A5082924805- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Immune cells in cancer
- Wnt/β-catenin signaling in development and cancer
- CNS Lymphoma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Cancer survivorship and care
- Cancer Immunotherapy and Biomarkers
- Innovations in Medical Education
- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Clinical practice guidelines implementation
- Extracellular vesicles in disease
- Lung Cancer Research Studies
- Immune Cell Function and Interaction
- Radiology practices and education
- Polyomavirus and related diseases
- Ferroptosis and cancer prognosis
Universitätsmedizin Göttingen
2012-2025
University Hospital Regensburg
2016-2025
Comprehensive Cancer Center Erlangen
2025
University of Regensburg
2017-2024
Fraunhofer Institute for Toxicology and Experimental Medicine
2022-2024
Regensburg University of Applied Sciences
2024
Comprehensive Cancer Center Mainfranken
2024
University Medical Center
2023
University of Göttingen
2009-2022
Bipar
2013
Abstract Tumor-associated macrophages may influence tumor progression, angiogenesis and invasion. To investigate mechanisms by which interact with cells, we developed an in vitro coculture model. Previously reported that enhanced invasiveness of the cells a TNF-α- matrix metalloprotease-dependent manner. In this report, studied intracellular signaling pathways induction inflammatory genes malignant under macrophage coculture. We report ovarian or breast cancer cell lines led to...
Interactions between neoplastic and stromal cells contribute to tumor progression. Wnt genes, involved in cell migration often deregulated cancers, are attractive candidates regulate these effects. We have recently shown that coculture of breast cancer with macrophages enhances invasiveness via matrix metalloproteases TNF-α. Here we demonstrate MCF-7 leads up-regulation 5a the latter. This was accompanied by activation AP-1/c-Jun MCF-7. Recombinant mimicked effect. also detectable...
•Glycolytic index in melanoma negatively correlates with response to anti-PD1 therapy•Blocking lactate transport or knock out of glycolytic genes improves checkpoint therapy•Diclofenac blocks the transporters MCT1 and MCT4 a COX-independent manner•Inhibition glycolysis by MCT blockade does not impede T cell function SummaryTumor-derived lactic acid inhibits natural killer (NK) and, thereby, tumor immunosurveillance. Here, we report that patients high expression glycolysis-related show worse...
Abstract Although there is increasing evidence that blood‐derived macrophages support tumor progression, it still unclear whether specialized resident macrophages, such as brain microglia, also play a prominent role in metastasis formation. Here, we show microglia enhance invasion and colonization of tissue by breast cancer cells, serving both active transporters guiding rails. This antagonized inactivation well the Wnt inhibitor Dickkopf‐2. Proinvasive demonstrate altered morphology, but...
A role of WNT signaling for primary breast cancers the basal-like subtype and as a predictor brain metastasis has been described. However, responsible ligand not identified. To further clarify this question, we comparatively investigated 22 human cancer metastases well highly invasive cell line MDA-MB-231 weakly motile MCF-7 models luminal subtype. WNT5A B were found overexpressed in cells compared with MCF-7. This corresponded to reduction invasiveness by inhibitors, whereas invasion was...
Tumor cells secrete not only a variety of soluble factors, but also extracellular vesicles that are known to support the establishment favorable tumor niche by influencing surrounding stroma cells.Here we show tumor-derived microvesicles (T-MV) directly influence enhancing their invasion in both autologous and heterologous manner.Neither respective vesicle-free supernatant nor MV from benign mammary mediate invasion.Uptake T-MV is essential for proinvasive effect.We further identify highly...
// Kerstin Menck 1,* , Florian Klemm Julia Christina Gross 2 Tobias Pukrop 1 Dirk Wenzel 3 Claudia Binder Dept of Hematology/Oncology, University Medicine, Göttingen, Germany Div. Signaling and Functional Genomics, German Cancer Research Center, Heidelberg, Max Planck Institute for Biophysical Chemistry, * These authors contributed equally to this work. Correspondence: Binder, email: Keywords : Breast cancer, exosomes, Evi, microvesicles, macrophages, Wnt 5a Received August 27, 2013...
Malignant melanoma is the deadliest of skin cancers. Melanoma frequently metastasizes to brain, resulting in dismal survival. Nevertheless, mechanisms that govern early metastatic growth and interactions disseminated cells with brain microenvironment are largely unknown. To study hallmarks niche formation, we established a transplantable model spontaneous metastasis immunocompetent mice developed molecular tools for quantitative detection micrometastases. Here demonstrate micrometastases...
Abstract The sentinel and immune functions of microglia require rapid appropriate reactions to infection damage. Their Toll‐like receptors (TLRs) sense both as threats. However, whether activated mount uniform responses or subsets conduct selective tasks is unknown. We demonstrate that murine reorganize their TLR activations postnatally this process comes with a maturation TLR4‐organized functions. Although induction MHCI for antigen presentation remains pan‐populational feature, synthesis...
A considerable percentage of rectal cancers are resistant to standard preoperative chemoradiotherapy. Because patients with a priori-resistant tumors do not benefit from multimodal treatment, understanding and overcoming this resistance remains utmost clinical importance. We recently reported overexpression the Wnt transcription factor TCF4, also known as TCF7L2, in that were 5-fluorouracil-based signaling has been associated treatment response, we aimed investigate whether TCF4 mediates...
// Laila Siam 1 , Annalen Bleckmann 2, 3 Han-Ning Chaung 2 Alexander Mohr 4 Florian Klemm Alonso Barrantes-Freer 5 Raquel Blazquez Hendrik A. Wolff 6 Lüke 7 Veit Rohde Christine Stadelmann Tobias Pukrop Department of Neurosurgery, University Medical Center, Göttingen, Germany Hematology and Oncology, Statistics, Neuroradiology, Institute Neuropathology, Radiotherapy Radiation Internal Medicine III, Hospital Regensburg, Correspondence to: Pukrop, e-mail: tobias.pukrop@ukr.de Keywords:...
Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose valuable tool understand the underlying mechanisms these diseases. We therefore present straightforward protocol for isolation human monocytes buffy coats, followed by differentiation procedure which results high macrophage yields. The technique relies mostly on commonly available lab equipment and thus provides cost time effective way obtain large quantities...
To evaluate whether tumour-derived microvesicles (T-MV), originating from the plasma membrane, represent suitable cancer biomarkers, we isolated MV peripheral blood samples of patients with locally advanced and/or metastatic solid tumours (n = 330, including 79 head & neck cancers, 74 lung 41 breast 28 colorectal cancers and 108 other forms) controls 103). Whole preparations were characterised using flow cytometry. While carrying tumour-associated proteins MUC1, EGFR EpCAM found to be...
Microglia, innate immune cells of the CNS, sense infection and damage through overlapping receptor sets. Toll‐like (TLR) 4 recognizes bacterial lipopolysaccharide (LPS) multiple injury‐associated factors. We show that its co‐receptor CD14 serves three non‐redundant functions in microglia. First, it confers an up to 100‐fold higher LPS sensitivity compared peripheral macrophages enable efficient proinflammatory cytokine induction. Second, prevents excessive responses massive challenges via...
The metastatic colonization of the brain by carcinoma cells is still barely understood, in particular when considering interactions with host tissue. comes a substantial destruction surrounding This leads to activation damage responses resident innate immune protect, repair, and organize wound healing, but may distract from tumoricidal actions. We recently demonstrated that microglia, CNS, assist cell invasion. Here we report this fatal side effect physiological response In slice coculture...
Abstract Mutations and activation of the PI3K signaling pathway in breast cancer cells have been linked to brain metastases. However, here we describe that some metastases samples protein expression components is restricted metastatic microenvironment. In contrast therapeutic effects inhibition on cells, reaction microenvironment less understood. Therefore aimed quantify activity metastasis investigate central nervous system (CNS) First, systematically metastases, performed a prospective...
Melanoma is the deadliest skin cancer due to its high rate of metastasis, frequently brain. Brain metastases are incurable; therefore, understanding melanoma brain metastasis great clinical importance. We used a mouse model spontaneous study interactions melanomas with microenvironment. find that CXCL10 upregulated in metastasis-associated astrocytes mice and humans functionally important for chemoattraction cells. Moreover, CXCR3, receptor CXCL10, brain-tropic Targeting expression CXCR3 by...