A5058937426- Microtubule and mitosis dynamics
- Micro and Nano Robotics
- Plant Molecular Biology Research
- Remote Sensing and LiDAR Applications
- Cell Image Analysis Techniques
- Plant Reproductive Biology
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Plant nutrient uptake and metabolism
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Kruppel-like factors research
- Congenital gastrointestinal and neural anomalies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood disorders and treatments
- Cellular Mechanics and Interactions
- Acute Myeloid Leukemia Research
- Force Microscopy Techniques and Applications
- Slime Mold and Myxomycetes Research
- Gastrointestinal motility and disorders
- Mechanical and Optical Resonators
- Biofield Effects and Biophysics
- Magnetic Bearings and Levitation Dynamics
- Electric Motor Design and Analysis
- Viral gastroenteritis research and epidemiology
The Francis Crick Institute
2017-2024
Imperial College London
2017-2024
Cancer Research UK
2015
Wake Forest University
2009-2011
Somatic mutations commonly occur in hematopoietic stem cells (HSCs). Some mutant clones outgrow through clonal hematopoiesis (CH) and produce mutated immune progenies shaping host immunity. Individuals with CH are asymptomatic but have an increased risk of developing leukemia, cardiovascular pulmonary inflammatory diseases, severe infections. Using genetic engineering human HSCs (hHSCs) transplantation immunodeficient mice, we describe how a gene CH, TET2, affects neutrophil development...
The shuttling of transcription factors and transcriptional regulators into out the nucleus is central to regulation many biological processes. Here we describe a new method for studying rates nuclear entry exit regulators. A photo-responsive LOV (light-oxygen-voltage) domain from Avena sativa used sequester fluorescently labelled YAP1 TAZ (also known as WWTR1) on surface mitochondria reversibly release them upon blue light illumination. After dissociation, fluorescent signals mitochondria,...
Current knowledge of the transcriptional regulation human pluripotency is incomplete, with lack interspecies conservation observed. Single-cell transcriptomics analysis embryos previously enabled us to identify transcription factors, including zinc-finger protein KLF17, that are enriched in epiblast and naïve embryonic stem cells (hESCs). Here, we show KLF17 expressed coincident known pluripotency-associated factors NANOG SOX2 across blastocyst development. We investigate function using...
Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed and shown to produce piconewton forces. However, some fungal are bidirectional. The force production a bidirectional has not yet been measured. Therefore, it remains unknown whether mechanism unconventional minus-end-directed motility differs fundamentally from that stepping. Using spectroscopy, we have measured here forces...
The molecular motor gliding assay, in which a microtubule or other filament moves across surface coated with motors, has provided much insight into how motors work. kinesin-microtubule system is also strong candidate for the job of nanoparticle transporter nanotechnology devices. In most cases, several transport each filament. Each serves both to bind stationary and propel along surface. By applying uniform transverse force 4-19 pN superparamagnetic bead attached trailing end microtubule, we...
Here we report construction of a simple electromagnet with novel polepieces which apply spatially uniform force to superparamagnetic beads in an optical microscope. The wedge-shaped gap was designed keep ∂Bx/∂y constant and B large enough saturate the bead. We achieved fields 300–600 mT gradients 67 T/m over sample space 0.5×4 mm2 focal plane microscope 0.05 mm along optic axis. Within this maximum on 2.8 μm diameter Dynabead 12 pN spatial variation approximately 10%. Use magnet biophysical...
Abstract In this work, we present a quantitative comparison of the cell division dynamics between populations intact and regenerating root tips in plant model system Arabidopsis thaliana. To achieve required temporal resolution to sustain it for duration regeneration process, adopted live imaging based on light-sheet fluorescence microscopy, previously developed laboratory. We offer straightforward analysis spatial patterns events showing statistically significant difference frequency...
Abstract The shuttling of transcription factors and transcriptional regulators in out the nucleus is central to regulation many biological processes. Here we describe a new method for studying rates nuclear entry exit regulators. A photo-responsive AsLOV ( Avena sativa Light Oxygen Voltage) domain used sequester fluorescently-labelled YAP1 TAZ/WWTR1 on surface mitochondria reversibly release them upon blue light illumination. After dissociation, fluorescent signals from mitochondria,...
Abstract Background Particle-tracking in 3D is an indispensable computational tool to extract critical information on dynamical processes from raw time-lapse imaging. This particularly true with vivo fluorescence imaging cell and developmental biology, where complex dynamics are observed at high temporal resolution. Common tracking algorithms used data microscopy typically assume a continuous signal background, recognisable keypoints independently moving objects of interest permanently...
ABSTRACT In this work, we present a quantitative comparison of the cell division dynamics between populations intact and regenerating root tips in plant model system Arabidopsis thaliana . To achieve required temporal resolution to sustain it for duration regeneration process, adopted live imaging based on light-sheet fluorescence microscopy, previously developed laboratory. We offer straightforward analysis spatial patterns events showing statistically significant difference frequency...
Abstract Background Particle-tracking in 3D is an indispensable computational tool to extract critical information on dynamical processes from raw time-lapse imaging. This particularly true with vivo fluorescence imaging cell and developmental biology, where complex dynamics are observed at high temporal resolution. Common tracking algorithms used data microscopy typically assume a continuous signal background, recognisable keypoints independently moving objects of interest permanently...