A5047199948- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Chemokine receptors and signaling
- Hematopoietic Stem Cell Transplantation
- Cutaneous Melanoma Detection and Management
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Melanoma and MAPK Pathways
- Lymphatic System and Diseases
- Congenital heart defects research
- Acute Myeloid Leukemia Research
- Bone and Joint Diseases
- Tissue Engineering and Regenerative Medicine
- Apelin-related biomedical research
- MicroRNA in disease regulation
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Neonatal Respiratory Health Research
- Digital Holography and Microscopy
- Zebrafish Biomedical Research Applications
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Bone and Dental Protein Studies
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Nuclear Receptors and Signaling
Max Planck Institute for Molecular Biomedicine
2022-2024
University of Münster
2022-2024
Korea Advanced Institute of Science and Technology
2010-2022
Princeton University
2012-2017
Institute for Basic Science
2016
Chonnam National University Hospital
2016
Keio University
2016
Massachusetts General Hospital
2012
Harvard Stem Cell Institute
2012
Inha University
2011
Abstract Bone regeneration requires a well-orchestrated cellular and molecular response including robust vascularization recruitment of mesenchymal osteogenic cells. In femoral fractures, angiogenesis osteogenesis are closely coupled during the complex healing process. Here, we show with advanced longitudinal intravital multiphoton microscopy that early vascular sprouting is not directly to osteoprogenitor invasion calvarial bone regeneration. Early osteoprogenitors emerging from periosteum...
Tremendous efforts have been made to establish effective therapeutic neovascularization using adipose tissue-derived stromal vascular fraction (SVF), but the efficiency is low, and underlying mechanisms their interaction with host in a new microenvironment are poorly understood.Here we demonstrate that direct implantation of SVF derived from donor tissue can create profound network through disassembly reassembly blood endothelial cells at site implantation. This neovasculature successfully...
Highly metastatic and chemotherapy-resistant properties of malignant melanomas stand as challenging barriers to successful treatment; yet, the mechanisms responsible for their aggressive characteristics are not fully defined. We show that a distinct population expressing CD133 (Prominin-1), which is highly enriched after administration chemotherapeutic drug, dacarbazine, has enhanced potential in vivo. CD133(+) tumor cells located close tumor-associated lymphatic vessels organs such regional...
Vascular endothelial growth factor (VEGF) signaling is a key pathway for angiogenesis and requires highly coordinated regulation. Although the Notch pathway-mediated suppression of excessive VEGF activity via negative feedback well known, positive control augmenting remains poorly understood. Transcription Sox17 indispensable angiogenesis, but its association with largely unknown. The contribution other Sox members to also be determined.To reveal genetic interaction Sox7, another member, in...
Abstract In adult mammalian bone marrow (BM), vascular endothelial cells and perivascular reticular control the function of haematopoietic stem progenitor (HSPCs). During fetal development, mechanisms regulating de novo cell colonization BM remain largely unknown. Here, we show that exhibit fundamental differences in cellular composition molecular interactions by single RNA sequencing. While femur is devoid leptin receptor-expressing cells, arterial (AECs) provide Wnt ligand to initial HSPC...
Abstract The bone marrow microenvironment is a critical regulator of haematopoietic stem cell self-renewal and fate 1 . Although it appreciated that ageing, chronic inflammation other insults compromise function thereby negatively affect haematopoiesis 2 , not known whether different compartments exhibit distinct microenvironmental properties functional resilience. Here we use imaging, pharmacological approaches mouse genetics to uncover specialized in adult ageing skull. Specifically, show...
Abstract Demyelination due to autoreactive T cells and inflammation in the central nervous system are principal features of multiple sclerosis (MS), a chronic highly disabling human disease affecting brain spinal cord. Here, we show that treatment with apelin, secreted peptide ligand for G protein-coupled receptor APJ/Aplnr, is protective experimental autoimmune encephalomyelitis (EAE), an animal model MS. Apelin reduces immune cell entry into brain, delays onset severity EAE. affects...
Abstract Upon severe head injury (HI), blood vessels of the meninges and brain parenchyma are inevitably damaged. While limited vascular regeneration injured has been studied extensively, our understanding meningeal following is quite limited. Here, we identify key pathways governing HI. Rapid complete in predominantly driven by VEGFR2 signaling. Substantial increase observed both human patients mouse models HI, endothelial cell-specific deletion Vegfr2 latter inhibits regeneration. We...
Abstract Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, origin and identity of putative are still unclear. Here, we uncover a novel class lineage cells, PDGFRα + Flk1 − (PCBs), from mouse human pluripotent stem cells induced using CsAYTE, combination small molecules Cyclosporin A, rho-associated coiled-coil kinase inhibitor Y27632, antioxidant Trolox, ALK5 EW7197. This population lineage–committed but in...
Hematopoietic stem cell transplantation (HSCT) is commonly used to treat patients with various blood disorders, genetic and immunological diseases, solid tumors. Several systemic complications following HSCT are critical limiting factors for achieving a successful outcome. These mainly due the lack of initial engraftment after transplantation. However, detailed underlying cellular dynamics early have not been fully characterized yet. We performed in vivo longitudinal visualization...
<title>Abstract</title> The bone marrow microenvironment is a critical regulator of hematopoietic stem cell self-renewal and fate. While it appreciated that aging, chronic inflammation other insults compromise function thereby negatively affect hematopoiesis, not known whether different compartments exhibit distinct microenvironmental properties functional resilience. Here, we have employed imaging, pharmacological approaches, mouse genetics to uncover specialized highly surprising in adult...
<div>Abstract<p>Highly metastatic and chemotherapy-resistant properties of malignant melanomas stand as challenging barriers to successful treatment; yet, the mechanisms responsible for their aggressive characteristics are not fully defined. We show that a distinct population expressing CD133 (Prominin-1), which is highly enriched after administration chemotherapeutic drug, dacarbazine, has enhanced potential <i>in vivo</i>. CD133<sup>+</sup> tumor cells...
<div>Abstract<p>Highly metastatic and chemotherapy-resistant properties of malignant melanomas stand as challenging barriers to successful treatment; yet, the mechanisms responsible for their aggressive characteristics are not fully defined. We show that a distinct population expressing CD133 (Prominin-1), which is highly enriched after administration chemotherapeutic drug, dacarbazine, has enhanced potential <i>in vivo</i>. CD133<sup>+</sup> tumor cells...