- Coleoptera Taxonomy and Distribution
- Forest Insect Ecology and Management
- Coleoptera: Cerambycidae studies
- Scarabaeidae Beetle Taxonomy and Biogeography
- Lepidoptera: Biology and Taxonomy
- Insect-Plant Interactions and Control
- Entomological Studies and Ecology
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Mass Spectrometry Techniques and Applications
- Hemiptera Insect Studies
- Metabolomics and Mass Spectrometry Studies
- Parasite Biology and Host Interactions
- Innovations in Medical Education
- History and Developments in Astronomy
- Orthoptera Research and Taxonomy
- Pharmaceutical Practices and Patient Outcomes
- Insect behavior and control techniques
- Insects and Parasite Interactions
- Fossil Insects in Amber
- Intensive Care Unit Cognitive Disorders
- Yersinia bacterium, plague, ectoparasites research
- Anesthesia and Sedative Agents
- Mosquito-borne diseases and control
Samford University
2016-2025
Duke Medical Center
2024
American College of Clinical Pharmacy
2023
University of Iowa
1995-2021
College of New Jersey
2021
Swansea University
2021
University of Nebraska–Lincoln
2021
State Library of Iowa
2021
Texas A&M University
2021
International Atomic Energy Agency
2021
Ions formed from lipids during electrospray ionization of crude lipid extracts have been mass-selected within a quadrupole linear ion trap mass spectrometer and allowed to react with ozone vapor. Gas-phase ion−molecule reactions between unsaturated ions are found yield two primary product for each carbon−carbon double bond the molecule. The mass-to-charge ratios these chemically induced fragments diagnostic position unsaturation precursor ion. This novel analytical technique, dubbed...
The position(s) of carbon−carbon double bonds within lipids can dramatically affect their structure and reactivity thus has a direct bearing on biological function. Commonly employed mass spectrometric approaches to the characterization complex lipids, however, fail localize sites unsaturation molecular cannot distinguish naturally occurring regioisomers. In recent communication [Thomas, M. C.; Mitchell, T. W.; Blanksby, S. J. Am. Chem. Soc. 2006, 128, 58−59], we have presented new technique...
The complete structural elucidation of complex lipids, including glycerophospholipids, using only mass spectrometry represents a major challenge to contemporary analytical technologies. Here, we demonstrate that product ions arising from the collision-induced dissociation (CID) [M + Na](+) adduct phospholipids can be isolated and subjected subsequent gas-phase ozonolysis - known as ozone-induced (OzID) in linear ion-trap spectrometer. resulting CID/OzID experiment yields abundant are...
Ozone-induced dissociation (OzID) exploits the gas-phase reaction between mass-selected lipid ions and ozone vapor to determine position(s) of unsaturation. In this contribution, we describe modification a tandem linear ion-trap mass spectrometer specifically for OzID analyses wherein is supplied collision cell. This instrumental configuration provides spatial separation mass-selection, ozonolysis reaction, mass-analysis steps in process thus delivers significant enhancements speed...
Summary Hereditary angioedema (HAE) and acquired (AAE) are rare life-threatening conditions caused by deficiency of C1 inhibitor (C1INH). Both characterized recurrent unpredictable episodes mucosal swelling involving three main areas: the skin, gastrointestinal tract larynx. Swelling in results abdominal pain vomiting, while larynx may be fatal. There limited UK data on these patients to help improve practice understand more clearly burden disease. An audit tool was designed, informed...
The cnidarian-dinoflagellate symbiosis is arguably one of the most important within marine environment in that it integral to formation coral reefs. However, regulatory processes perpetuate this remain unresolved. It essential understand these processes, if we are elucidate mechanisms support growth and resource accumulation by host, conversely, recently observed reduction and/or mortality corals response rapid environmental change. This study specifically focused on area metabolic activity...
The Asian tiger mosquito (Aedes albopictus) is an invasive species and important arbovirus vector that was introduced into the U.S. in 1980's where it continues to expand its range. Winter temperature constraint northward expansion, with potential range limits located between 0° -5°C mean cold month isotherm. Connecticut within this climatic zone therefore, Ae. albopictus monitored statewide assess northern expansion delineate populations can stably persist.Ae. females were at fixed trapping...
The results of a survey to characterize pharmacy practice in emergency department (ED) settings are reported. An electronic was sent all members the American Society Health-System Pharmacists' Emergency Medicine Connect group and College Clinical Pharmacy's Practice Research Network. Approximately 400 nontrainee practitioners were invited participate survey, which open for 30 days. Descriptive statistics used analyses. Two hundred thirty-three responses that at least partially completed...
Background: Although the FDA approved acetaminophen toxicity dosing regimen for intravenous n-acetylcysteine (NAC) is a three-bag regimen, alternate regimens have been published which are generally simpler, and decrease errors adverse effects. It not clear how pervasive alternative used in hospitals US reasons change from regimen. Objective: Characterize practice patterns treating toxicity. Methods: An pilot-tested, electronic survey containing demographic pattern questions management was...
Previous studies have shown that the human lens contains glycerophospholipids with ether linkages. These lipids differ from conventional in sn-1 substituent is attached to glycerol backbone via an 1-O-alkyl or 1-O-alk-1′-enyl rather than ester bond. The present investigation employed a combination of collision-induced dissociation (CID) and ozone-induced (OzID) unambiguously distinguish such ethers. Using these methodologies was found contain several abundant glycerophosphoethanolamines,...
Background Eastern equine encephalitis (EEE) virus (Togaviridae, Alphavirus) is a highly pathogenic mosquito-borne zoonosis that responsible for occasional outbreaks of severe disease in humans and equines, resulting high mortality neurological impairment most survivors. In the past, human northeastern U.S. have occurred intermittently with no apparent pattern; however, during last decade we witnessed recurring annual emergence where EEE activity had been historically rare, expansion into...
Twenty four epiphytic bromeliads belonging to species (Tillandsia fasciculata Swartz, T. recurvata (L.), setacea and utriculata L.) were collected in Sarasota County, Florida, October-November 1997. Macroscopic invertebrate animals extracted from each by washing water, filtering, preserving 75% ethanol. Plant sizes measured several ways, their substrate was identified. Invertebrates sorted, counted, identified as far possible the level. Two (T. fasciculata, utriculata) that impound water...
The collision-induced dissociation (CID) of a range deprotonated fatty acid standards was studied using linear ion trap mass spectrometry. Neutral losses 78, 98, and 136 Da were consistently observed for acids with five or more double bonds. Comparison the MS/MS spectra docosahexaenoic (DHA) universally (13)C-labeled DHA allowed molecular formulas these neutral to be determined as C(6)H(6), C(5)H(6)O(2), C(8)H(8)O(2). Knowledge fragmentation processes then applied identify from sea anemone,...
The redbay ambrosia beetle (RAB), Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae), is an adventive pest of Lauraceae in the southeastern U.S. This wood-boring insect vectors a lethal fungus, Raffaelea lauricola T. C. Harr., Fraedrich & Aghayeva, causal agent laurel wilt (LW) disease. vector-pathogen complex responsible for extensive mortality native Persea trees South Carolina, Georgia, and northern Florida, now poses imminent threat to avocado (Persea americana Mill.)...
Eastern equine encephalitis (EEE) virus is a highly pathogenic mosquito-borne zoonosis that responsible for outbreaks of severe disease in humans and equines, resulting high mortality or neurological impairment most survivors. In the northeastern United States, EEE maintained an enzootic cycle involving ornithophilic mosquito, Culiseta melanura (Coquillett) passerine birds freshwater swamp habitats. To evaluate role Cs. morsitans (Theobald) recent episodes activity Massachusetts, we...
Abstract Of all the superfamilies within megadiverse order C oleoptera ( I nsecta), ucujoidea ucujiformia) is arguably most problematic taxonomically. The families comprising ucujidae s.l. S ilvanidae, L aemophloeidae, P assandridae and s.s. represent a large portion of cucujoid diversity. Herein we present results rigorous molecular phylogenetic analysis using maximum‐likelihood B ayesian analyses seven genes. Representatives over half (excluding cerylonid series), as well broad sampling...
Abstract The phylogenetic relationships within the beetle family Cucujidae were investigated for first time. Fifty‐seven morphological characters used maximum parsimony and Bayesian analyses. Mitochondrial genomes generated from museum specimens through genome skimming analyses in a likelihood framework due to difficulty collecting many of species lack availability ethanol‐preserved specimens. Both molecular supported monophyly four established genera: Cucujus Fabricius, Pediacus Shuckard,...