SOX9 is a transcription factor of the SRY family that regulates sex determination, cartilage development and numerous other developmental events. In foetal growth plate, Sox9 highly expressed in chondrocytes proliferating prehypertrophic zone but declines abruptly hypertrophic zone, suggesting downregulation might be necessary step to initiate cartilage-bone transition plate. order test this hypothesis, we generated transgenic mice misexpressing under control BAC-Col10a1 promoter. The...

Abstract Sporadic apoptosis of tumour cells is a commonly observed feature colorectal cancer (CRC) and strongly correlates with adverse patient prognosis. The uptake apoptotic cell debris by neutrophils induces non-inflammatory, pro-regenerative, hence potentially pro-tumorigenic phenotype. In this study, we therefore sought to investigate the impact CRC on its consequence other immune microenvironment. Apoptosis induced combined TNFα-treatment UV-C irradiation, as well various...

Abstract Remodelling of the extracellular matrix is accomplished by altering balance between macromolecule production and degradation. However, it not well understood how cells new molecules degradation existing ones during tissue remodelling regeneration. In this study, we used decellularized lung scaffolds repopulated with allogenic fibroblasts cultured stable isotope labelled amino acids to quantify at a proteome-wide scale. Specific temporal dynamics different matrisome proteins were...

The articular cartilage of synovial joints ensures friction-free mobility and attenuates mechanical impact on the joint during movement. These functions are mediated by complex network extracellular molecules characteristic for cartilage. Zonal differences in matrix (ECM) well recognized. However, knowledge about precise molecular composition different zones remains limited. In present study, we investigated distribution ECM along surface-to-bone axis, using quantitative non-targeted as...

Fibroblasts are the most abundant stromal constituents of tumour microenvironment in primary as well metastatic colorectal cancer (CRC). Their supportive effect on cells is established. There growing evidence that fibroblasts also modulate immune tumours. Here, we demonstrate a difference fibroblast-mediated modulation between CRC and peritoneal metastasis. Cancer-associated (CAFs) were isolated from metastases (MAFs) total 17 patients. The ectoenzyme CD38 was consistently expressed surface...

Summary Background Peritoneal mesothelioma (PM) is a rare disease with various histopathological subtypes. For malignant peritoneal and borderline subgroups locoregional therapy cytoreductive surgery (CRS) hyperthermic intraperitoneal chemotherapy (HIPEC) has been implemented. The aim of our study was to retrospectively present the outcome after CRS HIPEC for patients different subtypes mesothelioma. Methods In total 15 received due at tertiary referral hospital between 2013 2022. Surgical...

Cytoreductive surgery (CRS) and heated intraperitoneal chemotherapy (HIPEC) is associated with significant postoperative complications. Early detection of at-risk patients may lead to improved outcomes. The role C-reactive protein (CRP) in predicting complications has only been recently investigated.Postoperative were categorized according Clavien-Dindo classification further divided into minor (Grade <3) major ≥3A). Absolute CRP counts (mg/L) on days (POD) 1-7, proportional change was...

Background A test for diagnosis of active Tuberculosis (TB) from peripheral blood could tremendously improve clinical management patients. Methods Of 178 prospectively enrolled patients with possible TB, 60 were diagnosed pulmonary and 27 extrapulmonary TB. The frequencies Mycobacterium tuberculosis (MTB) specific CD4+ T cells CD8+ producing cytokines assessed using overnight stimulation purified protein derivate (PPD) or early secretory antigenic target (ESAT)-6, respectively. Results Among...

Background Pulmonary hypoplasia, characterized by incomplete alveolar development, remains a major cause of mortality and morbidity in congenital diaphragmatic hernia. Recently demonstrated to differentiate from common bipotent progenitor during the two cell types that line alveoli type 1 2 cells have shown alter their relative ratio hernia lungs. Objective We used nitrofen/bisdiamine mouse model induce accurately assess status epithelial differentiation relation progenitors. Study design...

Purpose Little is known about the perioperative dynamic of albumin and its effect on surgical outcome in Crohn's disease. Thus, we aimed to assess postoperative changes levels their potentially predictive role for complications after laparoscopic intestinal resections. Methods We identified 182 patients who underwent resection symptomatic Crohn´s disease between 2000 2014 this retrospective cohort study. Pre- serum (within 4 days) were recorded retrospectively proportional reduction (delta...

Chronic lung allograft dysfunction in the form of bronchiolitis obliterans syndrome (BOS) is main cause death beyond 1-year post-lung transplantation. The disease-initiating triggers as well molecular changes leading to fibrotic alterations transplanted are largely unknown. aim this study was identify potential early extracellular matrix (ECM) different compartments prior development BOS.Transbronchial biopsies from a cohort 58 transplantation patients at Copenhagen University hospital...

Robotic surgery holds particular promise for complex oncologic colorectal resections, as it can overcome many limitations of the laparoscopic approach. However, similar to situation in surgery, appropriate case selection (simple vs. complex) with respect actual robotic expertise team may be a critical determinant outcome. The present study aimed analyze clinical outcome after over time based on complexity surgical procedure.All resections (n = 85) performed at Department Surgery, Medical...

Brain metastases (BM) are a rare complication in colorectal cancer (CRC) patients and associated with an unfavorable survival prognosis. Primary tumor side (PTS) was shown to act as prognostic predictive biomarker several trials including metastatic CRC (mCRC) patients. Here, we aim investigate whether PTS is also the outcome of BM.Patients treated for BM between 1988 2017 at academic care center were included. Right-sided defined located appendix, cecum ascending colon left-sided descending...

Mycobacterium tuberculosis (MTB)-specific cytokine responses in the peripheral blood and at site of infection may differ significantly within same individual, but under-lying T-cell subset changes are largely unknown. Here, we measured effector memory markers on CD4⁺ T cells (CD45RO, cysteine chemokine receptor (CCR)7, CD27) active (TB). Additionally, were stimulated overnight with purified protein derivative (PPD) early secretory antigenic target (ESAT)-6 to determine which produces...

The risk factors that predict surgical recurrence in Crohn's disease (CD) remain controversial. Postoperative anti-tumour necrosis factor (anti-TNF) therapy might lower rates whilst the presence of mesenteric granulomas has been postulated to increase risk. We hypothesized indicate severity and recurrence, irrespective immunosuppressive therapy.We performed a retrospective review all consecutive patients undergoing operations for CD between January 2000 December 2014 at single tertiary...

Bronchiolitis obliterans syndrome, a common form of chronic lung allograft dysfunction, is the major limitation to long-term survival after transplantation. The histologic correlate progressive, fibrotic occlusion small airways, obliterative bronchiolitis lesions, which ultimately lead organ failure. molecular composition these lesions unknown. In this sutdy, protein in explanted lungs from four end-stage syndrome patients was analyzed using laser-capture microdissection and optimized sample...

We report a case of septic shock syndrome caused by Streptococcus pneumoniae in patient who had undergone splenectomy due to an autoimmune lymphoproliferative (ALPS), which is characterized as dysfunction immunoregulation. Although the was vaccinated with conjugated polysaccharide vaccine after splenectomy, he still susceptible S. infection, because isolated serovar (24F), long thought be apathogenic, not covered any currently approved, neither nor unconjugated one. This demonstrates that,...

Abstract Background Sacral neuromodulation (SNM) is an established treatment option for patients with faecal incontinence. The location of the stimulating electrode considered to be essential success. purpose this study was evaluate position SNM electrodes after using a standardized fluoroscopy-guided implantation technique. Methods For cadaver study, were implanted bilaterally in 5 lower body specimens. edge sacroiliac joint and medial sacral foramina marked fluoroscopy draw ‘H’ crossing...

Summary Background The rate of restoration intestinal continuity after colonic resection and stoma creation in patients with Crohn’s disease has not been well-documented the era biologics. Thus, incidence since introduction biological drugs was assessed. Methods Consecutive ( n = 43) who underwent ileostomy or colostomy formation for at a single tertiary referral center between 2002 2014 were identified. Data from individual chart review analyzed retrospectively. Patients personally...

Abstract Aim Ventral rectopexy ( VR ) has gained popularity in the management of obstructive defaecation syndrome ODS due to a symptomatic rectocele ± intussusception. Data on efficacy and safety are variable there few predictors successful outcome. This study aimed examine whether or not an adverse obstetric history influenced functional outcome following for . Method was retrospective cohort 76 consecutive patients who had undergone at tertiary referral centre between 2012 2015. Patients...