Dental implants are one of the most frequently used treatment options for tooth replacement. Approximately 30% patients with dental develop peri-implantitis, which is an oral inflammatory disease that leads to loss supporting tissues, predominately bone. For development future therapeutic strategies, it essential understand molecular pathophysiology human peri-implant infections. Here, we describe gene and protein expression patterns peri-implantitis bone tissue compared healthy tissue....

Degeneration of the knee joint during osteoarthritis often begins with meniscal lesions. Meniscectomy, previously performed extensively after injury, is now obsolete because inevitable that occurs following this procedure. Clinically, meniscus self-renewal well documented as long outer, vascularized ring remains intact. In contrast, regeneration inner, avascular does not occur. Here, we show cartilage tissue harvested from inner human late stages harbors a unique progenitor cell population....

Abstract Changes in the protein expression pattern of osteoblastic lineage cells from alveolar bone (OLAB) during medication‐related osteonecrosis jaw (MRONJ) have rarely been investigated. This lack information is partly because limited availability healthy samples and human cell lines for research. The aim present study was to investigate proteins collagen 1, runt‐related transcription factor 2 (RUNX2), tumor necrosis ligand superfamily member 11 (RANKL). Furthermore, we established a OLAB...

Mast cells (MCs) play a crucial role in the tumor microenvironment (TME) of oral squamous cell carcinoma (OSCC), significantly impacting patient prognosis. This study aimed to investigate gene and microRNA (miRNA) expression profiles MCs OSCC following co-culture, providing valuable insights into molecular background their functional interactions. The human line PCI-13 MC LUVA were initially cultured separately under identical experimental conditions subsequently co-cultured for 48-72h....

Background The COVID-19 pandemic necessitated the widespread use of face masks in medical settings. In treatment patients with head and neck tumors or other malignancies, where non-verbal communication emotional expression are critical, may potentially impair physician-patient relationship. This study aimed to assess impact on perceived empathy quality this patient cohort. Methods A prospective case-control as part postoperative follow-up consultations was conducted at Department Oral...

Abstract Aim The present in vitro study was designed to evaluate the surface characteristics, biocompatibilities and antimicrobial effects of experimental titanium implant surfaces, coated by nanocrystalline silver, copper, bismuth. Biocompatible modifications could result reduced biofilm formation on surfaces therefore less periimplant inflammation. Findings Titanium discs (thickness 1 mm 12 diameter) were pulsed magnetron‐sputtering metals (bismuth, silver). Bismuth coatings revealed...

Mesenchymal stem cells are known to exert immunomodulatory effects in inflammatory diseases. Immuneregulatory lead progressive joint destruction rheumatoid arthritis (RA). Proinflammatory cytokines, such as tumour necrosis factor α (TNF-α) and interleukins (ILs) the main players. Here, we studied progenitor from RA cartilage (RA-CPCs) that positive for IL-17 receptors determinate of inflammation on their chondrogenic potenial. IL-17A/F reduced potential these via upregulation RUNX2 protein...

Summary Osteoarthritis (OA) is the most common chronic joint disease and leads to degradation of extracellular matrix by an imbalance between anabolic catabolic processes. TGF-β3 (transforming growth factor beta-3) epidermal (EGF) influence osteochondrogenic potential chondrocytes. In this study, we compared expression mediators receptors in EGF pathways, as well biglycan (BGN), healthy diseased Furthermore, used chondrogenic progenitor cells (CPCs) for vitro stimulation knockdown...

Changes in the proteome of oral cells during periodontitis have rarely been investigated. This lack information is partially attributed to human cell lines derived from cavity for vitro research. The objective present study was create relevant tissues and compare protein expression cultured alone co-cultivated with periodontitis-associated bacterial strains.We established gingival keratinocytes, osteoblastic lineage alveolar bone, periodontal ligament fibroblasts, cementum cells. Using...

Abstract Objective The aim of the present study was to assess need for secondary palatal corrective surgery in a concept palate repair that uses protocol anterior posterior closure primary palate, hard and soft palate. Methods A data base patients primarily operated between 2001 2021 at Craniofacial Cleft Care Center University Goettingen evaluated. lips had been repaired using Tennison Randall Veau-Cronin procedures conjunction with alveolar cleft repair. CLP accomplished two steps first...

The aim of the present study was to objectively assess degree residual facial asymmetry after primary treatment non-syndromic unilateral cleft lip and palate (UCLP) in children correlate it with subjective ratings appearance.

Oral cancer therapy is associated with a loss in health‑related quality of life (HRQOL) and can also lead to post‑traumatic growth (PTG). The current study analyzed the relationship between HRQOL, PTG influencing clinical factors after treatment. coherent data 15 patients were retrospectively over 1‑year period. HRQOL studied using University Washington Quality Life Version 4 (UW‑QOL v4) Posttraumatic Growth Inventory (PTGI) questionnaires. results revealed that was significantly decreased...

The present study aimed to assess the concordance of preoperative and postoperative hard soft tissues in patients with advanced oral squamous cell carcinoma (OSCC) following virtual surgical planning (VSP) mandibular reconstruction. In study, a cohort 32 OSCC underwent in-house VSP, followed by guided reconstruction utilizing vascularized free tissue grafts sourced from fibula or scapula. A morphometric analysis was conducted comparing three-dimensional models evaluate discrepancies identify...

Localized jawbone invasion is a milestone in the progression of oral squamous cell carcinoma (OSCC). The factors that promote this process are not well understood. Sclerostin known to be involved bone metabolism and there preliminary reports its involvement tumors metastasis. To identify possible sclerostin OSCC, expression was analyzed vitro two different human OSCC tumor lines by quantitative real-time polymerase chain reaction (qRT-PCR), effect recombinant (rh)-sclerostin treatment on...

This study was designed to identify changes in the expression of proteins occurring during progression oral squamous cell carcinoma (OSCC) and validate their impact on patient prognosis.The human OSCC line UPCI-SCC-040 treated vitro with TGF-β1, transcriptome analysis differentially expressed genes (DEGs) revealed putative candidates relative untreated cells. The respective protein levels most important were immunohistochemically validated a tissue microarray (TMA) containing samples from 39...

Objective The aim of this study was to investigate the roles SMURF1 and SMURF2 in progenitor cells from human knee late-stage osteoarthritis (OA). Design We applied immunohistochemistry, immunocytochemistry, RNAi, lentiviral transfection, Western blot analysis. obtained chondrogenic (CPCs) articular cartilage meniscus (MPCs) nonvascularized part meniscus. Results appeared both osteoarthritic tissues. CPCs MPCs exhibited comparable amounts these proteins, which influence balance between RUNX2...

Clefts in newborns are associated with severe morphological and functional impairment. Especially the lip is of importance as if treatment result unsatisfactory, it can lead to psychological changes patient. Different operative procedures have been developed over last decades. The aim presented study was comparison surgical techniques according Millard Pfeifer regarding temporal development postoperative symmetry height mouth width.Digitized photographs patients from department oral...

Periodontitis refers to inflammatory disease of the periodontal structures (the gingiva, dental cementum, ligament (PDL) and alveolar bone) that ultimately leads their destruction. Whereas collagens are well-examined main components periodontium, little is known about other structural proteins make up this tissue. The aim study was identify new extracellular matrix (ECM) components, including fibulins matrilins, in periodontium mice. After sacrificing 14 mice (Sv/129 strain), jaws were...