A5023815811- Growth Hormone and Insulin-like Growth Factors
- Hypothalamic control of reproductive hormones
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Adrenal Hormones and Disorders
- Biotin and Related Studies
- Neuroendocrine regulation and behavior
- Animal Genetics and Reproduction
- Pituitary Gland Disorders and Treatments
- Biochemical Analysis and Sensing Techniques
- Thyroid Disorders and Treatments
- Effects and risks of endocrine disrupting chemicals
- Reproductive Physiology in Livestock
- Estrogen and related hormone effects
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroscience and Neuropharmacology Research
- Demographic Trends and Gender Preferences
- Birth, Development, and Health
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Medical and Biological Sciences
- Ion channel regulation and function
University of Arkansas for Medical Sciences
2016-2025
University of Alabama at Birmingham
2020-2024
Arkansas Department of Agriculture
2020
Health and Human Development (2HD) Research Network
1983-2019
Center for Translational Molecular Medicine
2019
Northwestern University
1977-2015
University of Nebraska Medical Center
1973-2015
Augusta University
2004
University of Arkansas at Fayetteville
2001-2004
The University of Texas Medical Branch at Galveston
1992-2003
The technique involving use of unlabeled antibody and the peroxidase-antiperoxidase complex was used to identify adrenocorticotropin (ACTH)-secreting cell in anterior pituitary lobe rat localize ACTH it electron microscopically ultrathin sections. is star-shaped, with processes extending around other cells, contains secretory granules a maximal diameter 300 mµ arranged peripherally along plasma membrane. Stain observed on granules, them, Golgi rough endoplasmic reticulum. One day after...
Titration curves were developed with antisera to 17-39 ACTH (adrenocorticotropin) and 1-39 the techniques of radioimmunoassay electron microscopic immunocytochemistry. For latter method, unlabeled antibody peroxidase-antiperoxidase complex immunocytochemical technique was used stain normal rat pituitary intermediate lobes. By standards, antiserum poor quality. Its titration curve exhibited a flat slope it did not bind significant amount labeled antigen beyond 1: 30 dilution. However,...
The storage sites of the pituitary glycoprotein hormones were identified with use electron microscopic immunocytochemical techniques and antisera to beta (beta) chains follicle-stimulating hormone (FSH), luteinizing (LH) thyroid-stimulating (TSH). TSH cells in normal rats is ovoid or angular contains small granules 60-160 nm diameter. In hypertrophied 45 days after thyroidectomy, staining globular patches diffusely distributed expanded profiles dilated rough endoplasmic reticulum....
Abstract To provide a multi-omics resource and investigate transcriptional regulatory mechanisms, we profile the transcriptome, chromatin accessibility, methylation status of over 70,000 single nuclei (sn) from adult mouse pituitaries. Paired snRNAseq snATACseq datasets individual animals highlight continuum between developmental epigenetically-encoded cell types transcriptionally-determined transient states. Co-accessibility analysis-based identification putative Fshb cis-regulatory domain...
The peripheral nervous system harbors a remarkable potential to regenerate after acute nerve trauma. Full functional recovery, however, is rare and critically depends on Schwann cells that orchestrate breakdown resynthesis of myelin and, at the same time, support axonal regrowth. How meet high metabolic demand required for repair remains poorly understood. We here report injury induces adipocyte glial signaling identify adipokine leptin as an upstream regulator adaptation in regeneration....
Follistatin, a glycosylated single chain protein that was originally isolated from ovarian follicular fluid, can specifically inhibit the biosynthesis and secretion of FSH by pituitary. Follistatin has also been bovine pituitary shown to have activin-binding activity. We wished determine whether follistatin gene is expressed in rat and, if so, identify specific cell types. A 337-basepair fragment cDNA amplified polymerase reaction library subcloned into pGEM3. Low levels mRNA poly(A)+RNA...
Separation of gonadotrophs from cell suspensions adult female rat pituitary glands by centrifugal elutriation was applied to the preparation gonadotropin-enriched cells. Trypsin-dispersed cells (200–300 million) were loaded into elutriator chamber while rotor operating at 1960 rpm, and separated collected in fractions increasing flow rate medium through increments 11.8 39.5 ml/min. Loading completed 80 min; recovery 78/95%. Immunocytochemical staining initial suspension (ICS)...
The avidin-biotin-peroxidase complex (ABC) method was applied to semithin (0.5-1 micron) plastic-embedded sections of intact male rat pituitaries with the use techniques previously developed for peroxidase-antiperoxidase (PAP) method. Stains adrenocorticotropin (ACTH), thyroid stimulating hormone (TSH), luteinizing (LH), and follicle (FSH) were cleaner, more reliable, efficient. ABC allowed same high dilutions primary antisera used PAP Incubation time cut a third stain. Furthermore, if...
Anterior pituitary cells were studied with irrimunocytochemical methods 1–6 months after gonadectomy in male and female rats. One month surgery, stained gonadotropes contained small scattered cisternae of rough endoplasmic reticulum, while others showed larger cisternae. The signet ring observed 3 gonadectomy, their numbers increased markedly by 6 months. Counts freshly dispersed that intact rats, the only significant sex differences seen percentages FSH PRL cells. females had higher both...
A new synthetic biotinylated N-terminal analog of CRF was used to study its binding, endocytosis, and route processing. The fully bioactive compared rat(r) in an ACTH bioassay pituitary membrane receptor assay. In 1- 4-day cultured cell monolayers, receptors were labeled for 1-biotinylated rCRF (1-bio-CRF) demonstrated with avidin-fluorescein (Ar-Fl) living cells or avidin-biotin-peroxidase complex (ABC) fixed cells. percentages fluorescein-labeled comparable those stained the ABC technique....
Physiological and morphological changes produced by corticotropin-releasing factor (CRF) stimulation in vivo were studied pituitaries immunocytochemically stained for ACTH. After 48-h ip minipump infusions of 10 or 50 ng/min CRF, serum ACTH levels increased significantly over values control groups that included both intact rats exposed to sham abdominal surgery. Correlative a striking increase corticotrope cell area. This was coupled with an apparent decrease the percentage cells, probably...
Araldite sections of rat pituitary intermediate lobe were used with anti- 17-39 adrenocorticotropin in the unlabeled antibody enzyme method to compare electron microscopic immunocytochemical staining by peroxidase-antiperoxidase complex (PAP) antiserum or purified peroxidase followed (PO). Quantitation normalized optical densities secretory granules offered high significance comparison (P < 0.0001) experimental control values and each other. Use (prepared a new density gradient method)...
Morphological criteria have been used by Kurosumi and Oota to distinguish folliclestimulating hormone (FSH) from luteinizing (LH) cells in the rat pituitary. In this study techniques of ultrastructural immunocytochemistry were determine if these cell types could be distinguished on basis their LH content. female rats diestrus a 1:125,000 dilution anti-bLH-β stained Kurosumi-Oota “LH cells,” “FSH with some morphological characteristics both types. Absorption 10 ng abolished staining all...
The adipokine leptin signals the body's nutritional status to brain and particularly, hypothalamus. However, receptors can be found all throughout body, including pituitary. It is known that permissive for reproduction, mice cannot produce (Lep/Lep) are infertile. Many studies have pinpointed leptin's regulation of reproduction exist at levels hypothalamic-pituitary-gonadal axis. We previously shown deleting signaling portion receptor specifically in gonadotropes impairs fertility female...
Immunocytochemical staining for serotonin, 5-hydroxytryptamine, in frozen sections of the pituitary resulted localization fine, varicose fibers specific regions adenohypophysis. Stained with beaded varicosities were observed entering rostral zone adenohypophysis blood vessels, coursing over surface anterior lobe, and splitting into deeper portions lobe. In both horizontal sagittal sections, extending from periphery gland to penetrate between first 2–3 cell layers. The intermediate lobe was...
The application of centrifugal elutriation to the separation pituitary cells has produced a fraction that is enriched with LH and FSH gonadotropes. In this study, stains were performed on serial sections fractions taken from six experiments 1:10,000–1:30,000 anti-bLHβ or 1:2,000–1:8,000 anti-hFSHβ avidin-biotin-peroxidase complex technique. Over 900 serially sectioned gonadotropes analyzed. initial cell suspensions, 59.6% contained both FSH; 18% LH, 23% FSH. Fewer than 3% ACTH. several...
The neonatal rat exhibits a stress-nonresponsive period during days 4–11 of postnatal development. role and response the anterior lobe corticotrope was studied with immunocytochemical stains for ACTH on fixed embedded pituitaries from male rats 2–21 age. Serially sectioned fields were stained β-chains LH or FSH to test joint storage one both gonadotropins. Cell counts semithin sections used determine frequency corticotropes in developing pituitary. 2-day-old had twice as many (17.6%) adults...
Abstract This study was designed to elucidate hormone storage patterns in gonadotropes with the use of ultrastructural immunocytochemistry on serial ultrathin sections. Sets six sections were stained for beta chains LH, FSH, or C‐Terminal sequence ACTH, and 430 cells cut triple double section collected from a group seven normal adult male rats. Approximately 50‐88% contained both LH most these Type I which are distinguished by their round shape heterogeneous populations secretion granules....
Abstract Anterior pituitary somatotropes respond to metabolic signals from the adipokine leptin optimize functional responses body’s nutritional state via growth hormone (GH) secretion. Molecular targets of in include GH, GH-releasing receptor (GHRHR), and, females, transcription factor POU1F1, all which are dependent on stimulation for expression. To identify trophic mechanisms underlying action upon somatotropes, we analyzed single-cell gene transcriptomes comparing pituitaries a female...
The Musashi family of sequence-specific RNA binding proteins (Musashi1 and Musashi2) serve a critical role in mediating both physiological pathological stem cell function many tissue types by repressing the translation target mRNAs that encode promote cycle inhibition differentiation. In addition to repression mRNAs, we have also identified for activating context-dependent manner. However, molecular mechanisms which controls mRNA translational activation not been fully elucidated. Since...