A5070146866- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Proteoglycans and glycosaminoglycans research
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Pulmonary Hypertension Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Asthma and respiratory diseases
- Glycosylation and Glycoproteins Research
- Inhalation and Respiratory Drug Delivery
- Transplantation: Methods and Outcomes
- Medical Imaging and Pathology Studies
- Mesenchymal stem cell research
- Tracheal and airway disorders
- Systemic Sclerosis and Related Diseases
- Electrospun Nanofibers in Biomedical Applications
- Peptidase Inhibition and Analysis
- Fibroblast Growth Factor Research
- Carbohydrate Chemistry and Synthesis
- Advanced Proteomics Techniques and Applications
- Respiratory Support and Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Connective tissue disorders research
- Mast cells and histamine
- Bioinformatics and Genomic Networks
- Cell Adhesion Molecules Research
Lund University
2016-2025
Lung Institute
2000-2015
AstraZeneca (Sweden)
2005-2013
University Hospital of Basel
2011
Karolinska Institutet
1998
An extensive remodelling process, referred to as cervical ripening, takes place in the tissue during pregnancy and labour. It is recognized softening dilation of canal, starts a slow process pregnancy, becoming rapid close partum. In this study we focus on cytokines possible mediators final remodelling. mRNA levels for interleukin (IL)-8, IL-6 granulocyte colony-stimulating factor (G-CSF) were upregulated ripe postpartum (n = 8) compared unripe state 9). Likewise, released cytokine...
The development of bleomycin-induced pulmonary fibrosis in rats was studied over a period 21 d after an intratracheal instillation bleomycin. expression three small proteoglycans (biglycan, decorin, and fibromodulin), collagen III TGF-beta 1 by RNA-transfer blot analysis. were also SDS-polyacrylamide gel electrophoresis Western blots. mRNA increased threefold already on day 3 remained elevated until 10. After the increase messages for biglycan steadily to reach maximum 10 bleomycin...
Abstract Direct conversion of human fibroblasts into mature and functional neurons, termed induced neurons ( iN s), was achieved for the first time 6 years ago. This technology offers a promising shortcut obtaining patient‐ disease‐specific disease modeling, drug screening, other biomedical applications. However, from adult donors do not reprogram as easily fetal donors, no current reprogramming approach is sufficiently efficient to allow use this using patient‐derived material large‐scale...
Rationale: Idiopathic pulmonary fibrosis (IPF) is a rare, irreversible, and progressive disease of the lungs. Common genetic variants, in addition to nongenetic factors, have been consistently associated with IPF. Rare variants identified by candidate gene, family-based, exome studies also reported associate However, extent which rare genome-wide, may contribute risk IPF remains unknown. Objectives: We used whole-genome sequencing investigate role on risk. Methods: As part Trans-Omics for...
Abstract Background Myofibroblasts, proposed as being derived from circulating fibrocytes, are considered to be important cells in thickening of the basement membrane patients with asthma. We have studied correlation tissue fibrocyte levels thickness and presence fibrocytes bronchoalveolar lavage fluid (BALF) steroid-naive mild asthma controls. Methods Patients (n = 9) were recruited divided into two categories based on whether or not fibroblast-like could established BALF. Non-asthmatic...
Airway remodeling in COPD includes reorganization of the extracellular matrix. Proteoglycans play a crucial role this process as regulators integrity Altered proteoglycan immunostaining has been demonstrated lungs and suggested to contribute pathogenesis. The major cell type responsible for production maintenance ECM constituents, such proteoglycans, are fibroblasts. Interestingly, it proposed that central airways alveolar lung parenchyma contain distinct fibroblast populations. This study...
Abstract Background Although mast cells are regarded as important regulators of inflammation and tissue remodelling, their role in cystic fibrosis (CF) idiopathic pulmonary (IPF) has remained less studied. This study investigates the densities phenotypes cell populations multiple lung compartments from patients with CF, IPF never smoking controls. Methods Small airways, vessels, parenchyma were subjected to detailed immunohistochemical analyses using lungs CF (20 regions; 5 patients), (21 7...
The extracellular matrix (ECM) is not just a three-dimensional scaffold that provides stable support for all cells in the lungs, but also an important component of chronic fibrotic airway, vascular, and interstitial diseases. It bioactive entity dynamically modulated during tissue homeostasis disease, controls structural immune cell functions drug responses, can release fragments have biological activity be used to monitor disease activity. There growing recognition importance considering...
Several interactions of cytokines with extracellular matrix molecules are mediated by proteoglycans, such as biglycan and decorin. Using surface plasmon resonance, we show for the first time that tumour necrosis factor-alpha (TNF-alpha) binds to both decorin K(d)s 0.81 microM 1.23 respectively, a binding was confirmed Scatchard plots using solid phase assay. Binding occurs preferentially via core protein, shown lower K(d)s, 0.26 respectively. There also dermatan sulphate, K(d) 10.53 microM....
Abstract We have modified a method (Gilles et al: Anal. Biochem. , 159:109–113, 1986) for measuring cell number, that is basec on the binding of crystal violet to nuclei and used it assay effects various glycosaminoglycans growth human lung fibroblasts. The procedure was by growing cells in microcultures (96 well microplates) amount adsorbed dye using microplate photometer after solubilisation with detergent. There linear relationship between absorbance number measured Coulter Counter. rapid...
Rhinovirus (RV)-induced chronic obstructive pulmonary disease (COPD) exacerbations exhibit TH(2)-like inflammation. We hypothesized that RV-infected bronchial epithelial cells (BEC) overproduce TH(2)-switching hub cytokine, thymic stromal lymphopoietin (TSLP) in COPD.Primary BEC from healthy (HBEC) and COPD donors (COPD-BEC) were grown 12-well plates, infected with RV16 (0.5-5 MOI) or stimulated agonists for either toll-like receptor (TLR) 3 (dsRNA, 0.1-10 μg/ml) RIG-I-like helicases...
Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem or other cell types through expression of specific combinations genes. This allows for the generation patient-specific lines that can serve as tools understanding disease pathogenesis, drug screens and, potentially, replacement therapies. Several cellular models neurological disorders based on induced (iPS cells) have been developed, and iPS-derived neurons being explored...