A5086233717- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Head and Neck Cancer Studies
- Chronic Lymphocytic Leukemia Research
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Lymphoma Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Hematopoietic Stem Cell Transplantation
- Glaucoma and retinal disorders
- Cancer Cells and Metastasis
- Retinoids in leukemia and cellular processes
- Colorectal and Anal Carcinomas
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer Diagnosis and Treatment
- Global Public Health Policies and Epidemiology
- Immune Cell Function and Interaction
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Intraocular Surgery and Lenses
- Retinal and Macular Surgery
- Air Quality and Health Impacts
- CAR-T cell therapy research
University College London Hospitals NHS Foundation Trust
2016-2025
University College London
2016-2025
King George's Medical University
2010-2024
University College Hospital
2019-2024
Eternal Hospital
2023-2024
Uttarakhand Technical University
2024
CRUK Lung Cancer Centre of Excellence
2014-2023
Cancer Institute (WIA)
2021-2023
Barnsley Hospital NHS Foundation Trust
2023
University of Sheffield
2023
<h2>Summary</h2><h3>Background</h3> Previous attempts to characterise the burden of chronic respiratory diseases have focused only on specific disease conditions, such as obstructive pulmonary (COPD) or asthma. In this study, we aimed globally, providing a comprehensive and up-to-date analysis geographical time trends from 1990 2017. <h3>Methods</h3> Using data Global Burden Diseases, Injuries, Risk Factors Study (GBD) 2017, estimated prevalence, morbidity, mortality attributable through an...
Understanding cancer pathogenesis requires knowledge of not only the specific contributory genetic mutations but also cellular framework in which they arise and function. Here we explore clonal evolution a form childhood precursor–B cell acute lymphoblastic leukemia that is characterized by chromosomal translocation generating TEL-AML1 fusion gene. We identify compartment leukemic children can propagate when transplanted mice. By studying monochorionic twin pair, one preleukemic with frank...
Long COVID is characterized by the emergence of multiple debilitating symptoms following SARS-CoV-2 infection. Its etiology unclear and it often follows a mild acute illness. Anecdotal reports gradual clinical responses to histamine receptor antagonists (HRAs) suggest histamine-dependent mechanism that distinct from anaphylaxis, possibly mediated T cells, which are also regulated histamine. cell perturbations have been previously reported in post-viral syndromes, but landscape patients who...
Populations of genetically identical eukaryotic cells show significant cell-to-cell variability in gene expression. However, we lack a good understanding the origins this variation. We have found marked average cellular rates transcription. also amount mitochondrial mass. undertook fusion studies that suggested transcription rate depends on small diffusible factors. Following this, vitro showed has sensitive dependence [ATP] but not concentration other nucleotide triphosphates (NTPs)....
Somatic mutations commonly occur in hematopoietic stem cells (HSCs). Some mutant clones outgrow through clonal hematopoiesis (CH) and produce mutated immune progenies shaping host immunity. Individuals with CH are asymptomatic but have an increased risk of developing leukemia, cardiovascular pulmonary inflammatory diseases, severe infections. Using genetic engineering human HSCs (hHSCs) transplantation immunodeficient mice, we describe how a gene CH, TET2, affects neutrophil development...
Clinically successful hematopoietic cell transplantation is dependent on stem and progenitor cells. Here we identify the matricellular protein Nephroblastoma Overexpressed (Nov, CCN3) as being essential for their functional integrity. Nov expression restricted to primitive (CD34) compartments of umbilical vein cord blood, its knockdown in these cells by lentivirus-mediated RNA interference abrogates function vitro vivo. Conversely, forced addition recombinant both enhance and/or activity....
Abstract Nasopharyngeal cancer (NPC), endemic in Southeast Asia, lacks effective diagnostic and therapeutic strategies. Even high-income countries the 5-year survival rate for stage IV NPC is less than 40%. Here we report high somatostatin receptor 2 (SSTR2) expression multiple clinical cohorts comprising 402 primary, locally recurrent metastatic NPCs. We show that SSTR2 induced by Epstein–Barr virus (EBV) latent membrane protein 1 (LMP1) via NF-κB pathway. Using cell-based preclinical...
AML1/RUNX1 is the most frequently mutated gene in leukaemia and central to normal biology of hematopoietic stem progenitor cells. However, role different AML1 isoforms within these primitive compartments unclear. Here we investigate whether altering relative expression impacts balance between cell self-renewal differentiation vitro vivo.The human AML1a isoform encodes a truncated molecule with DNA-binding but no transactivation capacity. We used retrovirus-based approach transduce into...
Abstract Background The trial hypothesis was that, in a resource-constrained situation, short-course radiotherapy would improve treatment compliance compared with conventional chemoradiotherapy for locally advanced rectal cancer, without compromising oncological outcomes. Methods In this open-label RCT, patients cT3, cT4 or node-positive non-metastatic cancer were allocated randomly to 5 × Gy and two cycles of XELOX (arm A) concurrent capecitabine B), followed by total mesorectal excision...
Dear Editor, T/myeloid mixed phenotype acute leukaemia (T/M-MPAL) is a rare subtype, probably accounting for <1% of all cases [1]. It characterised by immunophenotypic features both myeloid and T-lymphoid lineages. MPAL distinct from T-cell lymphocytic (T-ALL) (AML) but shares significant molecular genomic similarity to early precursor-like ALL (ETP-ALL). has poorer prognosis than AML, T-ALL ETP-ALL. Therefore, it essential make the correct classification. The study aimed evaluate diagnosis...
The molecular mechanisms governing self-renewal, differentiation, and lineage specification remain unknown. Transcriptional profiling is likely to provide insight into these processes but, as yet, has been confined "static" profiles of stem progenitors cells. We now a comprehensive, statistically robust, "dynamic" analysis multipotent hemopoietic progenitor cells undergoing self-renewal in response interleukin-3 (IL-3) multilineage differentiation lineage-affiliated cytokines. Cells...
Mucositis, a radiotherapy-associated toxicity, is an important factor determining morbidity and treatment compliance. Gastrointestinal mucositis in patients undergoing radiotherapy may also depend on time of administration radiation addition to several other factors. The presence any correlation between the severity acute gastrointestinal cervical carcinoma irradiation was prospectively evaluated.A total 229 with were randomized morning (8:00-10:00 AM) evening (6:00-8:00 PM) arms. incidence...
Loss-of-function mutations of EZH2, the enzymatic component PRC2, have been associated with poor outcome and chemotherapy resistance in T-cell acute lymphoblastic leukemia (T-ALL). Using isogenic T-ALL cells, without CRISPR/Cas9-induced EZH2-inactivating mutations, we performed a cell-based synthetic lethal drug screen. EZH2-deficient cells exhibited increased sensitivity to structurally diverse inhibitors CHK1, an interaction that could be validated genetically. Furthermore, small-molecule...
Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify surface antigen CD9 as a positive marker that provides simple alternative hematopoietic at high purity. Crucially, affords capture all cells in murine bone marrow absence contaminating populations lack authentic function. Using tool to subdivide stem-cell-containing populations, provide evidence heterogeneity cellular, functional, levels.
BackgroundThe reasons why some patients with COVID-19 develop pneumonia and others do not are unclear. To better understand this, we used multiparameter flow cytometry to profile circulating leukocytes from non-immunocompromised adult PCR-proven specifically compared those mild symptoms who had developed pneumonia.MethodsUsing clinically validated antibody panels studied 29 COVID-19. Ten were hypoxic requiring ventilatory support, eleven febrile but otherwise well, eight convalescing having...
Summary Monoclonal immunoglobulin M‐associated type I cryoglobulinaemia is poorly characterised. We screened 534 patients with monoclonal IgM disorders over a 9‐year period and identified 134 cryoglobulins. Of these, 76% had Waldenström macroglobulinaemia (WM), 5% other non‐Hodgkin lymphoma (NHL) 19% gammopathy of undetermined significance (MGUS). Clinically relevant IgM‐associated (including cold agglutinin disease [CAD], anti‐MAG antibodies, amyloidosis Schnitzler syndrome) coexisted in...