A5082164702- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Bioinformatics and Genomic Networks
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Extracellular vesicles in disease
- COVID-19 Clinical Research Studies
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Gene expression and cancer classification
- Virus-based gene therapy research
- Wnt/β-catenin signaling in development and cancer
- SARS-CoV-2 and COVID-19 Research
- Circular RNAs in diseases
- Developmental Biology and Gene Regulation
- Tracheal and airway disorders
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Neurogenesis and neuroplasticity mechanisms
- Tumors and Oncological Cases
- Angiogenesis and VEGF in Cancer
- Head and Neck Cancer Studies
- Climate Change and Health Impacts
- Nerve injury and regeneration
University of Göttingen
2017-2025
Universitätsmedizin Göttingen
2018-2024
Charité - Universitätsmedizin Berlin
2024
University Medical Center
2024
University Hospital Cologne
2024
University of Helsinki
2024
University Hospital Leipzig
2024
Abstract Aims Myocardial fibrosis (MF) might represent a key player in pathophysiology of heart failure aortic stenosis (AS). We aimed to assess its impact on left ventricular (LV) remodelling, recovery, and mortality after transcatheter valve implantation (TAVI) different AS subtypes. Methods results One hundred patients with severe were prospectively characterized clinically echocardiographically at baseline (BL), 6 months, 1 year, 2 years following TAVI. Left biopsies harvested...
The peripheral nervous system harbors a remarkable potential to regenerate after acute nerve trauma. Full functional recovery, however, is rare and critically depends on Schwann cells that orchestrate breakdown resynthesis of myelin and, at the same time, support axonal regrowth. How meet high metabolic demand required for repair remains poorly understood. We here report injury induces adipocyte glial signaling identify adipokine leptin as an upstream regulator adaptation in regeneration....
Research Article10 August 2018Open Access Transparent process Comparative proteomics reveals a diagnostic signature for pulmonary head-and-neck cancer metastasis Hanibal Bohnenberger Institute of Pathology, University Medical Center, Göttingen, Germany Search more papers by this author Lars Kaderali Bioinformatics, Medicine Greifswald, Philipp Ströbel Diego Yepes Department II, Hematology/Oncology, Goethe University, Frankfurt, German Cancer Consortium, Heidelberg, Uwe Plessmann...
Mast cells (MCs) play a crucial role in the tumor microenvironment (TME) of oral squamous cell carcinoma (OSCC), significantly impacting patient prognosis. This study aimed to investigate gene and microRNA (miRNA) expression profiles MCs OSCC following co-culture, providing valuable insights into molecular background their functional interactions. The human line PCI-13 MC LUVA were initially cultured separately under identical experimental conditions subsequently co-cultured for 48-72h....
Breast cancer has been associated with activation of the WNT signaling pathway, although no driver mutations in genes have found yet. Instead, a high expression alternative receptor ROR2 was observed, particular breast brain metastases. However, its respective ligand and downstream this context remained unknown. We modulated human cells characterized their gene protein by RNA-Seq, qRT-PCR, immunoblots reverse phase array (RPPA) combined network analyses to understand molecular basis cancer....
Abstract Single cell multi-omics analysis has the potential to yield a comprehensive understanding of cellular events that underlie basis human diseases. The cardinal feature access this information is technology used for single-cell isolation, barcoding, and sequencing. Most currently RNA-sequencing platforms have limitations in several areas including selection, documentation library chemistry. In study, we describe novel high-throughput, full-length, approach combines CellenONE isolation...
Lymphedema (LE) affects millions of people worldwide. It is a chronic progressive disease with massive development fibrosclerosis when untreated. There no pharmacological treatment lymphedema. The associated swelling the interstitium affected organ, mostly arm or leg, impressive adipose tissue, fibrosis and sclerosis accumulation huge amounts collagen, Papillomatosis cutis. Malnutrition reduced oxygenation tissues hallmark Here, we investigated if hypoxia lymphatic endothelial cells (LECs)...
Abstract Aberrant Wnt activation has been reported in failing cardiomyocytes. Here we present single cell transcriptome profiling of hearts with inducible cardiomyocyte-specific (β-cat Δex3 ) as well compensatory and hypertrophic remodeling. We show that functional enrichment analysis points to an involvement extracellular vesicles (EVs) related processes β-cat mice. A proteomic vivo cardiac derived EVs from identified differentially enriched proteins involving 20 S proteasome constitutes,...
Most clinical diagnostic and genomic research setups focus almost exclusively on coding regions essential splice sites, thereby overlooking other non-coding variants. As a result, intronic variants that can promote mis-splicing events across range of diseases, including cancer, are yet to be systematically investigated. Such investigations would require both transcriptomic data, but there currently exist very few datasets satisfy these requirements. We address this by developing...
ABSTRACT The search for successful therapies of infections with the coronavirus SARS-CoV-2 is ongoing. We tested inhibition host cell nucleotide synthesis as a promising strategy to decrease replication SARS-CoV-2-RNA, thus diminishing formation virus progeny. Methotrexate (MTX) an established drug cancer therapy and induce immunosuppression. inhibits dihydrofolate reductase other enzymes required nucleotides. Strikingly, was inhibited by MTX in therapeutic concentrations around 1 μM,...
The highly conserved YrdC domain-containing protein (YRDC) interacts with the well-described KEOPS complex, regulating specific tRNA modifications to ensure accurate synthesis. Previous studies have linked complex a role in promoting telomere maintenance and controlling genome integrity. Here, we report on newborn severe neonatal progeroid phenotype including generalized loss of subcutaneous fat, microcephaly, growth retardation, wrinkled skin, renal failure, premature death at age 12 days....
ABSTRACT Breast cancer has been associated with activation of the WNT signaling pathway, although underlying molecular mechanisms are still unclear. Here, we found receptor ROR2 to be highly expressed in aggressive breast tumors and worse metastasis-free survival. In order understand basis these observations, overexpressed human cell lines, inducing a BRCAness-like phenotype rendering them resistant PARP inhibition. High levels were defects morphology cell-cell-contacts leading increased...
Abstract Most clinical diagnostic and genomic research setups focus almost exclusively on coding regions essential splice sites, thereby overlooking other non-coding variants. As a result, intronic variants that can promote mis-splicing events across range of diseases, including cancer, are yet to be systematically investigated. Such investigations would require both transcriptomic data, but there currently exist very few datasets satisfy these requirements. We address this by developing...
Pediatric high-grade gliomas (pedHGG) comprise a very poor prognosis. Thus, parents of affected children are increasingly resorting to complementary and alternative medicine (CAM), among those Boswellia extracts. However, nothing is known about the therapeutic effectiveness their active substances, Boswellic acids (BA) in pedHGG. we aimed investigate if three main present plants, alpha-boswellic acid (α-BA), beta-boswellic (β-BA) 3-acetyl-11-keto-beta-boswellic (AKBA) hold any promising...
Highlights•Loss of Prdm12 induces Bax-dependent apoptosis in developing somatosensory ganglia•Prdm12 represses the visceral determinants Phox2a/b ganglia•Repression by is time and context dependent•Visceral fate switch surviving ectopic cell incompleteSummaryPrdm12 a transcriptional regulator essential for emergence somatic nociceptive lineage during sensory neurogenesis. The exact mechanisms which promotes nociceptor development remain, however, poorly understood. Here, we report that...
Prdm12 is a conserved epigenetic transcriptional regulator that displays restricted expression in nociceptors of the developing peripheral nervous system. In mice, required for development entire nociceptive lineage. humans, PRDM12 mutations cause congenital insensitivity to pain, likely because loss nociceptors. maintained mature suggesting yet-to-be explored functional role adults. Using inducible conditional knockout mouse models, we report adult no longer cell survival but continues play...
ABSTRACT Early dorsal telencephalon development is coordinated by an interplay of transcription factors that exhibit a graded expression pattern in neural progenitors. How they function together to orchestrate cortical remains largely unknown. The Emx2 and Dmrta2 genes encode TFs are expressed similar caudomedial high / rostrolateral low gradient the ventricular zone developing with, medial pallium, but not choroid plexus. Their constitutive loss has been shown impart abnormalities, their...
Abstract CHARGE syndrome is an autosomal dominant malformation disorder caused by pathogenic variants in the chromatin remodeler CHD7. Affected are craniofacial structures, cranial nerves and multiple organ systems. Depending on combination of malformations present, its distinction from other congenital disorders can be challenging. To gain a better insight into regulatory disturbances syndrome, we performed RNA-Seq analysis blood samples 19 children with confirmed disease-causing CHD7...
<title>Abstract</title> Mature T-cell lymphomas and leukemias (mTCL) comprise a clinically genetically heterogeneous group of lymphoid malignancies. Most subtypes peripheral leukemic malignancies show an aggressive clinical course poor prognosis. Thus, these diseases urgently require novel therapeutic strategies. Taking advantage recent progress deciphering the genetic basis mTCL, we generated comprehensive database alterations from >1 800 patients with mTCL utilized bioinformatic...
Summary During somatosensory neurogenesis, neurons are born in an unspecialized transcriptional state, with several transcription factors following a broad-to-restricted expression dynamic as development proceeds, supporting neuron subtype identities. The relevance of this temporal refinement remains however unclear, these being selectively involved which they ultimately maintained. Here we found that Dach1 encodes for bona fide factor retained and required tactile neurons. Within developing...
Protein signaling networks are crucial cornerstones in cellular responses. Deregulation causes various diseases, including cancer. One pathway that is frequently deregulated cancer the WNT pathway. It has been shown highly context-dependent and availability of receptors ligands determines downstream signaling. In order to reveal which pathways activated by a specific receptor-ligand combination, we overexpressed non-canonical receptor ROR2 human breast cell line MCF-7 stimulated it with its...
Abstract Histologic classification of pulmonary cancer with squamous cell histology is challenging as reliable immunohistochemical biomarkers are lacking. In particular smokers head and neck can develop both lung metastases primary cancer. However, their differentiation clinically important for therapy risk stratification. Moreover, molecular targeted therapies carcinoma the largely To identify proteomic diagnostic biomarkers, signaling patterns potential novel drug targets we characterized...