A5032170083- Cancer Cells and Metastasis
- Hippo pathway signaling and YAP/TAZ
- TGF-β signaling in diseases
- Cancer-related Molecular Pathways
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Connexins and lens biology
- Pancreatic and Hepatic Oncology Research
- CRISPR and Genetic Engineering
- Axon Guidance and Neuronal Signaling
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Lipid metabolism and biosynthesis
- FOXO transcription factor regulation
- Epigenetics and DNA Methylation
- Congenital limb and hand anomalies
University of Padua
2011-2024
The Francis Crick Institute
2017-2021
Abstract Three-dimensional hydrogel-based organ-like cultures can be applied to study development, regeneration, and disease in vitro. However, the control of engineered hydrogel composition, mechanical properties geometrical constraints tends restricted initial time fabrication. Modulation characteristics over according culture evolution is often not possible. Here, we overcome these limitations by developing a hydrogel-in-hydrogel live bioprinting approach that enables dynamic fabrication...
During development and tissue homeostasis, cells must integrate different signals. We investigated how cell behavior is controlled by the combined activity of transforming growth factor-beta (TGF-beta) receptor tyrosine kinase (RTK) signaling, whose integration mechanism unknown. find that RTK/Ras/MAPK (mitogen-activated protein kinase) induces p53 N-terminal phosphorylation, enabling interaction with TGF-beta-activated Smads. This confines mesoderm specification in Xenopus embryos promotes...
Abstract Altered nuclear shape is a defining feature of cancer cells. The mechanisms underlying dysmorphia in remain poorly understood. Here we identify PPP1R12A and PPP1CB, two subunits the myosin phosphatase complex that antagonizes actomyosin contractility, as proteins safeguarding integrity. Loss or PPP1CB causes fragmentation, envelope rupture, compartment breakdown genome instability. Pharmacological genetic inhibition contractility restores architecture integrity cells lacking PPP1CB....
Abstract Human pluripotent stem cells (hPSCs) have the capacity to give rise all differentiated of adult. TGF-beta is used routinely for expansion conventional hPSCs as flat epithelial colonies expressing transcription factors POU5F1/OCT4, NANOG, SOX2. Here we report a global analysis transcriptional programme controlled by followed an unbiased gain-of-function screening in multiple hPSC lines identify mediating activity. We quartet regulators promoting self-renewal including ZNF398,...
The shuttling of transcription factors and transcriptional regulators into out the nucleus is central to regulation many biological processes. Here we describe a new method for studying rates nuclear entry exit regulators. A photo-responsive LOV (light-oxygen-voltage) domain from Avena sativa used sequester fluorescently labelled YAP1 TAZ (also known as WWTR1) on surface mitochondria reversibly release them upon blue light illumination. After dissociation, fluorescent signals mitochondria,...
Late relapse of disseminated cancer cells is a common feature breast and prostate tumors. Several intrinsic extrinsic factors have been shown to affect quiescence reawakening dormant (DDCCs); however, the signals processes sustaining survival DDCCs in foreign environment are still poorly understood. We recently that crosstalk with lung epithelial promotes estrogen receptor-positive (ER+) By using organotypic system vivo dissemination assays, here we show TFEB-lysosomal axis activated it...
The high mutation rate of SARS-CoV-2 leads to the emergence multiple variants, some which are resistant vaccines and drugs targeting viral elements. Targeting host dependency factors, e.g. cellular proteins required for replication, would help prevent development resistance. However, it remains unclear whether different variants induce conserved responses exploit same core factors. To this end, we compared three concern found that transcriptional response was conserved, differing only in...
The Spemann organizer stands out from other signaling centers of the embryo because its broad patterning effects. It defines development along anteroposterior and dorsoventral axes vertebrate body, mainly by secreting antagonists growth factors. Qualitative models proposed more than a decade ago explain organizer’s region-specific inductions (i.e., head trunk) as result different combinations antagonists. For example, induction is mediated extracellular inhibition Wnt, BMP, Nodal ligands....
(1) Background: metastatic relapse following a prolonged period of disease-free survival is common cause mortality for many cancer patients. Disseminated dormant cells (DDCCs) lie below the radar before waking up years, or even decades, after removal primary tumor. This implies that they are able to survive in latent state foreign environment an extended time supported by intrinsic and extrinsic factors still be elucidated. (2) Methods: we employed coculture DDCCs with lung epithelial...
Abstract The shuttling of transcription factors and transcriptional regulators in out the nucleus is central to regulation many biological processes. Here we describe a new method for studying rates nuclear entry exit regulators. A photo-responsive AsLOV ( Avena sativa Light Oxygen Voltage) domain used sequester fluorescently-labelled YAP1 TAZ/WWTR1 on surface mitochondria reversibly release them upon blue light illumination. After dissociation, fluorescent signals from mitochondria,...