A5075168526- Colorectal Cancer Treatments and Studies
- PI3K/AKT/mTOR signaling in cancer
- Melanoma and MAPK Pathways
- CRISPR and Genetic Engineering
- Genetic factors in colorectal cancer
- Gastric Cancer Management and Outcomes
- Lung Cancer Treatments and Mutations
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Cellular transport and secretion
- Pancreatic and Hepatic Oncology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Advanced Breast Cancer Therapies
- Metabolism, Diabetes, and Cancer
- Protein Degradation and Inhibitors
- Cancer Cells and Metastasis
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Chronic Lymphocytic Leukemia Research
- Epigenetics and DNA Methylation
- Neuroendocrine Tumor Research Advances
University of Turin
2015-2024
Karolinska Institutet
2024
Università Cattolica del Sacro Cuore
2014
IFOM
2013
Candiolo Cancer Institute
2008-2013
Ospedale San Giovanni Bellinzona
2008
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2008
Istituto Cantonale di Patologia
2008
Istituto Superiore di Sanità
2007
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2007
PURPOSE Cetuximab or panitumumab are effective in 10% to 20% unselected metastatic colorectal cancer (CRC) patients. KRAS mutations account for approximately 30% 40% patients who not responsive. The serine-threonine kinase BRAF is the principal effector of KRAS. We hypothesized that, wild-type patients, could have a predictive/prognostic value. PATIENTS AND METHODS retrospectively analyzed objective tumor responses, time progression, overall survival (OS), and mutational status 113 tumors...
The monoclonal antibodies (moAb) panitumumab and cetuximab target the epidermal growth factor receptor (EGFR) have proven valuable for treatment of metastatic colorectal cancer (mCRC). EGFR-mediated signaling involves two main intracellular cascades: on one side KRAS activates BRAF, which in turn triggers mitogen-activated protein kinases. On other, membrane localization lipid kinase PIK3CA counteracts PTEN promotes AKT1 phosphorylation, thereby activating a parallel axis. Constitutive...
Background: Anthracyclines, such as doxorubicin (DOX), are potent anticancer agents for the treatment of solid tumors and hematologic malignancies. However, their clinical use is hampered by cardiotoxicity. This study sought to investigate role phosphoinositide 3-kinase γ (PI3Kγ) in DOX-induced cardiotoxicity potential cardioprotective effects PI3Kγ inhibition. Methods: Mice expressing a kinase-inactive or receiving PI3Kγ-selective inhibitors were subjected chronic DOX treatment. Cardiac...
Background KRAS mutations occur in 35–45% of metastatic colorectal cancers (mCRC) and preclude responsiveness to EGFR-targeted therapy with cetuximab or panitumumab. However, less than 20% patients displaying wild-type tumors achieve objective response. Alterations other effectors downstream the EGFR, such as BRAF, deregulation PIK3CA/PTEN pathway have independently been found give rise resistance. We present a comprehensive analysis KRAS, PIK3CA mutations, PTEN expression mCRC treated...
Multiple phosphatidylinositol (PtdIns) 3-kinases (PI3Ks) can produce PtdIns3P to control endocytic trafficking, but whether enzyme specialization occurs in defined subcellular locations is unclear. Here, we report that PI3K-C2α enriched the pericentriolar recycling compartment (PRE) at base of primary cilium, where it regulates production a specific pool PtdIns3P. Loss PI3K-C2α-derived leads mislocalization PRE markers such as TfR and Rab11, reduces Rab11 activation, blocks accumulation Rab8...
Podocyte injury leading to albuminuria is a characteristic feature of diabetic nephropathy (DN). Hyperglycemia and advanced glycation end products (AGEs) are major determinants DN. However, the underlying mechanisms podocyte remain poorly understood. The cytosolic protein TNFAIP2/M-Sec required for tunneling nanotubes (TNTs) formation, which membrane channels that transiently connect cells, allowing organelle transfer. Podocytes express TNFAIP2 form TNTs, but potential relevance TNFAIP2-TNT...
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive form of pancreatic cancer, known for its challenging diagnosis and limited treatment options. The focus on metabolic reprogramming as key factor in tumor initiation, progression, therapy resistance has gained prominence. In this review we the impact changes interplay among stromal, immune, cells, glutamine branched-chain amino acids (BCAAs) emerge pivotal players modulating immune cell functions growth. We also discuss ongoing...
Mutations in oncogenes and tumor suppressor genes are responsible for tumorigenesis represent favored therapeutic targets oncology. We exploited homologous recombination to knock-in individual cancer mutations the genome of nontransformed human cells. Sequential introduction multiple was also achieved, demonstrating potential this strategy construct progression models. Knock-in cells displayed allele-specific activation signaling pathways mutation-specific phenotypes different from those...
The phosphatidylinositol 3-kinase (PI3K) signaling pathway regulates several cellular processes and it's one of the most frequently deregulated in human tumors. Given its prominent role cancer, there is great interest development inhibitors able to target members PI3K clinical trials. These drug candidates include inhibitors, both pan- isoform-specific AKT, mTOR, dual PI3K/mTOR inhibitors. As novel compounds progress into trials, becoming urgent identify select patient population that likely...
Cytokinetic membrane abscission is a spatially and temporally regulated process that requires ESCRT (endosomal sorting complexes required for transport)–dependent control of remodeling at the midbody, subcellular organelle defines cleavage site. Alteration function can lead to cataract, but underlying mechanism its relation cytokinesis are unclear. We found lens-specific cytokinetic PI3K-C2α (phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2α), lipid product PI(3,4)P
Background: Avoidant restrictive food intake disorder (ARFID) is a feeding and eating disorder, characterized by limited variety and/or quantity of impacting physical health psychosocial functioning. Children with ARFID often present range psychiatric somatic symptoms, therefore consult various pediatric subspecialties; large-scale studies mapping comorbidities are however lacking. To characterize care needs people ARFID, we systematically investigated ARFID-related mental conditions in 616...
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread global pandemic we are facing. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) currently used for clinical diagnosis SARS-CoV-2 infection using nasopharyngeal swabs, tracheal aspirates, or bronchoalveolar lavage (BAL) samples. Despite high sensitivity qRT-PCR method, false negative outcomes might occur, especially in patients with a...
Carcinomas are comprised of transformed epithelial cells that supported in their growth by a dedicated neovasculature. How the genetic milieu compartment influences tumor angiogenesis is largely unexplored. Drugs targeted to mutant cancer genes may act not only on but also, directly or indirectly, surrounding stroma. We investigated role BRAF V600E oncogene tumor/vessel crosstalk and analyzed effect inhibitor PLX4720 angiogenesis. Knock-in allele into genome human triggered angiogenic...