A5043332371- Advanced Proteomics Techniques and Applications
- Immune cells in cancer
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Tryptophan and brain disorders
- Skin and Cellular Biology Research
- Cancer, Stress, Anesthesia, and Immune Response
- Metabolomics and Mass Spectrometry Studies
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Inflammasome and immune disorders
- Protein Degradation and Inhibitors
- Multiple Myeloma Research and Treatments
- Fibroblast Growth Factor Research
- Polysaccharides and Plant Cell Walls
- Immune Cell Function and Interaction
- Eicosanoids and Hypertension Pharmacology
- Arsenic contamination and mitigation
- Isotope Analysis in Ecology
- Metal complexes synthesis and properties
- Forensic Anthropology and Bioarchaeology Studies
- Bone and Dental Protein Studies
University of Vienna
2014-2019
Abstract Organometallic metal(arene) anticancer agents require ligand exchange for their activity and this is generally believed to confer low selectivity potential cellular targets. However, using an integrated proteomics‐based target‐response profiling approach as a potent hypothesis‐generating procedure, we found unexpected target of ruthenium(arene) pyridinecarbothioamide (plecstatin) plectin, scaffold protein cytolinker, which was validated in plectin knock‐out model vitro. Plectin...
Inflammation is a physiological process involved in many diseases. Monitoring proteins regulatory effects may help to improve our understanding of inflammation. We have analyzed proteome alterations induced peripheral blood mononuclear cells (PBMCs) upon inflammatory activation great detail using high-resolution mass spectrometry. Moreover, the activated were treated with dexamethasone investigate their response this antiphlogistic drug. From total 6886 identified proteins, 469 significantly...
In order to systematically analyze proteins fulfilling effector functionalities during inflammation, here we present a comprehensive proteome study of inflammatory activated primary human endothelial cells and fibroblasts. Cells were stimulated with interleukin 1-β fractionated in obtain secreted, cytoplasmic nuclear protein fractions. Proteins submitted data-dependent bottom up analytical platform using QExactive orbitrap the MaxQuant software for identification label-free quantification....
Multiple Myeloma (MM) is an incurable plasma cell malignancy primarily localized within the bone marrow (BM). It develops from a premalignant stage, monoclonal gammopathy of undetermined significance (MGUS), often via intermediate smoldering MM (SMM). The mechanisms progression have not yet been fully understood, all more because patients with MGUS and SMM already carry similar initial mutations as found in cells. Over last years, increased importance has attributed to tumor microenvironment...
Breast cancer is still the most common type of in women; an important role carcinogenesis actually attributed to cancer-associated fibroblasts. In this study, we investigated whether it possible assess functional state fibroblasts through tumor tissue proteome profiling. Tissue proteomics was performed on tumor-central, tumor-near, and tumor-distant biopsy sections from breast adenocarcinoma patients, which allowed us identify 2074 proteins. Data were interpreted referring reference profiles...
Abstract Response profiling using shotgun proteomics for establishing global metallodrug mechanisms of action in two colon carcinoma cell lines, HCT116 and SW480, has been applied evaluated with the clinically approved arsenic trioxide. Surprisingly, complete established mechanism trioxide was observed by protein regulations but not cells. Comparing basal expression lines revealed an 80 % convergence identification, significant differences, which turn seem to affect extent regulation. A...
The target spectrum and cellular effects of a metallo-prodrug can be separated from its activated species by time-dependent shotgun proteomics.
During inflammation, proteins and lipids act in a concerted fashion, calling for combined analyses. Fibroblasts are powerful mediators of chronic inflammation. However, little is known about eicosanoid formation by human fibroblasts. The aim this study was to analyze the most relevant inflammation including fibroblasts upon inflammatory stimulation subsequent treatment with dexamethasone, antiphlogistic drug. Label-free quantification applied proteome profiling, while an in-house established...
Anti-inflammatory effects of coffee consumption have been reported to be caused by caffeine and adenosine receptor signaling. However, contradictory observed. Many kinds chronic diseases are linked inflammation; therefore a profound understanding potential is desirable.We performed ex vivo experiments with eight individuals investigating peripheral blood mononuclear cells isolated from venous before after consumption, as well in vitro applying on cells. After inflammatory stimulation the...
Abstract Metallorganische Tumortherapeutika werden durch Ligandenaustausch aktiviert, und es wird gemeinhin angenommen, dass die resultierenden aktivierten Spezies geringe Selektivität für potenzielle zelluläre Ziele zeigen. Durch ein Proteomik‐basiertes Ziel‐Antwort‐Profiling konnte jedoch nachgewiesen werden, Ruthenium‐Aren‐Pyridincarbothioamid (Plecstatin) eine unerwartet hohe Plectin, Strukturprotein Vernetzer des Zytoskeletts, zeigt, was in einem Plectin‐Knock‐out‐Modell vitro validiert...
Classical drug assays are often confined to single molecules and targeting pathways. However, it is also desirable investigate the effects of complex mixtures on systems such as living cells including natural multitude signalling Evidence based herbal medicine has motivated us potential beneficial health Mucor racemosus (M rac) extracts. Secondary metabolites M rac were collected using a good-manufacturing process (GMP) approved production line validated manufacturing process, in order...
Metallhaltige Tumortherapeutika werden oft als Prodrugs mit absichtlich geringer Selektivität konzipiert. Dagegen beschreiben S. M. Meier, C. Gerner et al. in der Zuschrift auf 8379 die unerwartet große eines metallorganischen Ruthenium(II)-Komplexes, einer Kombination Proteomik-basierter Methoden, dem Ziel-Antwort-Profiling, erhalten wurde. Plectin wurde zentrale zelluläre Zielverbindung identifiziert. Das Plectin-Targeting wechselwirkt Mikrotubulinetzwerk und könnte daher zur...