A5023232987- Muscle Physiology and Disorders
- Cellular transport and secretion
- Extracellular vesicles in disease
- COVID-19 Impact on Reproduction
- Advanced Sensor and Energy Harvesting Materials
- MicroRNA in disease regulation
- Muscle activation and electromyography studies
- RNA Interference and Gene Delivery
- Hernia repair and management
- Congenital Diaphragmatic Hernia Studies
- Erythrocyte Function and Pathophysiology
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Pregnancy and preeclampsia studies
- Biotin and Related Studies
- Lipid Membrane Structure and Behavior
- Cancer Cells and Metastasis
- Contact Dermatitis and Allergies
- Skin and Cellular Biology Research
- Neonatal Respiratory Health Research
- Advanced biosensing and bioanalysis techniques
- Pregnancy and Medication Impact
- Knee injuries and reconstruction techniques
University College London
2019-2025
Great Ormond Street Hospital
2020-2025
MRC Laboratory for Molecular Cell Biology
2021-2022
Great Ormond Street Hospital for Children NHS Foundation Trust
2020-2021
Abstract Three-dimensional hydrogel-based organ-like cultures can be applied to study development, regeneration, and disease in vitro. However, the control of engineered hydrogel composition, mechanical properties geometrical constraints tends restricted initial time fabrication. Modulation characteristics over according culture evolution is often not possible. Here, we overcome these limitations by developing a hydrogel-in-hydrogel live bioprinting approach that enables dynamic fabrication...
Congenital diaphragmatic hernia (CDH) results in lung hypoplasia. In severe cases, tracheal occlusion (TO) can be offered to promote growth. However the benefit is limited, and novel treatments are required supplement TO. Vascular endothelial growth factor (VEGF) downregulated animal models of CDH could a therapeutic target, but its role human not known. To investigate whether VEGF supplementation suitable treatment for CDH-associated pathology. Fetal lungs from patients were used determine...
Abstract During the last decade, multiple clinical trials for Duchenne muscular dystrophy (DMD) have focused on induction of dystrophin expression using different strategies. Many these reported a clear increase in protein following treatment. However, low levels induced raised questions its functionality. In our present study, an unbiased, high-throughput digital image analysis platform, we assessed markers regeneration and associated via immunofluorescent whole muscle sections 25 DMD boys...
Background Pregnant women have been identified as a potentially at‐risk group concerning COVID‐19 infection, but little is known regarding the susceptibility of fetus to infection. Co‐expression ACE2 and TMPRSS2 has prerequisite for expression across different tissues vary between children adults. However, these proteins in unknown. Methods We performed retrospective analysis single cell data repository. The were then validated at both gene protein level by performing RT‐qPCR two‐colour...
Abstract Duchenne muscular dystrophy (DMD) is an incurable disease caused by out-of-frame DMD gene deletions while in frame lead to the milder Becker (BMD). In last decade several antisense oligonucleotides drugs have been developed induce a partially functional internally deleted dystrophin, similar that produced BMD, and expected ameliorate course. The pattern of dystrophin expression functionality dystrophinopathy patients variable due multiple factors, such as molecular its distribution....
Abstract Rationale Congenital diaphragmatic hernia (CDH) results in lung hypoplasia. In severe cases, tracheal occlusion (TO) can be offered to promote growth. However the benefit is limited, and novel treatments are required supplement TO. Vascular endothelial growth factor (VEGF) downregulated animal models of CDH could a therapeutic target, but its role human not known. Objectives To investigate whether VEGF supplementation suitable treatment for CDH-associated pathology. Methods Fetal...
Abstract The primary molecular endpoint for many Duchenne muscular dystrophy (DMD) clinical trials is the induction, or increase in production, of dystrophin protein striated muscle. For accurate analysis, it essential to have reliable, robust and objective quantification methodologies capable detecting subtle changes expression. In this work, we present further development optimisation an automated, digital, high-throughput script quantitative analysis multiplexed immunofluorescent (IF)...
In vertebrate cells, the Golgi functional subunits, mini-stacks, are linked into a tri-dimensional network. How this "ribbon" architecture relates to functions remains unclear. Are all connections between mini-stacks equal? Is local structure of ribbon importance? These difficult questions address, without quantifiable readout output ribbon-embedded mini-stacks. Endothelial cells produce secretory granules, Weibel-Palade bodies (WPB), whose von Willebrand Factor (VWF) cargo is central...
Aim: To perform cross-sectional and longitudinal miRNA profiling in plasma from Duchenne muscular dystrophy (DMD) subjects find non-invasive biomarkers DMD. Subjects/materials & methods: Plasma was collected 14 age sex matched controls 46 DMD subjects. Free-circulating extracellular vesicle (EV)-derived expression measured by RT-qPCR. Results: EVs derived miR-29c-3p miR-133a-3p are dysregulated EV-derived reduced undergoing daily corticosteroid treatment. miR-1-3p miR-122-5p longitudinally...
Background: While pregnant women have been identified as a potentially at-risk group concerning COVID-19 infection, little is known regarding the susceptibility of fetus to infection. Co-expression ACE2 and TMPRSS2 has pre-requisite for expression across different tissues vary between children adults. However, these proteins in unknown. Methods: We performed retrospective analysis single cell data repositories. This was then validated at both gene protein level by performing qRT-PCR...
Background: While pregnant women have been identified as a potentially at-risk group concerning COVID-19 infection, little is known regarding the susceptibility of fetus to infection. Co-expression ACE2 and TMPRSS2 has pre-requisite for expression across different tissues vary between children adults. However, these proteins in unknown.Methods: We performed retrospective analysis single cell data repositories. This was then validated at both gene protein level by performing qRT-PCR...
Abstract Vertebrate cells display a specific Golgi apparatus architecture, known as the “ribbon”, where functional subunits, mini-stacks, are linked into tridimensional network. The importance of ribbon architecture is underscored by evidence its disruption in host diseases, but just how it relates to biological functions remains unclear. Are all connections between mini-stacks functionally equal? Is local structure importance? These difficult questions address, due lack secretory cargo...