Jessica Allen-Hyttinen
A5013208052- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Respiratory viral infections research
- Hernia repair and management
- Infant Nutrition and Health
- Long-Term Effects of COVID-19
- Biosensors and Analytical Detection
- IL-33, ST2, and ILC Pathways
- SARS-CoV-2 detection and testing
- Congenital Anomalies and Fetal Surgery
- Pediatric health and respiratory diseases
- Ethics and Legal Issues in Pediatric Healthcare
- Inflammasome and immune disorders
University College London
2021-2025
It is not fully understood why COVID-19 typically milder in children
The COVID-19 pandemic is an ongoing global health threat, yet our understanding of the dynamics early cellular responses to this disease remains limited
Studies of human lung development have focused on epithelial and mesenchymal cell types function, but much less is known about the developing immune cells, even though airways are a major site mucosal immunity after birth. An unanswered question whether tissue-resident cells play role in shaping tissue as it develops utero. Here, we profiled embryonic fetal using scRNA-seq, smFISH, immunohistochemistry. At stage, observed an early wave innate including lymphoid natural killer myeloid lineage...
Congenital diaphragmatic hernia (CDH) results in lung hypoplasia. In severe cases, tracheal occlusion (TO) can be offered to promote growth. However the benefit is limited, and novel treatments are required supplement TO. Vascular endothelial growth factor (VEGF) downregulated animal models of CDH could a therapeutic target, but its role human not known. To investigate whether VEGF supplementation suitable treatment for CDH-associated pathology. Fetal lungs from patients were used determine...
Abstract The COVID-19 pandemic is an ongoing global health threat, yet our understanding of the cellular disease dynamics remains limited. In unique human challenge study we used single cell genomics nasopharyngeal swabs and blood to temporally resolve abortive, transient sustained infections in 16 seronegative individuals challenged with preAlpha-SARS-CoV-2. Our analyses revealed rapid changes type proportions dozens highly dynamic response states epithelial immune cells associated specific...
Abstract While a substantial proportion of adults infected with SARS-CoV-2 progress to develop severe disease, children rarely manifest respiratory complications. Therefore, understanding differences in the local and systemic response infection between may provide important clues about pathogenesis infection. To address this, we first generated healthy reference multi-omics single cell data set from (n=30) whom have profiled triple matched samples: nasal tracheal brushings PBMCs, where track...
Abstract Purpose This paper aims to build upon previous work definitively establish in vitro models of murine pseudoglandular stage lung development. These can be easily translated human fetal samples allow the investigation development physiologic and pathologic conditions. Methods Lungs were harvested from mouse embryos at E12.5 cultured three different settings, i.e., whole culture, mesenchyme-free epithelium organoid culture. For extracted lungs embedded Matrigel incubated on permeable...
Abstract Rationale Congenital diaphragmatic hernia (CDH) results in lung hypoplasia. In severe cases, tracheal occlusion (TO) can be offered to promote growth. However the benefit is limited, and novel treatments are required supplement TO. Vascular endothelial growth factor (VEGF) downregulated animal models of CDH could a therapeutic target, but its role human not known. Objectives To investigate whether VEGF supplementation suitable treatment for CDH-associated pathology. Methods Fetal...
<h3>Introduction and Objectives</h3> Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the current disease 2019 (COVID-19) pandemic. Whilst a substantial proportion of adults infected with SARS-CoV-2 progress to develop severe disease, children rarely manifest complications. However, underlying mechanism behind this disparity remains largely unknown. Understanding differences in local systemic response infection at single cell resolution between may offer key...
<b>Background:</b> Children typically present with milder coronavirus disease 2019 (COVID-19) severity compared to adults. The molecular basis of the differences in COVID-19 progression between children and adults remains be elucidated. Here we investigated age-specific airway mucosal immunology as well systemic immune responses severe acute respiratory syndrome 2 (SARS-CoV-2) infection. <b>Methods:</b> We analysed matched nasal, tracheal blood samples from healthy patients infancy adulthood...
Despite the rapid development and rollout of first-generation vaccines, COVID-19 remains an ongoing global health concern. Whilst single cell transcriptional studies have provided deep biological insights, limited longitudinal sampling has made temporal inferences difficult. The Human Challenge Study offers a unique opportunity to study these cellular dynamics, from pre-infection recovery, in controlled safe environment. Matched nasopharyngeal swabs peripheral blood samples were collected at...
Abstract During human development, lungs develop their roles of gas exchange and barrier function. Recent single cell studies have focused on epithelial mesenchymal types, but much less is known about the developing lung immune cells, although airways are a major site mucosal immunity after birth. An open question whether tissue-resident cells play role in shaping tissue as it develops utero . In order to address this, we profiled using scRNAseq, smFISH immunohistochemistry. At embryonic...