A5056710864- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Tissue Engineering and Regenerative Medicine
- Nerve injury and regeneration
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- Biochemical effects in animals
- MicroRNA in disease regulation
- Muscle Physiology and Disorders
- Single-cell and spatial transcriptomics
- Connexins and lens biology
- Organ Transplantation Techniques and Outcomes
- Multiple Sclerosis Research Studies
- Biomedical Ethics and Regulation
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
- Heat shock proteins research
- Cancer Immunotherapy and Biomarkers
Veneto Institute of Molecular Medicine
2020-2024
University of Padua
2020-2024
Institute of Molecular Medicine
2023
Consorzio per la Ricerca Sanitaria
2021
Lund University
2016-2020
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2020
Vita-Salute San Raffaele University
2014-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
Institute of Neuroimmunology of the Slovak Academy of Sciences
2017
San Raffaele University of Rome
2015-2016
Transplanted neurons derived from stem cells have been proposed to improve function in animal models of human disease by various mechanisms such as neuronal replacement. However, whether the grafted receive functional synaptic inputs recipient's brain and integrate into host neural circuitry is unknown. Here we studied cortical induced pluripotent after transplantation stroke-injured rat cerebral cortex. Using rabies virus-based trans-synaptic tracing method immunoelectron microscopy,...
A hallmark of adult hematopoiesis is the continuous replacement blood cells with limited lifespans. While active hematopoietic stem cell (HSC) contribution to multilineage foundation clinical HSC transplantation, recent reports have questioned physiological HSCs normal/steady-state hematopoiesis. Here, we use inducible lineage tracing from genetically marked and reveal robust HSC-derived This commences via defined progenitor cells, but varies substantially in between different lineages. By...
COVID-19 typically manifests as a respiratory illness, but several clinical reports have described gastrointestinal symptoms. This is particularly true in children whom symptoms are frequent and viral shedding outlasts clearance from the system. These observations raise question of whether virus can replicate within stomach. Here we generate gastric organoids fetal, pediatric, adult biopsies vitro models SARS-CoV-2 infection. To facilitate infection, induce reverse polarity organoids. We...
Choroid plexus (CP) supports the entry of monocyte-derived macrophages (MDMs) to central nervous system in animal models traumatic brain injury, spinal cord and Alzheimer's disease. Whether CP is involved recruitment MDMs injured after ischemic stroke unknown.Adult male C57BL/6 mice were subjected focal cortical ischemia by permanent occlusion distal branch right middle cerebral artery. tissues collected analyzed for Vcam1, Madcam1, Cx3cl1, Ccl2, Nt5e, Ifnγ expression at different timepoints...
In multiple sclerosis, the pathological interaction between autoreactive Th cells and mononuclear phagocytes in CNS drives initiation maintenance of chronic neuroinflammation. Here, we found that intrathecal transplantation neural stem/precursor (NPCs) mice with experimental autoimmune encephalomyelitis (EAE) impairs accumulation inflammatory monocyte-derived (MCs) CNS, leading to improved clinical outcome. Secretion IL-23, IL-1, TNF-α, cytokines required for terminal differentiation cells,...
Several neurodegenerative diseases cause loss of cortical neurons, leading to sensory, motor, and cognitive impairments. Studies in different animal models have raised the possibility that transplantation human neuronal progenitors, generated from pluripotent stem cells, might be developed into a novel therapeutic strategy for disorders affecting cerebral cortex. For example, we shown long-term neuroepithelial-like (lt-NES) cell-derived produced induced cells transplanted stroke-injured...
Pancreatic cancer is likely to become one of the leading causes cancer-related death in many countries within next decade. Surgery potentially curative treatment for pancreatic ductal adenocarcinoma (PDAC), although only 10%-20% patients have a resectable disease after diagnosis. Despite recent advances surgery current prognosis ranges from 6% 10% globally. One main issues at pre-clinical level lacking model which simultaneously reflects tumour microenvironment (TME) both structural and...
Induced pluripotent stem cell–derived (iPS-derived) neural precursor cells may represent the ideal autologous cell source for cell-based therapy to promote remyelination and neuroprotection in myelin diseases. So far, therapeutic potential of reprogrammed has been evaluated neonatal demyelinating models. However, repair efficacy safety these not well addressed demyelinated adult CNS, which decreased plasticity scarring. Moreover, it is clear if induced pluripotent–derived have same...
Recent advancements in cell engineering have succeeded manipulating identity with the targeted overexpression of specific fate determining transcription factors a process named transcriptional programming. Neurogenin2 (NGN2) is sufficient to instruct pluripotent stem cells (PSCs) acquire neuronal when delivered an integrating system, which arises some safety concerns for clinical applications. A non-integrating system based on modified messenger RNA (mmRNA) delivery method, represents...
Induced pluripotent stem cells (iPSCs) or their progeny, derived from human somatic cells, can give rise to functional improvements after intracerebral transplantation in animal models of stroke. Previous studies have indicated that reactive gliosis, which is associated with stroke, inhibits neurogenesis both endogenous and grafted neural stem/progenitor (NSPCs) rodent origin. Here we assessed whether astrocytes affect the fate iPSC-derived NSPCs transplanted into stroke-injured brain. Mice...
Abstract Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is a global public health emergency. COVID-19 typically manifests as illness but an increasing number of clinical reports describe gastrointestinal (GI) symptoms. This particularly true in children whom GI symptoms are frequent and viral shedding outlasts clearance from the system. By contrast, fetuses seem to be rarely affected COVID-19, although virus has...
Intracerebral transplantation of human induced pluripotent stem cells (iPSCs) can ameliorate behavioral deficits in animal models stroke. How the ischemic lesion affects survival transplanted cells, their proliferation, migration, differentiation, and function is only partly understood. Here we have assessed influence stroke-induced injury on grafts skin iPSCs-derived long-term neuroepithelial-like using into rostral migratory stream (RMS), adjacent to neurogenic subventricular zone, adult...
Abstract Human cellular reprogramming to induced pluripotency is still an inefficient process, which has hindered studying the role of critical intermediate stages. Here we take advantage high efficiency in microfluidics and temporal multi-omics identify resolve distinct sub-populations their interactions. We perform secretome analysis single-cell transcriptomics show functional extrinsic pathways protein communication between re-shaping a permissive extracellular environment. pinpoint...
Abstract Oligodendrocytes are extensively coupled to astrocytes, a phenomenon ensuring glial homeostasis and maintenance of central nervous system myelin. Molecular disruption this communication occurs in demyelinating diseases such as multiple sclerosis. Less is known about the vulnerability reconstruction panglial network during adult demyelination‐remyelination. Here, we took advantage lysolcithin‐induced demyelination investigate expression dynamics oligodendrocyte specific connexin 47...
Abstract Human neuromuscular organoids (NMOs) derived from induced pluripotent stem cells (hiPSCs) hold a great potential to study (dys)functional human skeletal muscle (SkM) in vitro. The three-dimensional (3D) self-assembly of NMOs leads the generation spheroids, whose 3D organization cannot be controlled. Indeed, proper development, maturation and function innervated SkM require well-defined multiscale tissue-specific extracellular matrix (ECM) context. We hypothesized that structural...
The human developmental processes during the early post-implantation stage instruct specification and organization of lineage progenitors into a body plan. These processes, which include patterning, cell sorting, establishment three germ layers, have been classically studied in non-human model organisms only recently, through micropatterning technology, human-specific context. Micropatterning technology has unveiled mechanisms patterning layer specification; however, sorting their...
Summary Human central nervous system (CNS) development involves complex transitions from pluripotency to regionalised neural tissues. The early phases of this process are inaccessible in humans but can potentially be modelled vitro using brain organoids, including study neurodevelopmental disorders. However, current methods based on post-implantation-like human pluripotent stem cells (hPSCs), which exhibit a hypermethylated state and show epigenetic memory retention. Here we developed 3D...
Abstract Skeletal muscle (SkM) tissue engineering aims to generate in vitro three-dimensional (3D) products that can be implanted patients replace or repair damaged muscles. Having a humanized model able mimic the interaction between innervated recipient and engineered SkMs at functional level would greatly help evaluation of graft potential. Here we developed 3D allowed investigate function, stability, adaptability human NM system response an SkM construct. To achieved this, used...