A5024643102- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Lung Cancer Research Studies
- Cancer Cells and Metastasis
- Neuroinflammation and Neurodegeneration Mechanisms
- Management of metastatic bone disease
- Chemokine receptors and signaling
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- CAR-T cell therapy research
- Advanced Radiotherapy Techniques
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Neuroendocrine Tumor Research Advances
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Caveolin-1 and cellular processes
- Immunotherapy and Immune Responses
- Radiopharmaceutical Chemistry and Applications
- Immune cells in cancer
- Cancer survivorship and care
- Metabolomics and Mass Spectrometry Studies
University Hospital Regensburg
2016-2024
Universitätsmedizin Göttingen
2015-2019
•Glycolytic index in melanoma negatively correlates with response to anti-PD1 therapy•Blocking lactate transport or knock out of glycolytic genes improves checkpoint therapy•Diclofenac blocks the transporters MCT1 and MCT4 a COX-independent manner•Inhibition glycolysis by MCT blockade does not impede T cell function SummaryTumor-derived lactic acid inhibits natural killer (NK) and, thereby, tumor immunosurveillance. Here, we report that patients high expression glycolysis-related show worse...
Malignant melanoma is the deadliest of skin cancers. Melanoma frequently metastasizes to brain, resulting in dismal survival. Nevertheless, mechanisms that govern early metastatic growth and interactions disseminated cells with brain microenvironment are largely unknown. To study hallmarks niche formation, we established a transplantable model spontaneous metastasis immunocompetent mice developed molecular tools for quantitative detection micrometastases. Here demonstrate micrometastases...
// Laila Siam 1 , Annalen Bleckmann 2, 3 Han-Ning Chaung 2 Alexander Mohr 4 Florian Klemm Alonso Barrantes-Freer 5 Raquel Blazquez Hendrik A. Wolff 6 Lüke 7 Veit Rohde Christine Stadelmann Tobias Pukrop Department of Neurosurgery, University Medical Center, Göttingen, Germany Hematology and Oncology, Statistics, Neuroradiology, Institute Neuropathology, Radiotherapy Radiation Internal Medicine III, Hospital Regensburg, Correspondence to: Pukrop, e-mail: tobias.pukrop@ukr.de Keywords:...
Abstract Mutations and activation of the PI3K signaling pathway in breast cancer cells have been linked to brain metastases. However, here we describe that some metastases samples protein expression components is restricted metastatic microenvironment. In contrast therapeutic effects inhibition on cells, reaction microenvironment less understood. Therefore aimed quantify activity metastasis investigate central nervous system (CNS) First, systematically metastases, performed a prospective...
Melanoma is the deadliest skin cancer due to its high rate of metastasis, frequently brain. Brain metastases are incurable; therefore, understanding melanoma brain metastasis great clinical importance. We used a mouse model spontaneous study interactions melanomas with microenvironment. find that CXCL10 upregulated in metastasis-associated astrocytes mice and humans functionally important for chemoattraction cells. Moreover, CXCR3, receptor CXCL10, brain-tropic Targeting expression CXCR3 by...
More than half of all brain metastases show infiltrating rather displacing growth at the macro-metastasis/organ parenchyma interface (MMPI), a finding associated with shorter survival. The lymphoid enhancer-binding factor-1 (LEF1) is an epithelial-mesenchymal transition (EMT) transcription factor that commonly overexpressed in brain-colonizing cancer cells. Here, we LEF1 vivo breast colonization model. It shortened survival, albeit without engaging EMT MMPI. By differential proteome...
Abstract Brain metastasis in breast cancer remains difficult to treat and its incidence is increasing. Therefore, the development of new therapies utmost clinical relevance. Recently, toll-like receptor (TLR) 4 was correlated with IL6 expression poor prognosis 1 215 primaries. In contrast, we demonstrated that TLR4 stimulation reduces microglia-assisted cell invasion. However, expression, prognostic value, or therapeutic potential TLR signaling brain have not been investigated. We thus...
Small cell lung cancer (SCLC) is the most aggressive entity with an extremely limited therapeutic outcome. Most patients are diagnosed at extensive stage. However, molecular mechanisms driving SCLC invasion and metastasis remain largely elusive. We used autochthonous mouse model matched samples from primary metastatic to investigate characteristics of tumor metastasis. demonstrate that liver in triggered by Angiopoietin-2 (ANG-2)/Integrin β-1-dependent pathway cells, mediated focal adhesion...
// Florian Lüke 1 , Raquel Blazquez Rezan Fahrioglu Yamaci 2 Xin Lu Benedikt Pregler 3 Stefan Hannus 4 Karin Menhart 5 Dirk Hellwig Hans-Jürgen Wester 6 Saskia Kropf 7 Daniel Heudobler Jirka Grosse Jutta Moosbauer Markus Hutterer 8 9 10 Peter Hau J. Riemenschneider 11 Michaela Bayerlová 12 Annalen Bleckmann 12, 13 Bernhard Polzer 14 Tim Beißbarth Christoph A. Klein 2, and Tobias Pukrop Department of Internal Medicine III, University Hospital Regensburg, Germany Chair Experimental Therapy...
Lipids are a ubiquitous class of structurally complex molecules involved in various biological processes. In the fast-growing field lipidomics, preanalytical issues frequently neglected. Here, we investigated stability lipid profiles murine liver, brain, lung, heart, and spleen homogenates by quantitative flow injection analysis using tandem mass spectrometry high-resolution spectrometry. Storage tissue at room temperature showed substantial alterations reflecting lipolytic action....
The development of brain metastases (BM) can significantly increase the psycho-oncological burden in cancer patients, requiring timely intervention. In addition, this aspect may negatively affect course disease and treatment outcome. However, screening for is often overlooked clinical routine. Therefore, we analyzed extent distress a patient population with BM receiving neurosurgical resection identified characteristics associated high need
Brain metastases (BM) can present a displacing or infiltrating growth pattern, independent of the primary tumor type. Previous studies have shown that cell infiltration at macro-metastasis/brain parenchyma interface (MMPI) is correlated with poor outcome. Therefore, pre-therapeutic, non-invasive detection tool for potential metastatic MMPI would be desirable to help identify patients who may benefit from more aggressive local treatment strategy. The aim this study was specific magnetic...