A5064750899- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- Renal and related cancers
- CAR-T cell therapy research
- TGF-β signaling in diseases
- Developmental Biology and Gene Regulation
- Modular Robots and Swarm Intelligence
- Ubiquitin and proteasome pathways
- Nanowire Synthesis and Applications
- Hippo pathway signaling and YAP/TAZ
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroscience and Neural Engineering
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Organ Donation and Transplantation
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Genetic and Kidney Cyst Diseases
- Skin and Cellular Biology Research
University of Southern California
2017-2024
Broad Center
2017-2024
Southern California University for Professional Studies
2021-2024
Eli and Edythe Broad Foundation
2023
University of California, San Francisco
2013-2019
Viterbo University
2019
Howard Hughes Medical Institute
2013-2018
University of Padua
2007-2011
A common theme in the self-organization of multicellular tissues is use cell-cell signaling networks to induce morphological changes. We used modular synNotch juxtacrine platform engineer artificial genetic programs which specific contacts induced changes cadherin cell adhesion. Despite their simplicity, these minimal intercellular were sufficient yield assemblies with hallmarks natural developmental systems: robust into multidomain structures, well-choreographed sequential assembly, type...
Synthetic Notch (synNotch) receptors are genetically encoded, modular synthetic that enable mammalian cells to detect environmental signals and respond by activating user-prescribed transcriptional programs. Although some materials have been modified present synNotch ligands with coarse spatial control, applications in tissue engineering generally require extracellular matrix (ECM)-derived scaffolds and/or finer positioning of multiple ligands. Thus, we develop here a suite activate for...
The definition of embryonic potency and induction specific cell fates are intimately linked to the tight control over TGFbeta signaling. Although extracellular regulation ligand availability has received considerable attention in recent years, surprisingly little is known about intracellular factors that negatively Smad activity mammalian tissues. By means genetic ablation, we show Smad4 inhibitor ectodermin (Ecto, also as Trim33 or Tif1gamma) required limit Nodal responsiveness vivo. New...
Abstract Synthetic Notch (synNotch) receptors are modular synthetic components that genetically engineered into mammalian cells to detect signals presented by neighboring and respond activating prescribed transcriptional programs. To date, synNotch has been used program therapeutic pattern morphogenesis in multicellular systems. However, cell-presented ligands have limited versatility for applications require spatial precision, such as tissue engineering. address this, we developed a suite...
Abstract Tissue engineering approaches, with the goals of replacing or recovering damaged diseased tissues, reconstituting tissues in vitro for disease modeling and drug development, have potential to make significant contributions medicine. Advances stem cell biology, biomaterial synthesis characterization, microscale technologies made engineered a reality. However, classic tools used build lab do not allow complete control behaviors. More recently, synthetic biology principles developed...
Synthetic development is a nascent field of research that uses the tools synthetic biology to design genetic programs directing cellular patterning and morphogenesis in higher eukaryotic cells, such as mammalian cells. One specific example was based on cell-cell contact-dependent signaling using Notch pathways shown drive formation multilayered spheroids by modulating adhesion
Current human pluripotent stem cell-derived kidney organoids contain nephron-like structures that lack organotypic patterning. It is thought during development, nephrons form their proximal-distal axial polarity in response to collecting duct-derived signals are absent organoids. To delineate how nephron polarities establish, we profiled development by spatial transcriptomic approaches. Our analyses describe a new the and demonstrate develops adjacent transcriptional boundary duct where...
Many metazoan organs are comprised of branching trees epithelial tubes; how patterning occurs in these is a fundamental problem development. Commonly, branch tips fill the volume organ approximately uniformly, e.g., mammalian lung, airway dispersed roughly uniformly throughout lung. In contrast, developing metanephric kidney, ureteric bud tree located close to outer surface kidney rather than filling kidney. Here, we describe simple alteration rules that accounts for difference between...
Abstract The aim of the present study was to evaluate regional differences in microvessel density (MVD) human medullary tegmentum adults and newborns/infants. Transverse serial sections formalin‐fixed, paraffin‐embedded brainstems, taken from 16 adult eight newborn/infant subjects, were stained with anti‐von Willebrand factor (vWF) polyclonal antibodies. boundaries area postrema (AP), dorsal motor vagal nucleus (DMVN), solitary tract (STN), (ST) hypoglossal (XII) defined, all vessels counted...