A5054216663- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- Corneal Surgery and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Ocular Disorders and Treatments
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Marine Invertebrate Physiology and Ecology
- Viral-associated cancers and disorders
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Hedgehog Signaling Pathway Studies
- Corneal surgery and disorders
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Retinal Development and Disorders
- Herpesvirus Infections and Treatments
- Developmental Biology and Gene Regulation
- Photoreceptor and optogenetics research
- Medicinal plant effects and applications
- Virus-based gene therapy research
- Retinal Diseases and Treatments
Città della Speranza Foundation
2021-2023
University of Padua
2016-2021
Veneto Institute of Molecular Medicine
2020
Abstract Three-dimensional hydrogel-based organ-like cultures can be applied to study development, regeneration, and disease in vitro. However, the control of engineered hydrogel composition, mechanical properties geometrical constraints tends restricted initial time fabrication. Modulation characteristics over according culture evolution is often not possible. Here, we overcome these limitations by developing a hydrogel-in-hydrogel live bioprinting approach that enables dynamic fabrication...
We have previously shown that clusters of guanine quadruplex (G4) structures can form in the human herpes simplex-1 (HSV-1) genome. Here we used immunofluorescence and immune-electron microscopy with a G4-specific monoclonal antibody to visualize G4 HSV-1 infected cells. found formation localization within cells was virus cycle dependent: viral G4s peaked at time DNA replication cell nucleus, moved nuclear membrane egress were later immature virions released from nucleus. Colocalization...
In this work, the authors presented techniques and preliminary results at 6 months of a randomized controlled trial ( NCT02117999 ) comparing novel transepithelial corneal cross-linking protocol using iontophoresis with Dresden for treatment progressive keratoconus. At months, there was significant average improvement an flattening maximum simulated keratometry reading of<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mn...
Abstract Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome is a rare autosomal dominant disease caused by heterozygous mutations in the p63 gene and characterized limb defects, orofacial clefting, ectodermal dysplasia, ocular defects. Patients develop progressive total bilateral limbal stem cell deficiency, which eventually results corneal blindness. Medical surgical treatments are ineffective of limited benefit. Oral mucosa epithelial cells (OMESCs) represent an alternative source...
Abstract Skeletal muscle decellularization allows the generation of natural scaffolds that retain extracellular matrix (ECM) mechanical integrity, biological activity, and three-dimensional (3D) architecture native tissue. Recent reports showed in vivo implantation decellularized muscles supports regeneration volumetric loss models, including nervous system neuromuscular junctional homing. Since plays pivotal roles during skeletal tissue homeostasis, support reinnervation is a crucial aspect...
Abstract Ectrodactyly-ectodermal dysplasia-clefting (EEC) syndrome is a rare autosomal dominant disease caused by mutations in the p63 gene. To date, approximately 40 different have been identified, all heterozygous. No definitive treatments are available to counteract and resolve progressive corneal degeneration due premature aging of limbal epithelial stem cells. Here, we describe unique case young female patient, aged 18 years, with EEC dysfunction, who was, surprisingly, homozygous for...
Purpose To identify novel mutations in the retinitis pigmentosa GTPase regulator ( RPGR) gene and 2 RP2) underlying X-linked (XLRP) assess genotype-phenotype correlations. Methods The patient cohort, consisting of 13 individuals from 3 unrelated XLRP families, underwent comprehensive ophthalmologic examination. open reading frames RPGR RP2 were analyzed with Sanger sequencing each patient. identified genetic variants defined as or polymorphisms on basis their pathological effect. Results We...
Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome is a rare monogenic disease with autosomal dominant inheritance caused by mutations in the TP63 gene, leading to progressive corneal keratinocyte loss, limbal stem cell deficiency (LSCD), and eventually blindness. Currently, there no treatment available cure or slow down loss. Human oral mucosal epithelial cells (hOMESCs), which are mixed population of precursor cells, used as source autologous tissue for bilateral LSCD. However,...
Abstract Human neuromuscular organoids (NMOs) derived from induced pluripotent stem cells (hiPSCs) hold a great potential to study (dys)functional human skeletal muscle (SkM) in vitro. The three-dimensional (3D) self-assembly of NMOs leads the generation spheroids, whose 3D organization cannot be controlled. Indeed, proper development, maturation and function innervated SkM require well-defined multiscale tissue-specific extracellular matrix (ECM) context. We hypothesized that structural...
Abstract Tissue architecture is a driving force for morphogenetic processes during development as well several physiological and regenerative responses. Far from being passive static environment, tissue highly dynamic. Hydrogel technology reproduces in vitro geometrical mechanical constrains that control the three-dimensional self-organization of (3D) organoids organ-like cultures. This restricted to initial culture conditions cannot be adapted dynamic morphological changes complex 3D...
Decellularized skeletal muscle (dSkM) constructs have received much attention in recent years due to the versatility of their applications vitro. In search adequate vitro models tissue, dSkM offers great advantages terms preservation native-tissue complexity, including three-dimensional organization, presence residual signaling molecules within construct, and myogenic neurotrophic abilities. Here, we attempted develop a 3D model neuromuscular tissue. To do so, repopulated rat with human...