A5016221605- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Multiple Sclerosis Research Studies
- Pluripotent Stem Cells Research
- Hereditary Neurological Disorders
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Advanced Neuroimaging Techniques and Applications
- Spinal Dysraphism and Malformations
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Neurogenetic and Muscular Disorders Research
- Epigenetics and DNA Methylation
- Neuropeptides and Animal Physiology
- Prion Diseases and Protein Misfolding
- Immune cells in cancer
- Neonatal and fetal brain pathology
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Neuroscience and Neuropharmacology Research
- Advanced MRI Techniques and Applications
- Olfactory and Sensory Function Studies
Institut du Cerveau
2012-2023
Centre National de la Recherche Scientifique
2012-2023
Sorbonne Université
2012-2023
Pitié-Salpêtrière Hospital
1995-2023
Assistance Publique – Hôpitaux de Paris
2007-2023
Inserm
2012-2023
Université Paris Cité
2015-2022
Canadian Nautical Research Society
2019-2020
Allen Institute for Brain Science
2019
Centre de Gestion Scientifique
2011
Abstract The effect of mouse nerve growth factor (NGF) on cultured human fetal sensory neurons was assayed by measuring neurite length, density and rate growth. Addition NGF increased adhesion dissociated collagen coated surfaces. Almost all 9 to 10 week old fetuses are postmitotic, contain neuron‐specific enolase, (an enzyme linked differentiation), require for optimal Sensory ganglia re‐explanted showed maximal length when treated with 1 ng/ml NGF. Neurite reduced considerably in the...
Abstract Identifying a source of cells with the capacity to generate oligodendrocytes in adult CNS would help development strategies promote remyelination. In present study, we examined ability precursor mouse subventricular zone (SVZ) differentiate into remyelinating oligodendrocytes. After lysolecithin‐induced demyelination corpus callosum, progenitors rostral SVZ (SVZa) and migratory pathway (RMS), expressing embryonic polysialylated form neural cell adhesion molecule (PSA‐NCAM),...
The destiny of the mitotically active cells subventricular zone (SVZ) in adult rodents is to migrate olfactory bulb, where they contribute replacement granular and periglomerular neurons. However, these neural progenitors also can be mobilized periventricular white matter triggered differentiate into astrocytes oligodendrocytes response lysolecithin-induced demyelination. To mimic environmental conditions multiple sclerosis, we assessed proliferation, migration, differentiation potential SVZ...
In multiple sclerosis (MS), oligodendrocyte and myelin destruction lead to demyelination with subsequent axonal loss. Experimental in rodents has highlighted the activation of subventricular zone (SVZ) involvement progenitor cells expressing polysialylated form neural cell adhesion molecule (PSA-NCAM) repair process. this article, we studied distribution early PSA-NCAM + progenitors SVZ MS lesions human postmortem brains. Compared controls, showed a 2- 3-fold increase density proliferation,...
Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) are currently lacking, but may be a key technology understand the biology of myelin diseases develop treatments for such disorders. Here, we demonstrate that induction three transcription factors (SOX10, OLIG2, NKX6.2) in iPSC-derived neural progenitor is sufficient rapidly O4+ OL with an efficiency up 70% 28 d global gene-expression profile comparable primary OL. We further...
The subventricular zone (SVZ) contains undifferentiated cells, which proliferate and generate olfactory bulb (OB) interneurons. Throughout life, these cells leave the SVZ migrate along rostral migratory stream (RMS) to OB where they differentiate. In vitro , septum choroid plexus (CP) secrete repulsive factors that could orient migration of precursors. Slit1 Slit2, two known chemorepellents for developing axons, can mimic this effect. We show here Slit receptors Robo2 Robo3/Rig-1 are...
Techniques are now available for culturing well characterized and purified Schwann cells. Therefore, we investigated the role of fibronectin in adhesion, growth, migration cultured rat Double-immunolabeling shows that, primary cultures sciatic nerve, cells (90%) rarely express fibronectin, whereas fibroblasts (10%) exhibit a granular cytoplasmic fibrillar surface-associated fibronectin. Secondary do not Exogenous has small effect on promoting adhesion to substrate does significantly affect...
Abstract We have investigated the expression of highly polysialylated neural cell adhesion molecule in mouse spinal cord during postnatal myelination and adult after chemically induced demyelination. By double immunohistochemistry, using a monoclonal antibody (anti‐Men B) which specifically recognizes polysialic acid (PSA) units on (N‐CAM), an anti‐myelin basic protein, caudorostral gradient PSA‐NCAM was observed at day 1 (P1), inversely related to myelination. At P7, labelling decreased...
Abstract Using a solution‐hybridization assay and specific oligonucleotidic probes, we have studied IGF‐I insulin receptor mRNAs in the rat central nervous system during development. The expression of was maximal at embryonic day 15 20 for receptors, birth receptors. After birth, both transcripts decreased reached minimal levels adult. At time which these were maximally expressed (embryonic 20), regional analysis indicated that widely distributed brain. In contrast, restricted to certain...
Neural stem cells (NSCs) persist in defined brain niches, including the subventricular zone (SVZ), throughout adulthood and generate new neurons destined to support specific neurological functions. Whether diseases such as multiple sclerosis (MS) are associated with changes adult NSCs whether this might contribute development and/or persistence of deficits remains poorly investigated. We examined SVZ function mice which we targeted an MS-like pathology forebrain. In these mice, refer herein...
The synthesis of platelet-derived growth factor-α receptor (PDGF-αR) is commonly attributed to oligodendrocyte progenitors during late embryonic and postnatal development. However, we recently demonstrated that mature neurons could also synthesize PDGF-αR, emphasizing a larger role for this than previously described. In the present study, analyze pattern PDGF-αR expression development mouse CNS, used in situ hybridization immunohistochemistry on brain spinal cord tissue sections. We found...
Experimental studies provided overwhelming proof that transplants of myelin-forming cells achieve efficient remyelination in the CNS. Among cellular candidates, Schwann can be used for autologous transplantation to ensure robust lesions and deliver therapeutic factors In present study, macaque expressing green fluorescent protein (GFP) were infected with human immunodeficiency virus-derived vectors overexpressing brain-derived neurotrophic factor (BDNF) or Neurotrophin 3 (NT-3), two...
The limited availability of enriched populations oligodendroglial progenitors has impeded the exploration complex spatio-temporal mechanisms which dictate chemical "language" their biology. We have developed a technique to prepare homotypic aggregates oligodendrocyte called "oligospheres." These were obtained using various approaches (sieving, Percoll gradient separation and differential adhesion) purify from newborn rat brain. Culturing cells in mixture N1 defined medium conditioned B104...
Neonatal rat Schwann cells isolated in culture proliferate slowly and do not form a basement membrane although they express laminin continuously. We demonstrate here that other components, including entactin heparan sulfate proteoglycan. Treatment with ascorbate, to lesser extent cyclic adenosine 3'',5''-monophosphate, modulates the synthesis of extracellular matrix components by cultured cells. After this treatment, fibronectin collagen type IV are detected on cell surface, which form,...
The vitamin D receptor (VDR) is a nuclear that mediates the effect of active metabolite D3, 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). To investigate potential role this hormone in peripheral nervous system, we have studied VDR expression Schwann cells. mRNA was detected by Northern blot analysis rat primary cultures cells, and its levels were strongly increased presence 1,25-(OH)2D3. Using mouse cell line, MSC80, showed concentrations as low 10(-10) M stimulated gene amounts activated VDR,...