A5042919854- Cellular transport and secretion
- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Telomeres, Telomerase, and Senescence
- Force Microscopy Techniques and Applications
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- RNA modifications and cancer
- Integrated Circuits and Semiconductor Failure Analysis
- RNA and protein synthesis mechanisms
- Cardiac electrophysiology and arrhythmias
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
Universitätsmedizin Göttingen
2021-2024
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2024
University of Göttingen
2024
We present novel workflows for Q-FISH nanoscopy with the potential prognostic applications and resolving chromatin compaction changes. DNA-fluorescence in situ hybridization (DNA-FISH) is a routine application to visualize telomeres, repetitive terminal DNA sequences, cells tissues. Telomere attrition associated inherited acquired diseases, including cancer cardiomyopathies, frequently analyzed by quantitative (Q)-FISH microscopy. Recently, nanoscopic imaging techniques have resolved...
Abstract The clustering of L-type calcium channels in cardiac myocytes presents an important mechanism for functional regulation signaling. Here we applied targeted super-resolution imaging techniques the study atrial-specific Ca V 1.3 channel clusters human iPSC-derived atrial cardiomyocytes (hiPSC-aCM). We thereby clarified cluster localization, dimensions, architecture, and dynamics, which were largely unexplored previously. Live-cell STimulated Emission Depletion (STED) identified that...
Abstract A key player of excitable cells in the heart and brain is L-type calcium channel Ca V 1.3. In heart, it required for voltage-dependent 2+ -signaling, i.e., controlling modulating atrial cardiomyocyte excitation-contraction coupling. The clustering 1.3 functionally relevant multimers has not been addressed due to a lack stoichiometric labeling combined with high-resolution imaging. Here, we developed HaloTag-labeling strategy visualize quantify clusters using STED nanoscopy address...
The highly conserved YrdC domain-containing protein (YRDC) interacts with the well-described KEOPS complex, regulating specific tRNA modifications to ensure accurate synthesis. Previous studies have linked complex a role in promoting telomere maintenance and controlling genome integrity. Here, we report on newborn severe neonatal progeroid phenotype including generalized loss of subcutaneous fat, microcephaly, growth retardation, wrinkled skin, renal failure, premature death at age 12 days....
Abstract Encoding of several sensory modalities into neural signals is mediated by ribbon synapses. The synaptic tethers vesicles at the presynaptic active zone (AZ) and might act as a super-scaffold organizing AZ topography. Here we employed synthetic biology approach to reconstitute ribbon-type AZs in HEK293 cells for probing their minimal molecular requirements studying Ca 2+ channel clustering. Co-expressing membrane-targeted version AZ-protein Bassoon core protein RIBEYE, observed...
Encoding of several sensory modalities into neural signals is mediated by ribbon synapses. The synaptic tethers vesicles at the presynaptic active zone (AZ) and might act as a super-scaffold organizing AZ topography. Here we employed synthetic biology approach to reconstitute ribbon-type AZs in HEK293 cells for probing their minimal molecular requirements studying Ca 2+ channel clustering. Co-expressing membrane-targeted version AZ-protein Bassoon core protein RIBEYE, observed structures...
Encoding of several sensory modalities into neural signals is mediated by ribbon synapses. The synaptic tethers vesicles at the presynaptic active zone (AZ) and might act as a super-scaffold organizing AZ topography. Here we employed synthetic biology approach to reconstitute ribbon-type AZs in HEK293 cells for probing their minimal molecular requirements studying Ca 2+ channel clustering. Co-expressing membrane-targeted version AZ-protein Bassoon core protein RIBEYE, observed structures...