A5028407671- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Medical Imaging and Pathology Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Mast cells and histamine
- Inhalation and Respiratory Drug Delivery
- Pulmonary Hypertension Research and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Allergic Rhinitis and Sensitization
- 3D Printing in Biomedical Research
- Liver physiology and pathology
- Lipid metabolism and biosynthesis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Lipoproteins and Cardiovascular Health
- Metabolomics and Mass Spectrometry Studies
- Transplantation: Methods and Outcomes
- Occupational exposure and asthma
- Biodiesel Production and Applications
- Peptidase Inhibition and Analysis
- Molecular Junctions and Nanostructures
- Diamond and Carbon-based Materials Research
Lund University
2018-2024
Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair of damaged bile ducts. However, typically cultured in mouse tumor-derived basement membrane extracts (BME), which is poorly defined, highly variable and limits the direct clinical applications patients. Extracellular matrix (ECM)-derived hydrogels prepared from decellularized human or porcine livers attractive alternative culture substrates. Here, expansion liver ECM(LECM)-derived described....
Mast cells may activate fibroblasts and contribute to remodeling processes in the lung. However, mechanism behind these actions needs be further investigated. Fibroblasts are major regulators of on-going processes. Protease activated receptor 2 (PAR2) expressed by serine proteases, such as mast cell mediator tryptase. The objective this study was investigate effects specifically tryptase on fibroblast migration role PAR2 activation.Human lung (HFL-1) were cultured together with human...
Abstract Remodelling of the extracellular matrix is accomplished by altering balance between macromolecule production and degradation. However, it not well understood how cells new molecules degradation existing ones during tissue remodelling regeneration. In this study, we used decellularized lung scaffolds repopulated with allogenic fibroblasts cultured stable isotope labelled amino acids to quantify at a proteome-wide scale. Specific temporal dynamics different matrisome proteins were...
In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions. Scaffolds (decellularized tissue) derived from subpleural healthy IPF lungs were examined regarding biomechanical ECM composition proteins (the matrisome). repopulated with fibroblasts cultured under static stretch heavy isotope amino acids (SILAC), to examine newly synthesized over time. scaffolds characterized by...
Cholangiocarcinoma (CCA) is a type of liver cancer with an aggressive phenotype and dismal outcome in patients. The metastasis CCA cells to distant organs, commonly lung lymph nodes, drastically reduces overall survival. However, mechanistic insight how invades these metastatic sites still lacking. This partly because currently available models fail mimic the complexity tissue-specific environments for CCA. To create vitro model which interactions between epithelial tumor their surrounding...
Abstract Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types cardiovascular diseases. These materials have the potential to maintain functional properties extracellular matrix (ECM), and allow growth remodeling in vivo . The most commonly used methods decellularization are based on chemicals enzymes combinations, which often damage ECM cause cytotoxic effects Mild involving pressurized CO 2 -ethanol (EtOH)-based fluids,...
Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate effect of on fibroblast activity migration cells. Lung from IPF patients healthy individuals co-cultured with LAD2 or stimulated proteases tryptase chymase. Human lung cultured cell culture plastic plates decellularized human tissue (scaffolds) create a more physiological milieu by...
Abstract Alveolar epithelial cells (AEC) have been implicated in pathological remodelling. We examined the capacity of AEC to produce extracellular matrix (ECM) and thereby directly contribute towards remodelling chronic lung diseases. Cryopreserved type 2 (AEC2) from healthy lungs obstructive pulmonary disease (COPD) afflicted were cultured decellularized human slices for 13 days. Healthy-derived AEC2 treated with transforming growth factor ß1 (TGF-β1) evaluate plasticity their ECM...
Breathing exposes lung cells to continual mechanical stimuli, which is part of the microenvironmental signals directing cellular functions together with extracellular matrix (ECM). Therefore, developing systems that incorporate both stimuli urgent fully understand cell behavior. This study aims introduce a novel in vitro culture methodology combining cyclic stretch simulates vivo breathing 3D platforms form decellularized slices (DLS) and precision cut (PCLS). To this end, we have...
Bronchiolitis obliterans syndrome, a common form of chronic lung allograft dysfunction, is the major limitation to long-term survival after transplantation. The histologic correlate progressive, fibrotic occlusion small airways, obliterative bronchiolitis lesions, which ultimately lead organ failure. molecular composition these lesions unknown. In this sutdy, protein in explanted lungs from four end-stage syndrome patients was analyzed using laser-capture microdissection and optimized sample...
A subset of individuals with allergic asthma develops a late phase response (LPR) to inhaled allergens, which is characterized by prolonged airway obstruction, inflammation and hyperresponsiveness. The aim this study was identify changes in the plasma proteome circulating hematopoietic progenitor cells associated LPR following allergen challenge. Serial samples from asthmatics undergoing challenge were analyzed mass spectrometry immunosorbent assays. Peripheral blood mononuclear flow...
Background. A subset of individuals with allergic asthma develops a late phase response (LPR) to inhaled allergens, which is characterized by prolonged airway obstruction, inflammation and hyperresponsiveness. The aim this study was identify changes in the plasma proteome circulating hematopoietic progenitor cells associated LPR following an allergen challenge. Methods. Serial samples from asthmatics undergoing challenge were analyzed mass spectrometry immunosorbent assays. Peripheral blood...
<b>Background:</b> Alveolar type 2 epithelial cells (AEC2) secrete important surfactants and act as alveolar progenitor cells, but their involvement in lung extracellular matrix (ECM) production is not fully understood. The aim was to examine the capacity of AEC2 produce ECM contribution remodeling by using a new culture model. <b>Methods:</b> marker HT2-280 used isolate primary from healthy donor lungs end-stage COPD patients. Cryopreserved were then cultured non-diseased human...
Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease associated with heterogeneous and abnormal build-up of extracellular matrix (ECM) proteins increased fibroblast activity. We hypothesize that the alteration in material properties tissue one driving forces disease, where ECM stiffening has fundamental impact on cellular responses. Our aim to find correlation between structural alterations mechanisms pathology such as proliferation, synthesis mechanotransduction. Thinly sliced...
Decellularized tissues offer an excellent approach to study cell-ECM interaction in health and disease. We have used peripheral lung tissue from healthy individuals idiopathic pulmonary fibrosis (IPF) patients. IPF is characterized by excessive matrix production stiffening of the tissue. The aim was examine temporal changes original ECM scaffolds how these affect protein repopulating fibroblasts. Tissue slices (350µm) were decellularized using CHAPS generate biological scaffolds, density,...
<b>Background:</b> Protease activated receptor 2 (PAR2) is highly expressed on various cells. Although the well documented effects of PAR2 in innate immune responses to inflammation, knowledge pathogenesis chronic lung diseases limited. The objective was investigate role and its effect fibroblasts regarding changes phenotype profile mediator release fibroblast monoculture co-culture with mast <b>Methods:</b> experiments were performed 2D vitro cultures cell culture plastic a 3D ex vivo model...
Abstract Background The structure of alveoli is critical for normal lung function and when maintenance the underlying extracellular matrix (ECM) that forms these delicate structures fails, patients may develop emphysema or fibrosis via pathological processes are not fully understood. alveolar walls lined by epithelial cells (AEC), have so far been implicated in remodeling through signaling alters activity mesenchymal immune cells. We set out to examine capacity AEC produce ECM thereby...
Breathing exposes lung cells to continual mechanical stimuli that direct their functions. Therefore, developing in vitro model systems incorporate physiomimetic mechanics is urgent fully understand cell behavior. This study aims introduce a novel methodology combining simulates vivo breathing 3D culture platforms like recellularized human extracellular matrix (ECM) scaffolds and rat precision cut slices (PCLS). To this end, we have constructed device for controlled cyclic stretch, mimicking...
<b>Background:</b> Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate effect of on fibroblast activity migration cells. <b>Methods:</b> Primary distally-derived lung obtained from IPF patients healthy individuals cultured with LAD2 or stimulated cell proteases tryptase chymase for 72 hours. Human traditional culture plastics decellularized...