A5039188051- Extracellular vesicles in disease
- Cardiac electrophysiology and arrhythmias
- MicroRNA in disease regulation
- Mass Spectrometry Techniques and Applications
- Sperm and Testicular Function
- Cardiovascular Effects of Exercise
- RNA Research and Splicing
- Circular RNAs in diseases
- Kruppel-like factors research
- Male Reproductive Health Studies
- Ion channel regulation and function
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Nuclear Receptors and Signaling
- Reproductive Health and Technologies
- Receptor Mechanisms and Signaling
German Centre for Cardiovascular Research
2022-2025
University of Göttingen
2023-2024
Universitätsmedizin Göttingen
2023-2024
Abstract Aberrant Wnt activation has been reported in failing cardiomyocytes. Here we present single cell transcriptome profiling of hearts with inducible cardiomyocyte-specific (β-cat Δex3 ) as well compensatory and hypertrophic remodeling. We show that functional enrichment analysis points to an involvement extracellular vesicles (EVs) related processes β-cat mice. A proteomic vivo cardiac derived EVs from identified differentially enriched proteins involving 20 S proteasome constitutes,...
The identification of novel drug targets is needed to improve the outcomes heart failure (HF). G-protein-coupled receptors (GPCRs) represent largest family for already approved drugs, thus providing an opportunity repurposing. Here, we aimed (i) investigate differential expressions 288 cardiac GPCRs via droplet digital PCR (ddPCR) and bulk RNA sequencing (RNAseq) in a rat model left ventricular pressure-overload; (ii) compare RNAseq findings with those ddPCR; (iii) screen test novel,...
Over the last few years, interest in extracellular vesicles (EVs) function has exponentially grown. However, methods for isolating these small from tissue are still not trivial. Few protocols that allow EV isolation whole samples, including heart, available and they based on organ perfusion unsing Langendorff method. In this work, aiming at analysing vivo biology of EVs, we implemented a simple method to obtain enrichment murine heart tissue. We tested titration Liberase digestion, which was...