A5049250972- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Electrospun Nanofibers in Biomedical Applications
- Medical Imaging and Pathology Studies
- Pulmonary Hypertension Research and Treatments
- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inhalation and Respiratory Drug Delivery
- Asthma and respiratory diseases
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Reconstructive Surgery and Microvascular Techniques
- Peptidase Inhibition and Analysis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hydrogels: synthesis, properties, applications
- Air Quality and Health Impacts
- Proteins in Food Systems
- Mast cells and histamine
- Respiratory Support and Mechanisms
- Muscle Physiology and Disorders
- Congenital Diaphragmatic Hernia Studies
- COVID-19 Clinical Research Studies
- Bone Tissue Engineering Materials
- Connective tissue disorders research
Lund University
2014-2025
Congenital muscular dystrophy caused by laminin α2 chain deficiency (also known as MDC1A) is a severe and incapacitating disease, characterized massive muscle wasting. The ubiquitin-proteasome system plays major role in wasting we recently demonstrated that increased proteasomal activity feature of MDC1A. autophagy-lysosome pathway the other involved degradation proteins organelles within cell. However, it remains to be determined if dysregulated dystrophies, including Using dy3K/dy3K mouse...
Abstract Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin third component into the structure results in formation mesopores pore walls, which not seen if is excluded. The mesoporous explained by polyelectrolyte complexes between before crosslinking takes place. exhibit highly elastic properties withstanding compressions up to 60%. vitro biocompatibility cryogels evaluated fibroblasts from mouse cell...
Abstract Remodelling of the extracellular matrix is accomplished by altering balance between macromolecule production and degradation. However, it not well understood how cells new molecules degradation existing ones during tissue remodelling regeneration. In this study, we used decellularized lung scaffolds repopulated with allogenic fibroblasts cultured stable isotope labelled amino acids to quantify at a proteome-wide scale. Specific temporal dynamics different matrisome proteins were...
In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions. Scaffolds (decellularized tissue) derived from subpleural healthy IPF lungs were examined regarding biomechanical ECM composition proteins (the matrisome). repopulated with fibroblasts cultured under static stretch heavy isotope amino acids (SILAC), to examine newly synthesized over time. scaffolds characterized by...
Synchrotron-radiation nano-X-Ray Fluorescence (XRF) is a cutting-edge technique offering high-resolution insights into the elemental composition of biological tissues, shedding light on metabolic processes and element localization within cellular structures. In context Idiopathic Pulmonary Fibrosis (IPF), debilitating lung condition associated with respiratory complications reduced life expectancy, nano-XRF presents promising avenue for understanding disease's intricate pathology. Our...
Background In this study, we combine proteomics with functional parameters and imaging to examine potential biomarkers that may identify patients at-risk of developing persistent lung sequelae following coronavirus disease 2019 (COVID-19). Methods We performed multiplex profiling serum collected clinical data from previously intensive care unit (ICU)-treated COVID-19 (n=43) at four ten months post hospitalization. Results Four discharge, demonstrated lower % predicted forced vital capacity...
Idiopathic pulmonary fibrosis (IPF) is characterized by an aberrant repair response with uncontrolled turnover of extracellular matrix involving mesenchymal cell phenotypes, where lung resident stem cells (LRMSC) have been supposed to important role. However, the contribution LRMSC in not fully understood, and role IPF remains be elucidated. Here, we performed transcriptomic functional analyses on isolated from control patients (CON). Both over-representation gene set enrichment indicated...
microRNAs (miRNAs) are widespread regulators of gene expression, but little is known their potential roles in congenital muscular dystrophy type 1A (MDC1A). MDC1A a severe form caused by mutations the encoding laminin α2 chain. To gain insight into pathophysiological miRNAs associated with pathology, chain-deficient mice were evaluated quantitative PCR. We demonstrate that expression muscle-specific miR-1, miR-133a, and miR-206 deregulated muscle. Furthermore, miR-223 miR-21, immune cell...
Acute Respiratory Distress Syndrome is one of the more common fatal complications in COVID-19, characterized by a highly aberrant inflammatory response. Pre-clinical models to study effect cell therapy and anti-inflammatory treatments have not comprehensively reproduced disease due its high complexity. This work presents novel physiomimetic vitro model for using lung extracellular matrix-derived hydrogels organ-on-a-chip devices. Monolayres primary alveolar epithelial cells were cultured on...
Lung-resident mesenchymal stem cells (LR-MSC) are thought to participate in idiopathic pulmonary fibrosis (IPF) by differentiating into myofibroblasts. On the other hand, LR-MSC IPF patients present senescence-related features. It is unclear how they respond a profibrotic environment. Here, we investigated response of isolated from and control (CON) patients. were inoculated mice 48 h after bleomycin (BLM) instillation analyze their contribution lung damage. In vitro, exposed TGFβ. Mice with...
Abstract In this study a new way to produce supermacroporous protein structures was investigated. Enzyme‐mediated crosslinking of gelatin or casein performed in partly frozen state, which yielded stable, protein‐based cryogels. The reaction kinetics for the formation cryogels were found be fairly slow, most likely due low temperature (−12 °C) used an increased viscosity owing cryo‐concentration taking place. produced characterized with regards their physical properties and vitro degradation....
Accurate fluid pressure in the fetal lung is critical for its development, especially at beginning of saccular stage when alveolar epithelial type 1 (AT1) and 2 (AT2) cells differentiate from progenitors. Despite our growing understanding role physical forces molecular mechanisms that regulate transduction mechanical stretch to differentiation remain elusive. To simulate distension, we optimized both an ex vivo model with precision cut slices tracheal occlusion. Increased tension showed...
Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate effect of on fibroblast activity migration cells. Lung from IPF patients healthy individuals co-cultured with LAD2 or stimulated proteases tryptase chymase. Human lung cultured cell culture plastic plates decellularized human tissue (scaffolds) create a more physiological milieu by...
Healthy regeneration of tissue relies on a well-orchestrated release growth factors. Herein, we show the use synthetic glycosaminoglycans for controlled binding and factors to induce desired cellular response. First, screened with interest determine k
The field of tissue engineering has a growing need for suitable scaffold materials to become attractive as clinical therapy. To use completely autologous construct repair damaged or diseased is an appealing thought. As model system, two types scaffolds were prepared from biological fluids: blood and plasma. formed macroporous structure with elastic mechanical properties that further evaluated myoblast cell line (C2C12) cultivation transplantation into mouse skeletal muscle. cells found...
Background Pulmonary hypoplasia, characterized by incomplete alveolar development, remains a major cause of mortality and morbidity in congenital diaphragmatic hernia. Recently demonstrated to differentiate from common bipotent progenitor during the two cell types that line alveoli type 1 2 cells have shown alter their relative ratio hernia lungs. Objective We used nitrofen/bisdiamine mouse model induce accurately assess status epithelial differentiation relation progenitors. Study design...
Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic lung disease with an unmet need of biomarkers that can aid in the diagnostic and prognostic assessment response to treatment. In this two-part explorative proteomic study, we demonstrate how proteins associated tissue remodeling, inflammation chemotaxis such as MMP7, CXCL13 CCL19 are released aberrant extracellular matrix (ECM) IPF lung. We used novel ex vivo model where decellularized from patients healthy donors were repopulated...
Mesenchymal stromal cell (MSC)-based therapies for inflammatory diseases rely mainly on the paracrine ability to modulate activity of macrophages. Despite recent advances, there is scarce information regarding changes secretome content attributed physiomimetic cultures and, especially, how influence macrophage therapy. hLMSCs from human donors were cultured devices developed in house that enabled lung-mimetic strain. hLMSC was analyzed typical cytokines, chemokines and growth factors. RNA...
Breathing exposes lung cells to continual mechanical stimuli, which is part of the microenvironmental signals directing cellular functions together with extracellular matrix (ECM). Therefore, developing systems that incorporate both stimuli urgent fully understand cell behavior. This study aims introduce a novel in vitro culture methodology combining cyclic stretch simulates vivo breathing 3D platforms form decellularized slices (DLS) and precision cut (PCLS). To this end, we have...