A5085956480- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Connective Tissue Growth Factor Research
- Bone health and treatments
- Advanced Proteomics Techniques and Applications
- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Dermatological and Skeletal Disorders
- Advanced Glycation End Products research
- Sphingolipid Metabolism and Signaling
- Metabolism and Genetic Disorders
- Ion-surface interactions and analysis
- Autophagy in Disease and Therapy
- Analytical chemistry methods development
- Alcohol Consumption and Health Effects
- Circadian rhythm and melatonin
- Biochemical effects in animals
- Analytical Chemistry and Chromatography
- Cancer, Lipids, and Metabolism
- Cannabis and Cannabinoid Research
- Collagen: Extraction and Characterization
- Mosquito-borne diseases and control
- Protein Hydrolysis and Bioactive Peptides
Babraham Institute
2018-2024
Biotechnology and Biological Sciences Research Council
2022
University of Birmingham
2015-2019
University of Warwick
2012-2019
Coventry (United Kingdom)
2018-2019
University College London
2019
Royal National Orthopaedic Hospital
2019
Autophagy is a fundamental catabolic process that uses unique post-translational modification, the conjugation of ATG8 protein to phosphatidylethanolamine (PE). lipidation also occurs during non-canonical autophagy, parallel pathway involving single membranes (CASM) at endolysosomal compartments, with key functions in immunity, vision, and neurobiology. It widely assumed CASM involves same PE, but this has not been formally tested. Here, we discover all ATG8s can undergo alternative...
Abstract LIPID MAPS (LIPID Metabolites and Pathways Strategy), www.lipidmaps.org, provides a systematic standardized approach to organizing lipid structural biochemical data. Founded 20 years ago, the nomenclature classification has become accepted community standard. databases for cataloging identifying lipids at varying levels of characterization in addition numerous software tools educational resources, became an ELIXIR-UK data resource 2020. This paper describes expansion existing MAPS,...
Abstract The circadian clock regulates immune responses to microbes and affects pathogen replication, but the underlying molecular mechanisms are not well understood. Here we demonstrate that components BMAL1 REV-ERBα influence several steps in hepatitis C virus (HCV) life cycle, including particle entry into hepatocytes RNA genome replication. Genetic knock out of Bmal1 over-expression or activation REV-ERB with synthetic agonists inhibits replication HCV related flaviruses dengue Zika via...
Lipidomics increasingly describes the quantitation using mass spectrometry of all lipids present in a biological sample. As power lipidomics protocols increase, thousands lipid molecular species from multiple categories can now be profiled single experiment. Observed changes due to differences often encompass large numbers structurally-related lipids, with these being regulated by enzymes well-known metabolic pathways. datasets increase complexity, interpretation their results becomes more...
<ns3:p>Lipidomics increasingly describes the quantification using mass spectrometry of all lipids present in a biological sample. As power lipidomics protocols increase, thousands lipid molecular species from multiple categories can now be profiled single experiment. Observed changes due to differences often encompass large numbers structurally-related lipids, with these being regulated by enzymes well-known metabolic pathways. datasets increase complexity, interpretation their results...
Here, we present native liquid extraction surface analysis (LESA) mass spectrometry imaging of proteins and protein complexes from mouse brain liver tissue. Intact were detected in characteristically low charge states, indicating that the remain folded. In brain, abundant such as ubiquitin β thymosin 4 homogeneously across tissue whereas other proteins, neurogranin, localised specific anatomical regions. liver, demonstrate a complex (tetrameric hemoglobin), well fatty acid binding protein....
Octahedral anticancer platinum(<sc>iv</sc>) complexes such as <italic>trans</italic>,<italic>trans</italic>,<italic>trans</italic>-[Pt(N<sub>3</sub>)<sub>2</sub>(OH)<sub>2</sub>(pyridine)<sub>2</sub>] (<bold>1</bold>) can target peptides (and proteins) by sequence-dependent platination and radical mechanisms when activated UVA or visible light; the specific products are highly dependent on their amino acid composition of peptide.
<p>Supplementary Figure S1 shows the validation of ESTIMATE stromal and immune gene signatures mRNA expression in tumour-rich TME-rich samples.</p>
<p>Supplementary Figure S4 shows LPAR expression in PDAC cell lines and ATX-mediated pro-tumorigenic stimulation by 0082T conditioned media.</p>
<p>Supplementary Figure S5 shows RNA-seq analysis of 0082T CAFs after treatment with ATX inhibitors.</p>
<div>Abstract<p>Autotaxin (ATX), encoded by <i>ENPP2</i>, is a clinical target in pancreatic ductal adenocarcinoma (PDAC). ATX catalyzes the production of lysophosphatidic acid (LPA), an important regulator within tumor microenvironment (TME), yet protumorigenic action ATX/LPA axis PDAC remains unclear. In this study, interrogating patient samples and cell line datasets, we show that TME, rather than cancer cells, responsible for majority <i>ENPP2</i>...
<p>Supplementary Figure S2 shows the validation of 0082T CAF phenotype.</p>
<p>Supplementary Figure S6 shows LLPs expression in PDAC cancer and CAFs cells.</p>
<p>Supplementary Methods</p>
<p>Supplementary Figure S3 shows ATX antibody validation and detection in cancer CAF co-cultures.</p>
Abstract Pancreatic cancer stem cells (PCSCs) play a key role in the aggressiveness of pancreatic ductal adenocarcinomas (PDAC); however, little is known about their signaling and metabolic pathways. Here we show that PCSCs have specific common proteome lipidome modulations. displayed downregulation lactate dehydrogenase A chain, upregulation trifunctional enzyme subunit alpha. The upregulated proteins are mainly involved fatty acid (FA) elongation biosynthesis unsaturated FAs. Accordingly,...
To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process previously described to be cell autonomous. We have discovered that, Caenorhabditis elegans, neuronal heat shock factor 1 (HSF-1), conserved master regulator response (HSR), causes extensive fat remodeling peripheral tissues. These changes include decrease desaturase and acid lipase expression intestine global shift saturation plasma membrane's phospholipids....
The binding sites of two ruthenium(II) organometallic complexes the form [(η6-arene)Ru(N,N)Cl]+, where arene/N,N = biphenyl (bip)/bipyridine (bipy) for complex AH076, and (bip)/o-phenylenediamine (o-pda) AH078, on peptides angiotensin bombesin have been investigated using Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. Fragmentation was performed collisionally activated dissociation (CAD), with, in some cases, additional data being provided by electron capture (ECD)....
Collagen glycation, and in particular the formation of advanced glycation end-product (AGE) crosslinks, plays a central role ageing process many long-term complications diabetes. Glucosepane, most abundant relevant AGE crosslink, has been suggested to increase stiffness tissue reduce its solubility, although no evidence is available concerning mechanisms. We have used combination computational experimental techniques study collagen-rich with relatively simple organisation further our...
Here, we present liquid extraction surface analysis (LESA) coupled with electron-induced dissociation (EID) mass spectrometry in a Fourier-transform ion cyclotron resonance spectrometer for the of small organic pharmaceutical compounds directly from dosed tissue. First, direct infusion electrospray ionisation EID and collision-induced (CID) behaviour erlotinib, moxifloxacin, clozapine olanzapine standards were compared. spectra also compared experimental or reference electron impact spectra....
The most widely used anticancer drugs are platinum complexes, but complexes of other transition metals also show promise and may widen the spectrum activity, reduce side-effects, overcome resistance. latter include organo-iridium(iii) 'piano-stool' complexes. To understand their mechanism action, it is important to discover how they bind biomolecules binding affected by functionalisation ligands bound iridium. We have characterised, MS MS/MS techniques, unusual adducts from reactions between...
Glyoxal-derived advanced glycation end-products (AGEs) are formed in physiological systems affecting protein/peptide function and structure. These AGEs generated during aging chronic diseases such as diabetes considered arginine glycating agents. Thus, the study of glyoxal-derived lysine residues amino acid competition is addressed here using acetylated non-acetylated undecapeptides, with one residue available for glycation. Tandem mass spectrometry results from a Fourier transform ion...