A5026582019- Osteoarthritis Treatment and Mechanisms
- Rheumatoid Arthritis Research and Therapies
- Cell Adhesion Molecules Research
- Bone Metabolism and Diseases
- Protease and Inhibitor Mechanisms
- Inflammatory mediators and NSAID effects
- Proteoglycans and glycosaminoglycans research
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Immunology Research
- Bone health and treatments
- Peptidase Inhibition and Analysis
- Systemic Lupus Erythematosus Research
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Immune Response and Inflammation
- HER2/EGFR in Cancer Research
- S100 Proteins and Annexins
- NF-κB Signaling Pathways
- Chemokine receptors and signaling
- Connective tissue disorders research
- Virus-based gene therapy research
- Musculoskeletal synovial abnormalities and treatments
- Signaling Pathways in Disease
- Wnt/β-catenin signaling in development and cancer
University Hospital Münster
2016-2025
University of Münster
2014-2023
Institute for Musculoskeletal Health
2018
Universitätsmedizin Greifswald
2014
Universität Hamburg
2014
University Medical Center Hamburg-Eppendorf
2014
Heinrich Heine University Düsseldorf
2014
Düsseldorf University Hospital
2014
Klinik für Schlafmedizin
2007-2012
Medizinische Hochschule Hannover
2009-2010
Abstract Objective Hypertrophic chondrocyte differentiation is a key step in endochondral ossification that produces basic calcium phosphates (BCPs). Although hypertrophy has been associated with osteoarthritis (OA), chondrocalcinosis considered an irregular event and linked mainly to pyrophosphate dihydrate (CPPD) deposition. The aim of this study was determine the prevalence composition crystals human OA analyze their relationship disease severity markers hypertrophy. Methods One hundred...
<h3>Objectives</h3> To compare the efficacy and safety of chondroitin sulfate plus glucosamine hydrochloride (CS+GH) versus celecoxib in patients with knee osteoarthritis severe pain. <h3>Methods</h3> Double-blind Multicentre Osteoarthritis interVEntion trial SYSADOA (MOVES) conducted France, Germany, Poland Spain evaluating treatment CS+GH 606 Kellgren Lawrence grades 2–3 moderate-to-severe pain (Western Ontario McMaster index (WOMAC) score ≥301; 0–500 scale). Patients were randomised to...
Autoimmune diseases, such as psoriasis and arthritis, show a patchy distribution of inflammation despite systemic dysregulation adaptive immunity. Thus, additional tissue-derived signals, danger-associated molecular patterns (DAMPs), are indispensable for manifestation local inflammation. S100A8/S100A9 complexes the most abundant DAMPs in many autoimmune diseases. However, regulatory mechanisms locally restricting DAMP activities barely understood. We now unravel first time, to our...
Blocking TNF effectively inhibits inflammation and structural damage in human rheumatoid arthritis (RA). However, so far it is unclear whether the effect of a direct one or indirect on up-regulation other mediators. IL-1 may be these candidates because has central role animal models arthritis, inhibition used as therapy RA. We removed effects from TNF-mediated inflammatory joint disease by crossing IL-1alpha beta-deficient mice (IL-1-/-) with arthritic TNF-transgenic (hTNFtg) mice....
Objective To analyze the expression pattern of osteoclast differentiation factor (ODF) and its contribution to osteoclastogenesis in rheumatoid arthritis (RA). Methods The ODF was analyzed by reverse transcriptase–polymerase chain reaction (RT-PCR) RA synovial fibroblasts (RASF) isolated from 7 patients normal skin fibroblasts. Using RNA probes specific for ODF, situ hybridization performed. Immunohistochemical double labeling CD68 applied characterize ODF-expressing cells. protein messenger...
For some time synovial fibroblasts have been regarded simply as innocent cells, mainly responsible for homeostasis. During the past decade, however, a body of evidence has accumulated illustrating that rheumatoid arthritis (RASFs) are active drivers joint destruction in arthritis. Details regarding intracellular signalling cascades result long-term activation and synthesis proinflammatory molecules matrix-degrading enzymes by RASFs analyzed. Molecular, cellular animal studies identified...
We reported recently that albumin is a suitable drug carrier for targeted delivery of methotrexate (MTX) to tumors. Due pathophysiological conditions in neoplastic tissue, high amounts accumulate tumors and are metabolized by malignant cells. MTX, covalently coupled human serum (MTX-HSA) cancer treatment, currently being evaluated phase II clinical trials. Because synovium patients with rheumatoid arthritis (RA) shares various features observed also tumors, albumin-based targeting inflamed...
Activation and disruption of Wnt/β-catenin signaling both result in cartilage breakdown via unknown mechanisms. Here we show that WNT-3A the Wnt inhibitor DKK1 induced de-differentiation human articular chondrocytes through simultaneous activation β-catenin-dependent independent responses. activates canonical pathway Ca(2+)/CaMKII noncanonical pathways, with distinct transcriptional targets. promotes cell proliferation loss expression chondrocyte markers COL2A1, Aggrecan, SOX9; however,...
Blocking the Wnt inhibitor sclerostin leads to inflammatory joint destruction through enhanced activation of TNF receptor–mediated signaling, implicating as protective in TNFα-dependent chronic inflammation.
Aberrant immune responses represent the underlying cause of central nervous system (CNS) autoimmunity, including multiple sclerosis (MS). Recent evidence implicated crosstalk between coagulation and immunity in CNS autoimmunity. Here we identify factor XII (FXII), initiator intrinsic cascade kallikrein-kinin system, as a specific cell modulator. High levels FXII activity are present plasma MS patients during relapse. Deficiency or pharmacologic blockade renders mice less susceptible to...
Knock-in genome targeting risks: Comprehensive locus analysis is essential for precision chromosome-editing identification.
In the present study, we searched for mutant PTEN transcripts in aggressive rheumatoid arthritis synovial fibroblasts (RA-SF) and studied expression of RA. By automated sequencing, no evidence presence was found. However, situ hybridization on RA synovium revealed a distinct pattern PTEN, with negligible staining lining layer but abundant sublining. Normal tissue exhibited homogeneous PTEN. cultured RA-SF, only 40% expressed Co-implantation RA-SF normal human cartilage into severe combined...
Sentrin, a novel antiapoptotic molecule, has been shown to interact with the signal-competent form of Fas/APO-1 and tumor necrosis factor receptor I (TNFRI), thereby, protect cells against anti-Fas/APO-1- TNF-induced cell death. Since reduced apoptosis in synovial lining is supposed contribute hyperplasia rheumatoid arthritis (RA), we searched for expression sentrin-1 messenger RNA (mRNA) synovium from patients RA.The mRNA was examined by situ hybridization on snap-frozen sections normal RA...
Objective To study the expression of messenger RNA (mRNA) for different membrane-type matrix metalloproteinases (MT-MMPs) and compare their pattern in rheumatoid arthritis (RA) normal synovium. Methods Polymerase chain reaction (PCR) with specific primers was performed to analyze presence MT1-, MT2-, MT3-, MT4-MMP synovial tissue fibroblasts from 10 patients RA 4 subjects without arthritis. In addition, situ hybridization digoxigenin-labeled probes used investigate MT-MMPs synovium these...