A5047399103- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
Icahn School of Medicine at Mount Sinai
2023
Princeton University
2021-2023
Abstract To provide a multi-omics resource and investigate transcriptional regulatory mechanisms, we profile the transcriptome, chromatin accessibility, methylation status of over 70,000 single nuclei (sn) from adult mouse pituitaries. Paired snRNAseq snATACseq datasets individual animals highlight continuum between developmental epigenetically-encoded cell types transcriptionally-determined transient states. Co-accessibility analysis-based identification putative Fshb cis-regulatory domain...
Single same cell RNAseq/ATACseq multiome data provide unparalleled potential to develop high resolution maps of the cell-type specific transcriptional regulatory circuitry underlying gene expression. We present CREMA, a framework that recovers full cis-regulatory by modeling expression and chromatin activity in individual cells without peak-calling or type labeling constraints. demonstrate CREMA overcomes limitations existing methods fail identify about half functional elements which are...