A5032662640- Malaria Research and Control
- Tuberculosis Research and Epidemiology
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Complement system in diseases
- Mycobacterium research and diagnosis
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Invertebrate Immune Response Mechanisms
- Hepatitis B Virus Studies
- interferon and immune responses
- Aquaculture disease management and microbiota
- Vector-borne infectious diseases
- Immunotherapy and Immune Responses
- Immune responses and vaccinations
- Lipid Membrane Structure and Behavior
- Hepatitis C virus research
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
District University of Bogotá
2020-2025
hVIVO (United Kingdom)
2023
Fundación Instituto de Inmunología de Colombia
2012-2022
Mount Sinai Medical Center
2021
Instituto Politécnico Nacional
2020
Universidad del Rosario
2009-2019
Universidad Nacional de Colombia
2000-2014
Universidad Colegio Mayor de Cundinamarca
2012
To determine amino acid sequences of the Plasmodium falciparum MSP‐1 protein that interact with red blood cell membranes in a specific receptor‐ligand interaction, 78 sequential peptides, 20 acids long and spanning entire length molecule, were synthesized analysed binding assay developed for this purpose. Results show peptides based on conserved dimorphic regions MSP‐1, human cells (RBCs). This interaction occurs predominantly contained within proteolytic fragments 83 kDa, 38 33 kDa 19 kDa....
Background/Objective: Finding new targets to attenuate Mycobacterium tuberculosis (Mtb) is key in the development of TB vaccines. In this context, plasma membrane P-type ATPases are relevant for mycobacterial homeostasis and virulence. work, we investigate role copper-transporting ATPase CtpA Mtb Methods: The impact deletion on Mtb's capacity overcome redox stress proliferate mouse alveolar macrophages (MH-S) was evaluated, as well its effect immunogenicity. Moreover, influence pathogenicity...
Solid experimental evidence indicates that EBA-175 is used as a ligand by the Plasmodium falciparum merozoite to bind human RBC, via different binding processing fragments. Using synthetic peptides and specific receptor-ligand interaction methodology, we have identified 6 high-activity sequences from CAMP strain; peptide 1758 (KSYGTPDNIDKNMSLIHKHN), located in so-called region I for which no activity has been reported before, 1779 (NIDRIYDKNLLMIKEHILAI) 1783 (HRNKKNDKLYRDEWWKVIKK), II,...
Rational design of a malaria vaccine may result from the structure–immunogenicity relationship analogues peptide 1585, protein sequence located at N-terminal end P. falciparum 42-kDa merozoite surface protein-1. Altering few amino acids in 1585 (left) induces immunogenicity and protectivity, changes 3D structure, as shown for one synthesized (right).
The mycobacterial cell envelope has been implicated in the pathogenicity of tuberculosis and therefore a prime target for identification characterization surface proteins with potential application drug vaccine development. In this study, genome Mycobacterium H37Rv was screened using Machine Learning tools that included feature-based predictors, general localizers transmembrane topology predictors to identify are potentially secreted M. tuberculosis, or extracellular milieu through different...
Abstract: Plasmodium falciparum merozoite membrane surface antigen 2 (MSA 2) has been associated with the development of protective immunity against malaria. MSA antibodies were able to inhibit in vitro invasion. In our search for experimental evidence concerning participation invasion, 40 peptides synthesized according sequences reported CAMP and FC27 prototype strains. These purified, 125 I‐radiolabeled tested their ability bind erythrocytes. Two synthetic high specific binding human...
Abstract Background The computational prediction of mycobacterial proteins' subcellular localization is key importance for proteome annotation and the identification new drug targets vaccine candidates. Several classifiers have been developed over past few years, which comprised both general feature-based classifiers. Here, we validated ability different bioinformatics approaches, through use SignalP 2.0, TatP 1.0, LipoP Phobius, PA-SUB 2.5, PSORTb v.2.0.4 Gpos-PLoc, to predict secreted...
To date, the function of many hypothetical membrane proteins Mycobacterium tuberculosis is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, biological activity peptides derived from protein Rv0679c M. was assessed. Transcription gene studied 26 spp. Strains. Antibodies raised against putative B-cell epitopes were used Western blot immunoelectron microscopy assays. Synthetic spanning entire length tested for ability to bind...
This work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to mammalian cell entry family (mce) which is thought have important functions in disease's pathogenesis. The protein characterized silico; its presence on mycobacterial surface confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified assays with (125)I; their ability inhibit two lines (U937 alveolar macrophages and A549 epithelial...
Virulence and immunity are still poorly understood in Mycobacterium tuberculosis. The H37Rv M. tuberculosis laboratory strain genome has been completely sequenced, this along with proteomic technology represent powerful tools contributing toward studying the biology of target cell interaction a facultative bacillus designing new strategies for controlling Rv2004c is putative protein that could have specific mycobacterial functions. This study revealed encoding gene present all mycobacterium...
The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in complex (as assayed by PCR) and transcribed determined RT-PCR) M. H37Rv, H37Ra, bovis BCG, africanum strains. Rabbits immunized with synthetic polymer peptides from this produced antibodies specifically recognizing a 25-kDa band mycobacterial sonicate. U937 A549 cells were used binding assays involving 20-amino-acid-long covering whole sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific to...
Abstract Receptor–ligand interactions between synthetic peptides and normal human erythrocytes were studied to determine Plasmodium falciparum merozoite surface protein‐3 (MSP‐3) FC27 strain regions that specifically bind membrane receptors on erythrocytes. Three MSP‐3 protein high activity binding (HABPs) identified; their became saturable, had nanomolar affinity constants, sensitive being treated with neuraminidase trypsin but resistant chymotrypsin treatment. All of them recognized 45‐,...