiguous assignment chain to an individual GP represents a significant analytical challenge. Here we describe workflow where phosphatidylcholines (PCs) are subjected ESI for characterization by combination differential mobility spectrometry and MS (DMS-MS). When infused as mixture, ions formed from...
Dietary intake of long-chain fatty acids (LCFAs) plays a causative role in insulin resistance and risk diabetes. Whereas LCFAs promote lipid accumulation resistance, diets rich medium-chain (MCFAs) have been associated with increased oxidative metabolism reduced adiposity, few deleterious effects on action. The molecular mechanisms underlying these differences between dietary fat subtypes are poorly understood. To investigate this further, we treated C2C12 myotubes various (16:0, 18:1n9,...
Ozone-induced dissociation (OzID) is a novel ion activation technology that exploits the gas-phase reaction between mass-selected ions and ozone inside mass spectrometer to assign sites of unsaturation in complex lipids. Since it was first demonstrated [Thomas et al. Anal. Chem. 2008, 80, 303], method has been widely deployed for targeted lipid structure elucidation but its application high throughput liquid chromatography-based workflows limited due relatively slow nature requisite...
The development of differentiated sexual stages (gametocytes) within human red blood cells is essential for the propagation malaria parasite, since only mature gametocytes will survive in mosquito's midgut. Hence gametocytogenesis a pre-requisite transmission disease. Physiological changes involved differentiation are still enigmatic. In particular lipid metabolism—despite being central to cellular regulation and development—is not well explored. Here profiles infected with five different...
Ozonolysis of double bonds is observed during the negative ion electrospray ionization unsaturated phospholipids under conditions that produce a corona discharge. Ionic products ozonolysis are detected and characterized by mass spectrometry, mass-to-charge ratio these species can be used to unambiguously assign bond position within fatty acid radyls. The reaction consistent with gas-phase desolvated phospholipid ions in an atmosphere rich volatilized solvent. Reactions may carried out...
Abstract We have developed a protocol suitable for high‐throughput lipidomic analysis of human brain samples. The traditional Folch extraction (using chloroform and glass–glass homogenization) was compared to method combining methyl‐ tert ‐butyl ether (MTBE) with mechanical homogenization utilizing ceramic beads. This significantly reduced sample handling time increased efficiency homogenizing. Furthermore, replacing MTBE is safer (less carcinogenic/toxic), lipids dissolving in the upper...