A5086602394- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Rheumatoid Arthritis Research and Therapies
- Peptidase Inhibition and Analysis
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Inflammasome and immune disorders
- Immunodeficiency and Autoimmune Disorders
- Galectins and Cancer Biology
- Vasculitis and related conditions
- Cytokine Signaling Pathways and Interactions
- Signaling Pathways in Disease
- Protease and Inhibitor Mechanisms
- interferon and immune responses
- Chronic Lymphocytic Leukemia Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Hematological disorders and diagnostics
- NF-κB Signaling Pathways
- Inflammatory Myopathies and Dermatomyositis
- Diabetes and associated disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Renal Diseases and Glomerulopathies
- Orthopaedic implants and arthroplasty
University Hospital of Bern
2014-2020
University of Bern
2016-2020
SIB Swiss Institute of Bioinformatics
2018
Medical University of Vienna
2018
National Institutes of Health
2007-2014
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2007-2013
University Hospital of Zurich
1999-2007
University of Zurich
2001-2004
World Health Organization
2003
During T cell-B cell collaboration, plasma (PC) differentiation and Ig production are known to require cell-derived soluble factors. However, the exact nature of cytokines produced by activated cells that costimulate PC is not clear. Previously, we reported costimulation purified human B with IL-21 anti-CD40 resulted in efficient differentiation. In this study, addressed whether de novo was involved direct cell-induced activation, proliferation, We found peripheral blood CD4(+) expressed...
Abstract Objective To characterize the expression pattern and role of galectin 3 binding protein (G3BP) in rheumatoid arthritis (RA), comparison with 1, to explore whether soluble G3BP, investigated serum, synovial fluid, or cell culture supernatant, are associated disease. Methods Synovial tissues from patients RA osteoarthritis (OA), as well healthy controls, were analyzed for galectins 1 G3BP by situ hybridization immunohistochemistry. Levels serum fluid OA supernatants, determined...
Rheumatoid arthritis (RA) is characterized by a progressive destruction of joints invasive synovial fibroblasts (SF). We searched for retroviral sequences in RA fluid pellets, identified sequence similar to that open reading frame 2 (ORF2)/L1 retrotransposable elements, explored the expression L1 tissues and cultured SF, investigated link genomic DNA hypomethylation influence functional on gene expression.RA pellets were screened reverse transcriptase-polymerase chain reaction (RT-PCR) using...
Nuclear factor-κB (NF-κB) controls genes involved in normal lymphocyte functions, but constitutive NF-κB activation is often associated with B cell malignancy. Using high-throughput whole transcriptome sequencing, we investigated a unique family hereditary polyclonal lymphocytosis. We found novel germline heterozygous missense mutation (E127G) affected patients the gene encoding CARD11, scaffolding protein required for antigen receptor (AgR)–induced both and T lymphocytes. subsequently...
Abstract Both constitutive Ig secretion by long-lived plasma cells (PC) and the recurrent differentiation of memory (mem) B into PC contribute to maintenance serologic mem. However, relative contribution each is unknown. In this study, we describe a novel population human postswitched mem that rapidly differentiate thus These IgG+ reside in region spleen analogous murine marginal zone have not previously been examined. are highly responsive IL-21 context CD40 stimulation. Uniquely, analog...
We have identified a distinct pre-naive B cell population circulating in human peripheral blood that exhibits an intermediate phenotype between transitional and naive cells. Like cells, these cells express CD5 but densities of CD38, CD10, CD9, the ABCB1 transporter compared with These account for majority CD5(+) Importantly, could be induced to differentiate into vitro. show only partial responses BCR stimulation CD40 ligation undergo more spontaneous apoptosis death than do whereas...
To analyse magnetic resonance angiographic (MRA) vessel wall signals from a randomized controlled trial of tocilizumab (TCZ) to treat GCA.Participants were assigned in 2:1 ratio receive either TCZ + glucocorticoids (GCs) or placebo GC infusions at 4-week intervals for 52 weeks. GCs started 1 mg/kg/day, then tapered 0.1 mg/kg/day week 12 and thereafter down zero. Patients with initial positive MRA findings underwent control weeks 52. Vessel scored 0 (normal) 3 (intense late enhancement)....
The transcription factor PPAR-γ plays a vital role in the generation of IL-9–producing T H cells.
Objective To study the expression of messenger RNA (mRNA) for different membrane-type matrix metalloproteinases (MT-MMPs) and compare their pattern in rheumatoid arthritis (RA) normal synovium. Methods Polymerase chain reaction (PCR) with specific primers was performed to analyze presence MT1-, MT2-, MT3-, MT4-MMP synovial tissue fibroblasts from 10 patients RA 4 subjects without arthritis. In addition, situ hybridization digoxigenin-labeled probes used investigate MT-MMPs synovium these...
Abstract Objective To study the specific contribution of MAP kinase activator c‐Raf‐1 and one its downstream transcription factors, c‐Myc, to growth invasive behavior rheumatoid arthritis synovial fibroblasts (RASFs). Methods RASFs were transduced with retroviral constructs expressing dominant‐negative mutants or c‐Myc (DN DN respectively) mock vector. The expression wild‐type mutant proteins was confirmed by Western blotting. Growth curves recorded, apoptosis measured flow cytometry....
Tocilizumab is effective in inducing and maintaining remission of GCA. Despite clinical serological control disease, magnetic resonance angiography may show persistence inflammatory signals unknown significance arterial walls. Thus, there an unmet need for tools to detect subclinical disease activity.Immune-inflammatory markers were measured prospectively collected sera the first randomized, double-blind, placebo-controlled trial investigating use tocilizumab As a comparison,...
We characterized a full length L1 mRNA in rheumatoid arthritis (RA) synovial tissue and determined the degree of methylation its 5′-UTR. asked whether not only intact but also altered L1s can exert biological activities by transfecting RA fibroblasts (SF) with either retrotransposition-competent or incompetent examined their capacity to induce p38δ. Total RNA was isolated from 35-year-old woman highly destructive RA. A complete sequence obtained 3′/5′-RACE. Methylation genomic 5′-UTR...