A5047928864- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Bioinformatics and Genomic Networks
- Immune Cell Function and Interaction
- Computational Drug Discovery Methods
- Cancer Cells and Metastasis
- Viral Infections and Vectors
- Colorectal Cancer Treatments and Studies
- Bone health and treatments
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Lymphoma Diagnosis and Treatment
- RNA Research and Splicing
- Radiopharmaceutical Chemistry and Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- Ferroptosis and cancer prognosis
Universitätsmedizin Göttingen
2014-2025
University Hospital Münster
2020-2025
University of Münster
2023-2025
Genmab (United States)
2023
Janssen (Belgium)
2023
Morpho (United States)
2023
AstraZeneca (Brazil)
2023
Bayer (United States)
2023
Centre de Recherche en Cancérologie de Marseille
2017
Centre National de la Recherche Scientifique
2017
Targeting the surface of malignant cells has evolved into a cornerstone in cancer therapy, paradigmatically introduced by success humoral immunotherapy against CD20 lymphoma. However, tumor cell susceptibility to immunochemotherapy varies, with mostly fatal outcome cases resistant disease. Here, we show that lymphoma exosomes shield target from antibody attack and exosome biogenesis is modulated lysosome-related organelle-associated ATP-binding cassette (ABC) transporter A3 (ABCA3). B-cell...
Extracellular vesicles (EVs) are membrane particles secreted from cells into all body fluids. Several EV populations exist differing in size and cellular origin. Using differential centrifugation EVs pelleting at 14,000 g ("microvesicles" (MV)) 100,000 ("exosomes") distinguishable by protein markers. Neutral sphingomyelinase (nSMase) inhibition has been shown to inhibit exosome release since used study their functional implications. How nSMases (also known as SMPD2 SMPD3) affect the basal...
Significance Viral-like nanovesicles of endosomal origin, or “exosomes,” are newly recognized vehicles signals that cells use to communicate, in various systemic diseases, including cancer. Yet the molecular mechanisms regulate biogenesis and activity exosomes remain obscure. Here, we establish oncogenic protein SRC stimulates secretion loaded with syntenin syndecans, known co-receptors for a plethora signaling adhesion molecules. phosphorylates conserved tyrosine residues syndecans their...
Tumor cells secrete not only a variety of soluble factors, but also extracellular vesicles that are known to support the establishment favorable tumor niche by influencing surrounding stroma cells.Here we show tumor-derived microvesicles (T-MV) directly influence enhancing their invasion in both autologous and heterologous manner.Neither respective vesicle-free supernatant nor MV from benign mammary mediate invasion.Uptake T-MV is essential for proinvasive effect.We further identify highly...
// Kerstin Menck 1,* , Florian Klemm Julia Christina Gross 2 Tobias Pukrop 1 Dirk Wenzel 3 Claudia Binder Dept of Hematology/Oncology, University Medicine, Göttingen, Germany Div. Signaling and Functional Genomics, German Cancer Research Center, Heidelberg, Max Planck Institute for Biophysical Chemistry, * These authors contributed equally to this work. Correspondence: Binder, email: Keywords : Breast cancer, exosomes, Evi, microvesicles, macrophages, Wnt 5a Received August 27, 2013...
Abstract Background Contemporary deep learning approaches show cutting-edge performance in a variety of complex prediction tasks. Nonetheless, the application healthcare remains limited since methods are often considered as non-interpretable black-box models. However, machine community made recent elaborations on interpretability explaining data point-specific decisions techniques. We believe that such explanations can assist need personalized precision medicine via patient-specific...
Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose valuable tool understand the underlying mechanisms these diseases. We therefore present straightforward protocol for isolation human monocytes buffy coats, followed by differentiation procedure which results high macrophage yields. The technique relies mostly on commonly available lab equipment and thus provides cost time effective way obtain large quantities...
To evaluate whether tumour-derived microvesicles (T-MV), originating from the plasma membrane, represent suitable cancer biomarkers, we isolated MV peripheral blood samples of patients with locally advanced and/or metastatic solid tumours (n = 330, including 79 head & neck cancers, 74 lung 41 breast 28 colorectal cancers and 108 other forms) controls 103). Whole preparations were characterised using flow cytometry. While carrying tumour-associated proteins MUC1, EGFR EpCAM found to be...
Abstract Immunotherapy has revolutionized the treatment of patients with non‐small cell lung cancer (NSCLC). High expression tissue PD‐L1 (tPD‐L1) is currently only approved biomarker for predicting response. However, even tPD‐L1 low (1‐49%) and absent (<1%) might benefit from immunotherapy but, to date, there no reliable biomarker, that can predict response in this particular patient subgroup. This study aimed test whether tumour‐associated extracellular vesicles (EVs) could fill gap....
The release of extracellular vesicles (EVs) including small endosomal-derived exosomes (Exos, diameter < 100 nm) and large plasma membrane-derived microvesicles (MVs, > is a fundamental cellular process that occurs in all living cells. These transport proteins, lipids nucleic acids specific for their cell origin vitro studies have highlighted importance as mediators intercellular communication. EVs been successfully isolated from various body fluids especially blood identified promising...
Breast cancer is a heterogeneous disease and has been classified into five molecular subtypes based on gene expression profiles. Signaling processes linked to different breast clinical outcomes are still poorly understood. Aberrant regulation of Wnt signaling implicated in progression. In particular Ror1/2 receptors several other members the non-canonical pathway were associated with aggressive behavior. However, signals mediated via multiple complex pathways it clinically important...
Background: Despite substantial progress made in the last decades colorectal cancer (CRC) research, new treatment approaches are still needed to improve patients’ long-term survival. To date, promising strategy target tumor angiogenesis metabolically together with a sensitization of CRC chemo- and/or radiotherapy by PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-3) inhibition has never been tested. Therefore, initial evaluation and validation newly developed compounds such as...
Abstract Background Osteoclasts are the tissue-specific macrophage population of bone and unique in their bone-resorbing activity. Hence, they fundamental for physiology health disease. However, efficient protocols isolation study primary human osteoclasts scarce. In this study, we aimed to establish a protocol, which enables differentiation functional from monocytes. Results Human monocytes were isolated through double-density gradient donor blood. Compared standard schemes polystyrene cell...
The transcriptomic classification of primary colorectal cancer (CRC) into distinct consensus molecular subtypes (CMSs) is a well‐described strategy for patient stratification. However, the nature CRC metastases remains poorly investigated. To this end, study aimed to identify and compare organotropic CMS frequencies in liver brain metastases. Compared reported CRC, from patients were CMS4‐enriched CMS3‐depleted, whereas mainly clustered as CMS3 rarely CMS4. Regarding overall survival rates,...
Abstract T-cell large granular lymphocytic leukemia (T-LGLL) is a rare hematologic neoplasm characterized by clonal expansion of CD3 + cytotoxic T lymphocytes and highly heterogeneous clinical course. Conventional therapy primarily includes immunosuppressive regimen. However, optimal front-line approaches still need to be defined refractory disease remains challenge. Thus, we here aimed explore functional dependencies T-LGLL as basis for personalized therapeutic strategies. We performed...
// Eva Rietkötter 1 , Kerstin Menck Annalen Bleckmann 1,2 Katja Farhat 3 Meike Schaffrinski Matthias Schulz Uwe-Karsten Hanisch 4 Claudia Binder Tobias Pukrop Department of Hematology/Oncology, University Medical Center, 37099 Göttingen, Germany 2 Statistics, Cardiovascular Physiology, Institute Neuropathology, Correspondence: Pukrop, email: Keywords : Zoledronic acid, macrophages, microglia, metastasis, tumor microenvironment Received July 23, 2013 Accepted August 17, Published 19, Abstract...
// Eva Rietkötter 1 , Annalen Bleckmann Michaela Bayerlová 2 Kerstin Menck Han-Ning Chuang Britta Wenske Hila Schwartz 3 Neta Erez Claudia Binder Uwe-Karsten Hanisch 4 Tobias Pukrop 1, 5 Department of Hematology and Medical Oncology, University Center, 37075 Göttingen, Germany Statistics, Pathology, Sackler School Medicine, 69978 Tel Aviv University, Israel Institute Neuropathology, Clinic Regensburg, 93053 Correspondence to: Pukrop, e-mail: tobias.pukrop@ukr.de Keywords: anti-CSF-1,...
Breast cancer has been associated with activation of the WNT signaling pathway, although no driver mutations in genes have found yet. Instead, a high expression alternative receptor ROR2 was observed, particular breast brain metastases. However, its respective ligand and downstream this context remained unknown. We modulated human cells characterized their gene protein by RNA-Seq, qRT-PCR, immunoblots reverse phase array (RPPA) combined network analyses to understand molecular basis cancer....
Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose valuable tool understand the underlying mechanisms these diseases. We therefore present straightforward protocol for isolation human monocytes buffy coats, followed by differentiation procedure which results high macrophage yields. The technique relies mostly on commonly available lab equipment and thus provides cost time effective way obtain large quantities...