A5002050095- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- MicroRNA in disease regulation
- Bone fractures and treatments
- Connective tissue disorders research
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Proteoglycans and glycosaminoglycans research
- Orthopaedic implants and arthroplasty
- Cell Adhesion Molecules Research
- Spine and Intervertebral Disc Pathology
- Bone and Dental Protein Studies
- Circular RNAs in diseases
- Bone health and osteoporosis research
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Ultrasound in Clinical Applications
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Medical Imaging and Analysis
- S100 Proteins and Annexins
- Corneal surgery and disorders
- Regulation of Appetite and Obesity
University Hospital Münster
2011-2024
University of Münster
2018-2024
Klinik und Poliklinik für Psychosomatik und Psychotherapie
2012
Center For Reconstructive Urethral Surgery
2011
Ludwig-Maximilians-Universität München
2006-2007
Abstract Developmental osteogenesis, physiological bone remodelling and fracture healing require removal of matrix cellular debris. Osteoclasts generated by the fusion circulating monocytes degrade bone, whereas identity cells responsible for cartilage resorption is a long-standing controversial question. Here we show that degradation chondrocyte phagocytosis are mediated fatty acid binding protein 5-expressing representing septoclasts, which have mesenchymal origin not derived from...
Abstract Bone regeneration requires a well-orchestrated cellular and molecular response including robust vascularization recruitment of mesenchymal osteogenic cells. In femoral fractures, angiogenesis osteogenesis are closely coupled during the complex healing process. Here, we show with advanced longitudinal intravital multiphoton microscopy that early vascular sprouting is not directly to osteoprogenitor invasion calvarial bone regeneration. Early osteoprogenitors emerging from periosteum...
Abstract Background Osteoclasts are the tissue-specific macrophage population of bone and unique in their bone-resorbing activity. Hence, they fundamental for physiology health disease. However, efficient protocols isolation study primary human osteoclasts scarce. In this study, we aimed to establish a protocol, which enables differentiation functional from monocytes. Results Human monocytes were isolated through double-density gradient donor blood. Compared standard schemes polystyrene cell...
Abstract Background Alpha-melanocyte-stimulating-hormone (α-MSH) has been identified as a new anti-inflammatory treatment compound in rheumatoid arthritis (RA) and other inflammatory diseases. However, its direct effect on bone cell differentiation or regeneration, which is an process, too, not investigated, yet. Bone tissue significantly affected joint Additionally, signaling essential -in regeneration during fracture healing. Therefore, we evaluated the impact of α-MSH-treatment forming...
This study evaluates four compression testing methods to determine the most reliable and reproducible technique for assessing strength of murine lumbar vertebral bodies. Twenty female C57BL/6 mice (12 weeks old) were randomized into groups: Group 1, complete body (LVB) with dorsal spinal processes; 2, at surface; 3, surface after arch resection; 4, resection straightening intervertebral joint surface. A mono-axial static machine applied compression, measuring load failure, stiffness, yield...
Abstract Objective Syndecan 4, a heparan sulfate proteoglycan, has been associated with osteoarthritis. The present study was undertaken to analyze the functional role of syndecan 4 in endochondral ossification mouse embryos and adult fracture repair, which, like osteoarthritis, involves an inflammatory component. Methods Sdc4 promoter activity analyzed −/− lacZ‐knockin mice, using β‐galactosidase staining. Endochondral from embryonic day 16.5 assessed by histologic immunohistologic Bone...
The overexpression of tumor necrosis factor (TNF)-α leads to systemic as well local loss bone and cartilage is also an important regulator during fracture healing. In this study, we investigate how TNF-α inhibition using a targeted monoclonal antibody affects healing in driven animal model human rheumatoid arthritis (RA) elucidate the question whether enduring anti therapy after trauma beneficial or not.A standardized femur was applied wild type transgenic mice (hTNFtg mice), which develop...
The Gram-negative bacterium Vibrio harveyi produces and responds to three autoinducers, AI-1, AI-2, CAI-1 regulate cell density dependent gene expression by a process referred as quorum sensing. concentration of the autoinducers is sensed cognate hybrid sensor kinases, information channeled via HPt protein LuxU response regulator LuxO. Here, detailed biochemical study on enzymatic activities membrane-integrated kinase LuxN, for N-(d-3-hydroxybutanoyl)homoserine lactone (AI-1), provided. LuxN...
Abstract Bone loss is one of the consequences aging, leading to diseases such as osteoporosis and increased susceptibility fragility fractures therefore considerable morbidity mortality in humans. Here, we identify microRNA‐146a (miR‐146a) an essential epigenetic switch controlling bone with age. Mice deficient miR‐146a show regular development their skeleton. However, while WT mice start lose age, animals continue accrue throughout life span. Increased mass due generation activity...
The heparan sulfate proteoglycan Syndecan-1, a mediator of signals between the extracellular matrix and cells involved is able to interact with OPG, one major regulators osteoclastogenesis. potential osteoblasts induce osteoclastogenesis characterized by switch OPG (low osteoclastogenic potential) towards RANKL production (high potential). In present study, we investigated influence endogenous Syndecan-1 on local bone-cell-communication via RANKL/OPG-axis in murine osteoclasts wild type...
Hydropathy profile analyses of the amino acid sequence quorum-sensing hybrid histidine kinase LuxN Vibrio harveyi predict a periplasmic location N terminus. To test this, two-hybrid proteins consisting and an N-terminally fused maltose-binding protein with or without leader were analyzed regard to enzymatic activities LuxN, protease accessibility, complementation Escherichia coli malE mutant. The results strongly support terminus, implying that is anchored nine transmembrane domains in...
Abstract Enhanced osteoclast formation and function is a fundamental cause of alterations to bone structure plays an important role in several diseases impairing quality. Recent work revealed that TRP calcium channels 3 6 might play special this context. By analyzing the phenotype TRPC6-deficient mice we detected regulatory effect TRPC3 on function. These exhibit significant decrease volume per tissue volume, trabecular thickness -number together with increased number osteoclasts found...
Over the last years, murine in vivo magnetic resonance imaging (MRI) contributed to a new understanding of tissue composition, regeneration and diseases. Due artefacts generated by currently used metal implants, MRI is limited fracture healing research so far. In this study, we investigated novel MRI-compatible, ceramic intramedullary implant during bone mice. Three-point-bending revealed higher stiffness material compared implants. Electron microscopy displayed rough surface that was...
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Introduction: Neoangiogenesis drives the replacement of mineralized cartilage by trabecular bone during growth regulated molecules like e.g. VEGF, OPG and RANKL. The Heparan sulfate proteoglycan Syndecan-1 (Sdc1) while interacting with VEGF OPG, plays a role in communication osteoclasts osteoblasts development blood vessels. To understand function Sdc1 enchondral ossification we analysed structure vessel fracture healing mice deficient Sdc1.
<title>Abstract</title> Numerous genetically modified mouse models are available to evaluate gene functions related bone metabolism regulation. This study aims four prevalent biomechanical testing methods for assessing the structure and stability of murine lumbar vertebral bodies, identifying most reliable, valid, reproducible technique. The fourth vertebrae 20 female C57BL/6 mice (wild type, 12 weeks) were tested, randomized into groups: Method 1 - compression complete body (LVB) including...
Introduction Cartilage comprises chondrocytes and extracellular matrix. The matrix contains different collagens, proteoglycans, growth factors produced by chondroprogenitor cells that differentiate from proliferating to hypertrophic chondrocytes. In vitro chondrocyte is challenging due differences in behaviour between 2D 3D cultures. Our aim establish a murine spheroid culture method using study the complex interaction of on chondro-osseous border during enchondral ossification. Method...
<h3>Background</h3> Micro RNAs (miRNAs) play a crucial role in the regulation of bone metabolism. MiR-146a, an important anti-inflammatory miRNA, was found to negatively impact osteogenesis and regeneration <i>in vitro</i>, by controlling differentiation mesenchymal stem cells. But date miR-146a remodelling, its influence on stability development osteoporosis is not known. <h3>Objectives</h3> The objective this project analysis <h3>Methods</h3> Systemic bone, tibiae femur, wt deficient...
<h3>Introduction</h3> Based on our previous data that the heparan sulfate proteoglycan syndecan-4 (Sdc4) is involved in cartilage breakdown during osteoarthritis, authors analysed distribution and functional role of endochondral ossification. The investigated this process limb development mouse embryogenesis studied its contribution fracture healing adult bones, a some respect recapitulates sequence biological events ossification skeletal development. <h3>Methods</h3> promoter activity...
<h3>Introduction</h3> Micro RNAs (miRNAs) play a crucial role in the regulation of bone metabolism. MiR-146a, an important anti-inflammatory miRNA, was found to negatively impact osteogenesis and regeneration <i>in vitro</i>, by controlling differentiation mesenchymal stem cells. But date miR-146a remodelling, its influence on stability development osteoporosis is not known. <h3>Objectives</h3> The objective this project analysis <h3>Methods</h3> Systemic bone, tibiae femur, wt deficient...
<h3>Career situation of first and presenting author</h3> Post-doctoral fellow. <h3>Introduction</h3> Micro RNAs (miRNAs) play a crucial role in the regulation bone metabolism. MiR-146a, an important anti-inflammatory miRNA, was found to negatively impact osteogenesis regeneration <i>in vitro</i>, by controlling differentiation mesenchymal stem cells. But date miR-146a remodelling, its influence on stability development osteoporosis is not known. <h3>Objectives</h3> Our aim analyse function...
Abstract Bone loss is one of the consequences aging, leading to diseases such as osteoporosis and increased susceptibility fragility fractures therefore considerable morbidity mortality in humans. Here we identify microRNA 146a an essential epigenetic switch controlling bone with age. Mice deficient miR-146a show regular development their skeleton. However, while WT mice start lose age, animals continue accrue throughout life span. Increased mass due generation activity osteoblasts a result...