A5038395336- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Hair Growth and Disorders
- Hemophilia Treatment and Research
- Renal and related cancers
- Skin and Cellular Biology Research
- Wound Healing and Treatments
- Urban Green Space and Health
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Immune responses and vaccinations
- Genetic Syndromes and Imprinting
- Circular RNAs in diseases
- Immune cells in cancer
- Muscle Physiology and Disorders
- Scientific Computing and Data Management
- Zoonotic diseases and public health
- Melanoma and MAPK Pathways
- TGF-β signaling in diseases
- Autoimmune Bullous Skin Diseases
University of Turin
2018-2024
Italian institute for Genomic Medicine
2018-2024
Regione del Veneto
2023
Ferdowsi University of Mashhad
2021
Sharif University of Technology
2021
Iran University of Medical Sciences
2021
Tarbiat Modares University
2021
Accademia Albertina delle Belle Arti
2018-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Abstract Epithelial cells that participated in wound repair elicit a more efficient response to future injuries, which is believed be locally restricted. Here we show cell adaptation resulting from localized tissue damage has wide spatial impact at scale not previously appreciated. We demonstrate specific stem population, distant the original injury, originates long-lasting memory progenitors residing their own niche. Notably, these distal have taken part first healing but become...
Abstract The correct establishment of DNA methylation patterns during mouse early development is essential for cell fate specification. However, the molecular targets as well mechanisms that determine specificity de novo machinery differentiation are not completely elucidated. Here we show DNMT3B-dependent key developmental regulatory regions at epiblast-like cells (EpiLCs) provides an epigenetic priming ensures flawless commitment later stages. Using in vitro stem and loss function...
The histone acetyltransferase p300 (also known as KAT3B) is a general transcriptional coactivator that introduces the H3K27ac mark on enhancers triggering their activation and gene transcription. Genome-wide screenings demonstrated large fraction of long non-coding RNAs (lncRNAs) plays role in cellular processes organ development although underlying molecular mechanisms remain largely unclear (1,2). We found 122 lncRNAs interacts directly with p300. In depth analysis one these, lncSmad7,...
miR-29b2 and miR-29c play a suppressive role in breast cancer progression.
Background Next generation sequencing methods are widely adopted for a large amount of scientific purposes, from pure research to health-related studies. The decreasing costs per analysis led big amounts generated data and the subsequent improvement software respective analyses. As consequence, many approaches have been developed chain different in order obtain reliable reproducible workflows. However, range applications NGS entails challenge manage workflows without losing reliability....
Background. Urban planning is a key tool to promote health in cities. The COVID-19 emergency accelerated several social, environmental, and digital challenges, stressing the importance of some issues regarding housing, urban mobility, green areas, service networks, policies, actions. These were subject an intensive residential course (the 57th) held Erice, Sicily, June 2021 “International School Epidemiology Preventive Medicine G. D’Alessandro”, main findings are described here. Methods....
Heterogeneity is a fundamental feature of complex phenotypes. So far, genomic screenings have profiled thousands samples providing insights into the transcriptome cell. However, disentangling heterogeneity these transcriptomic Big Data to identify defective biological processes remains challenging. Here we present GSECA, method exploiting bimodal behavior RNA-sequencing gene expression profiles altered sets in heterogeneous patient cohorts. Using simulated and experimental data sets, show...
Smad7 has been identified as a negative regulator of the transforming growth factor TGF-β pathway by direct interaction with type I receptor (TβR-I). Although also shown to play unrelated functions in cytoplasm and nucleus, comprehensive analysis its nuclear function not yet performed. Here, we show that ESCs is mainly acts general transcription regulating several genes pathway. Loss results downregulation key stemness master regulators, including Pou5f1 Zfp42, upregulation developmental...
Patients with breast cancer show altered expression of genes within the pectoralis major skeletal muscle cells breast. Through analyses The Cancer Genome Atlas (TCGA)-breast (BRCA), we identified three previously uncharacterized putative novel tumor suppressor expressed in normal cells, whose was downregulated tumors. We found that NEDD4 binding protein 2-like 1 ( N4BP2L1 ), pleckstrin homology domain-containing family A member 4 PLEKHA4 and brain-enriched guanylate kinase-associated BEGAIN...
Abstract It is believed epithelial cells that have participated in a wound repair elicit more efficient but locally restricted response to future injuries. However here we show the cell adaptation resulting from localised tissue damage has wide spatial impact at scale not previously noticed. We demonstrate away injured site, after first injury specific stem population gives rise long term wound-memory progenitors residing their own niche of origin. Notably these taken part healing become...
<title>Abstract</title> MIR29B2CHG/C1orf132 is the host gene for generating miR-29b2 and miR-29c. Here, we employed bioinformatics experimental approaches to decipher expression of C1orf132 in breast cancer cells tissues. Our data demonstrated a significant downregulation triple-negative cancer. We also predicted putative promoter longer transcripts C1orf132. The functionality distal was confirmed by transfecting MCF7 with promoter-GFP construct. Knocking-out means CRISPR/Cas9 approach...
Abstract Haemophilia A (HA) is a rare bleeding disorder caused by factor 8 (F8) mutations. Clinical manifestations are spontaneous bleedings that primarily consist of hemarthrosis and intracranial haemorrhages. To date, the impairment vessel stability in HA patients correlation between FVIII endothelial functionality poorly understood. Here we show plays role cell functionality. Blood Outgrowth cells (BOECs) knockout generated CRISPR/Cas9, BOECs iPSCs-derived ECs showed alteration...
Abstract Pluripotency is the ability to give rise all cell types of body and first observed in a mass disorganised cells embryo. Upon implantation, pluripotent form columnar epithelium undergo lumenogenesis. At gastrulation, portion epiblast will epithelial mesenchymal transition (EMT), forming primitive streak (PS). It still remains unclear what molecular mechanism supports identity before gastrulation. Here we developed an optimised, chemically defined 3D model human formation which...
Abstract Prostate cancer (PCa) is genomically driven by dysregulation of transcriptional networks involving the factors (TFs) FOXA1, ERG, AR, and HOXB13. However, role these specific TFs in regulation alternative pre-mRNA splicing (AS), which a proven therapeutic vulnerability for cancers TF MYC, not described. Using transcriptomic datasets from PCa patients, we tested an association between expression HOXB13 , MYC genes involved AS - termed splicing-related proteins (SRPs), have pleiotropic...
Abstract Injury in adult tissue generally reactivates developmental programs to foster regeneration, but it is not known whether this paradigm applies growing tissue. Here, by employing blisters, we show that epidermal wounds heal at the expense of skin development. The regenerated epidermis suppresses expression morphogenesis genes accompanied delayed hair follicle (HF) growth. Lineage tracing experiments, cell proliferation dynamics, and mathematical modeling reveal progeny HF junctional...
1. Abstract Long non-coding RNAs (lncRNAs) regulate gene expression through different molecular mechanisms, including DNA binding. We curated the first database of RNA Binding Sites (RNABSdb) by harmonising publicly available raw-data RNA-DNA binding experiments. This resource is crucial to enable systematic studies on transcriptional regulation driven lncRNAs. Focusing high quality experiments, we find that number sites for each lncRNAs varies from hundreds tens thousands. Despite being...