A5055459164- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- Congenital Anomalies and Fetal Surgery
- Congenital Heart Disease Studies
- Respiratory Support and Mechanisms
- Neuroscience of respiration and sleep
- Tracheal and airway disorders
- Hernia repair and management
- Mesenchymal stem cell research
- Blood transfusion and management
- Quantum Dots Synthesis And Properties
- Gold and Silver Nanoparticles Synthesis and Applications
- Bone fractures and treatments
- Peroxisome Proliferator-Activated Receptors
- Infant Nutrition and Health
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Esophageal and GI Pathology
- Fetal and Pediatric Neurological Disorders
- Renal and related cancers
- Ultrasound in Clinical Applications
- Cardiac Imaging and Diagnostics
- Perfectionism, Procrastination, Anxiety Studies
- High Altitude and Hypoxia
- Cardiovascular Issues in Pregnancy
- Cultural and Mythological Studies
Clínica Alemana
2012-2025
KU Leuven
2014-2025
Universidad del Desarrollo
2015-2024
Universidad Católica de Cuenca
2023
Organ Recovery Systems (Belgium)
2014-2019
Hudson Institute
2017
Hospital Padre Hurtado
2009-2014
University of Chile
2009
Ponce Health Sciences University
2009
University of Washington
2007
<h3>Introduction</h3> The management of congenital diaphragmatic hernia (DH) would benefit from an antenatal medical therapy, which addresses both lung hypoplasia and persistent pulmonary hypertension. We aimed at evaluating the effects sildenafil in fetal rabbit model for DH. <h3>Methods</h3> performed a dose-finding study to achieve therapeutic plasmatic concentrations without toxicity following maternal administration. Subsequently, DH fetuses were randomly exposed transplacental placebo...
<b><i>Background:</i></b> Caffeine is a commonly used drug for apnea of prematurity. It may, however, also have beneficial effect on bronchopulmonary dysplasia (BPD), which the most common complication extreme preterm birth. <b><i>Objectives:</i></b> To study inflammatory, structural and functional effects caffeine in an animal model BPD. <b><i>Methods:</i></b> Preterm New Zealand-Dendermonde rabbits (gestational day 28;...
Congenital diaphragmatic hernia (CDH) results in lung hypoplasia. In severe cases, tracheal occlusion (TO) can be offered to promote growth. However the benefit is limited, and novel treatments are required supplement TO. Vascular endothelial growth factor (VEGF) downregulated animal models of CDH could a therapeutic target, but its role human not known. To investigate whether VEGF supplementation suitable treatment for CDH-associated pathology. Fetal lungs from patients were used determine...
Abstract Background Bronchopulmonary dysplasia continues to cause important respiratory morbidity throughout life, and new therapies are needed. The common denominator of all BPD cases is preterm birth, however most preclinical research in this area focusses on the effect hyperoxia or mechanical ventilation. In study we investigated if how prematurity affects lung structure function neonatal rabbits. Methods Pups were delivered either day 28 31. For each gestational age a group pups was...
Bronchopulmonary dysplasia (BPD) is caused by preterm neonatal lung injury and results in oxygen dependency pulmonary hypertension. Current clinical management fails to reduce the incidence of BPD, which calls for novel therapies. Fetal rabbits have a development that mimics humans can be used as translational model test treatment options. In rabbits, exposure hyperoxia leads parenchymal changes, yet vascular damage has not been studied this model. study we document early functional...
To date, most nanomedical studies rely on the use of immune-deficient mice in which contribution immune system applied therapy is ignored. Here, degradation silver nanoparticles (Ag NPs) exploited as a means to treat subcutaneous tumor models mice. investigate impact system, same cell type (KLN 205 murine squamous carcinoma) used xenograft model NOD SCIDγ and syngeneic immune-competent DBA/2 The Ag NPs are screened for their cytotoxicity various cancer lines, indicating...
Reproducible and unbiased methods to quantify alveolar structure are important for research on many lung diseases. However, manually estimating through stereology is time consuming inter-observer variability high. The objective of this work was develop validate a fast, reproducible accurate (semi-)automatic alternative. A FIJI-macro designed that automatically segments images binary masks, counts the number test points falling tissue intersections air-tissue interface with set lines. Manual...
The neonatal management of preterm born infants often results in damage to the developing lung and subsequent morbidity, referred as bronchopulmonary dysplasia (BPD). Animal models may help understanding molecular processes involved this condition define therapeutic targets. Our goal was identify pathways using earlier described rabbit model hyperoxia induced lung-injury. Transcriptome analysis by mRNA-sequencing performed on lungs from pups at day 28 gestation (term: 31 days) kept (95% O2)...
The administration of supplemental oxygen to treat ventilatory insufficiency may lead the formation reactive species and subsequent tissue damage. Cytochrome P4501A1 (CYP1A1) can modulate hyperoxic lung injury by a currently unknown mechanism. Our objective was evaluate effect omeprazole on induction CYP1A1 its influence in an established preterm rabbit model. Omeprazole administered either (1) directly fetus, (2) mother or (3) after birth pups different doses (2–10 20 mg/kg). Controls were...
Recent clinical trials in newborns have successfully used surfactant as a drug carrier for an active compound, to minimize systemic exposure. To investigate the translational potential of surfactant-compound mixtures and other local therapeutics, relevant animal model is required which intratracheal administration maximal deposition technically possible well tolerated. Preterm rabbit pups (born at 28 days gestation) were exposed either hyperoxia or normoxia randomized receive daily...
We aimed to assess in vivo changes lung and liver volumes fetuses with isolated congenital diaphragmatic hernia, either expectantly managed or treated utero.This is a secondary analysis of prospectively collected data at two fetal therapy centers. used archived magnetic resonance images taken ≥7 days apart, creating paired observations 20 cases, 41 second prior balloon reversal 64 after removal. measured observed expected total volume (O/E TFLV) liver-to-thoracic ratio. calculated as...
<b><i>Background:</i></b> Bronchopulmonary dysplasia (BPD) is a chronic lung disease that affects extremely preterm infants and remains – despite improvements in neonatal intensive care major cause of mortality morbidity. Cell-therapeutic strategies employing mesenchymal stem cells (MSC) have been shown to modulate development BPD models. <b><i>Objective:</i></b> Herein, we evaluate the potential human amniotic fluid (hAF)-SC hAF-SC with...
Objective: To evaluate the effect of combining antenatal sildenafil with fetal tracheal occlusion (TO) in rabbits surgically induced congenital diaphragmatic hernia (CDH). Background: Although administration rescues vascular abnormalities lungs CDH, it only partially improves airway morphometry. We hypothesized that we could additionally stimulate lung growth by this medical treatment TO. Methods: CDH was created on gestational day (GD)23 (n=54). Does were randomized to receive either 10...
Background Pulmonary hypoplasia, characterized by incomplete alveolar development, remains a major cause of mortality and morbidity in congenital diaphragmatic hernia. Recently demonstrated to differentiate from common bipotent progenitor during the two cell types that line alveoli type 1 2 cells have shown alter their relative ratio hernia lungs. Objective We used nitrofen/bisdiamine mouse model induce accurately assess status epithelial differentiation relation progenitors. Study design...
Abstract Rationale Congenital diaphragmatic hernia (CDH) results in lung hypoplasia. In severe cases, tracheal occlusion (TO) can be offered to promote growth. However the benefit is limited, and novel treatments are required supplement TO. Vascular endothelial growth factor (VEGF) downregulated animal models of CDH could a therapeutic target, but its role human not known. Objectives To investigate whether VEGF supplementation suitable treatment for CDH-associated pathology. Methods Fetal...
To evaluate novel sealing techniques for their biocompatibility and capacity of iatrogenic fetal membrane defects in a pregnant rabbit model.At day 23 gestation (term = d31), standardized fetoscopy was performed through 14G cannula. The resulting defect closed with condensed collagen, collagen fibrinogen, Tissuepatch, Duraseal, or conventional plug (Lyostypt) as reference. At d30, the fetuses were harvested full thickness samples analyzed. study consisted 2 consecutive parts: (1) testing by...
The objective of this study is to evaluate whether assessment pulmonary vascular reactivity in response maternal hyperoxygenation using fractional moving blood volume (FMBV) associated with lesser variability between individual measurements than what observed direct Doppler measurements.Forty-five were performed 15 singleton fetuses normal lung development at three time points the latter half pregnancy (range: 25.9-36.7 weeks). We further evaluated five severe congenital diaphragmatic...
Continuous improvements in perinatal care have allowed the survival of increasingly more prematurely born infants. The establishment respiration an extremely immature yet still developing lung results chronic injury with significant mortality and morbidity. We experimentally evaluated a novel medical strategy to prevent hyperoxia-induced by prenatal rosiglitazone.Pregnant rabbits were injected saline or rosiglitazone (3 mg/kg) 48 24 h prior preterm delivery at 28 days gestation (term = 31...