A5085485880- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Modular Robots and Swarm Intelligence
- Additive Manufacturing and 3D Printing Technologies
- Kruppel-like factors research
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
University of Padua
2018-2023
Babraham Institute
2022-2023
Conventional human pluripotent stem cells (hPSCs) are widely used to study early embryonic development, generate somatic cells, and model diseases, with differentiation potential aligned a post-implantation epiblast identity. In the past decade, naive hPSCs, representing pre-implantation stage, have been derived. Naive hPSCs efficiently differentiate towards extraembryonic lineages such as trophectoderm, primitive endoderm, mesoderm, also self-organize into blastocyst-like structures called...
Abstract Human pluripotent stem cells (hPSCs) have the capacity to give rise all differentiated of adult. TGF-beta is used routinely for expansion conventional hPSCs as flat epithelial colonies expressing transcription factors POU5F1/OCT4, NANOG, SOX2. Here we report a global analysis transcriptional programme controlled by followed an unbiased gain-of-function screening in multiple hPSC lines identify mediating activity. We quartet regulators promoting self-renewal including ZNF398,...
In human embryos, naive pluripotent cells of the inner cell mass (ICM) generate epiblast, primitive endoderm and trophectoderm (TE) lineages, whence trophoblast derive. vitro, stem (PSCs) retain this potential efficiently (TSCs), while conventional PSCs form TSCs at low efficiency. Transient histone deacetylase MEK inhibition combined with LIF stimulation is used to chemically reset PSCs. Here, we report that chemical resetting induces expression both TSC markers placental imprinted genes. A...
The establishment of in vitro naïve human pluripotent stem cell cultures opened new perspectives for the study early events development. role several transcription factors and signaling pathways during maintenance pluripotency has been characterized. However, little is known about exerted by extracellular matrix its three-dimensional organization. Here, using an unbiased integrated approach combining transcriptional, proteomic secretome analyses, we found that naïve, but not primed, hiPSC...
Abstract Pluripotency is the ability to give rise all cell types of body and first observed in a mass disorganised cells embryo. Upon implantation, pluripotent form columnar epithelium undergo lumenogenesis. At gastrulation, portion epiblast will epithelial mesenchymal transition (EMT), forming primitive streak (PS). It still remains unclear what molecular mechanism supports identity before gastrulation. Here we developed an optimised, chemically defined 3D model human formation which...
ABSTRACT Pluripotency is the capacity to give rise all differentiated cells of body and germ line governed by a self-reinforcing network transcription factors. The forced expression only some these factors enables reprogramming somatic pluripotency. In murine cells, several kruppel-like (KLFs) have been identified as stabilisers inducers Human are routinely reprogrammed KLF4 in combination with OCT4, SOX2 cMYC (OSKM). An extensive transcriptome analysis revealed, however, that barely...
Abstract In human embryos, naive pluripotent cells of the inner cell mass generate epiblast, primitive endoderm and Trophectoderm (TE) lineage, whence trophoblast derive. vitro , stem (PSCs) retain this potential can (TSCs), while conventional PSCs form amnion-like lack competence to TSCs. Transient histone deacetylase MEK inhibitions with LIF stimulation be used chemically reset PSCs. Here we report that chemical resetting induced expression both TSC markers placental imprinted genes. A...