A5066064557- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Bone and Dental Protein Studies
- Ultrasound Imaging and Elastography
- Fungal and yeast genetics research
- Pickering emulsions and particle stabilization
- Photoacoustic and Ultrasonic Imaging
- Liver physiology and pathology
- Medical Imaging and Pathology Studies
- Intracranial Aneurysms: Treatment and Complications
- Ultrasound and Hyperthermia Applications
- Cardiovascular Health and Disease Prevention
- Cancer Cells and Metastasis
Delft University of Technology
2023-2025
Tumor initiation and progression are critically dependent on interaction of cancer cells with their cellular extracellular microenvironment. Alterations in the composition, integrity, mechanical properties matrix (ECM) dictate tumor processes including cell proliferation, migration, invasion. Also primary liver cancer, consisting hepatocellular carcinoma (HCC) cholangiocarcinoma (CCA), dysregulation environment by fibrosis desmoplasia is pertinent. Yet, exact changes occurring ECM remain...
Cholangiocarcinoma (CCA) is a type of liver cancer with an aggressive phenotype and dismal outcome in patients. The metastasis CCA cells to distant organs, commonly lung lymph nodes, drastically reduces overall survival. However, mechanistic insight how invades these metastatic sites still lacking. This partly because currently available models fail mimic the complexity tissue-specific environments for CCA. To create vitro model which interactions between epithelial tumor their surrounding...
Abstract The mechanical properties of cells are governed by the cytoskeleton, a dynamic network actin filaments, intermediate and microtubules. Understanding individual collective contributions these three different cytoskeletal elements is essential to elucidate how maintain integrity during deformation. Here we use custom single-cell rheometer identify distinct vimentin viscoelastic nonlinear elastic response uniaxial compression. We used mouse embryonic fibroblasts (MEFs) isolated from...
Cell migration is a fundamental process for life and highly dependent on the dynamical mechanical properties of cytoskeleton. Intensive physical biochemical crosstalk among actin, microtubules, intermediate filaments ensures their coordination to facilitate enable migration. In this review, we discuss different aspects that govern cell provide, each aspect, novel perspective by juxtaposing two complementary approaches biophysical study cytoskeletal crosstalk: live-cell studies (often...
Cell migration is a fundamental process for life and highly dependent on the dynamical mechanical properties of cytoskeleton. Intensive physical biochemical crosstalk between actin, microtubules, intermediate filaments ensures their coordination to facilitate enable migration. In this review we discuss different aspects that govern cell provide, each aspect, novel perspective by juxtaposing two complementary approaches biophysical study cytoskeletal crosstalk: live-cell studies (often...
ABSTRACT Drug transport from blood to extravascular tissue can locally be achieved by increasing the vascular permeability through ultrasound-activated microbubbles. However, mechanism remains unknown, including whether short and long cycles of ultrasound induce same onset rate, spatial distribution, amount increase. Accurate models are necessary for insights into so a microvessel-on-a-chip is developed with membrane-free space. Using these microvessels-on-a-chip, we show distinct...
Intermediate filaments (IFs) are a key component of the cytoskeleton, essential for regulating cell mechanics, maintaining nuclear integrity, positioning organelles, and modulating signaling. Unlike actin microtubules, IFs have slower dynamics, current insights into IF function primarily come from studies using long-term perturbations, such as protein depletion or mutation. Here, we present tools that allow rapid manipulation vimentin in whole cytoplasm within specific subcellular regions by...
Abstract Drug transport from blood to extravascular tissue can locally be achieved by increasing the vascular permeability through ultrasound‐activated microbubbles. However, mechanism remains unknown, including whether short and long cycles of ultrasound induce same onset rate, spatial distribution, amount increase. Accurate models are necessary for insights into so a microvessel‐on‐a‐chip is developed with membrane‐free space. Using these microvessels‐on‐a‐chip, distinct differences...