A5035000468- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Protist diversity and phylogeny
- Axon Guidance and Neuronal Signaling
- PARP inhibition in cancer therapy
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Immunodeficiency and Autoimmune Disorders
- Platelet Disorders and Treatments
- Endoplasmic Reticulum Stress and Disease
- Blood groups and transfusion
- Plant Molecular Biology Research
- Signaling Pathways in Disease
Biotest (Germany)
2023
Institute of Science and Technology Austria
2017-2020
Imperial College London
2017
The Francis Crick Institute
2016-2017
The Honourable Society of Lincoln's Inn
2012-2016
Cancer Research UK
2012-2014
European Molecular Biology Laboratory
2014
Microtubules are cytoskeleton filaments consisting of αβ-tubulin heterodimers. They switch between phases growth and shrinkage. The underlying mechanism this property, called dynamic instability, is not fully understood. Here, we identified a designed ankyrin repeat protein (DARPin) that interferes with microtubule assembly in unique manner. X-ray structure its complex GTP-tubulin shows it binds to the β-tubulin surface exposed at (+) ends. details provide insight into role GTP...
The function of microtubules relies on their ability to switch between phases growth and shrinkage. A nucleotide-dependent stabilising cap at microtubule ends is thought be lost before this can occur; however, the nature size protective are unknown. Using a microfluidics-assisted multi-colour TIRF microscopy assay with close-to-nm sub-second precision, we measured sizes stabilizing individual microtubules. We find that caps formed by extended binding regions EB proteins. Cap lengths vary...
Microtubules switch stochastically between phases of growth and shrinkage. The molecular mechanism responsible for the end a phase, an event called catastrophe, is still not understood. probability catastrophe to occur increases with microtubule age, putting constraints on possible induction. Here we used microfluidics-assisted fast tubulin washout experiments induce depolymerization in controlled manner at different times after start growth. We found that aging can also be observed this...
Significance Essential to microtubules’ function is their property of switching between phases growth and shrinkage, called dynamic instability. Growing microtubules are protected by the presence a GTP cap that thought fluctuate in size, causing stability fluctuations eventually result switch depolymerization. Despite importance these had not been quantitatively characterized until now, hence properties were previously unknown. Here we measure size develop theory explains based on kinetic...
Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed and shown to produce piconewton forces. However, some fungal are bidirectional. The force production a bidirectional has not yet been measured. Therefore, it remains unknown whether mechanism unconventional minus-end-directed motility differs fundamentally from that stepping. Using spectroscopy, we have measured here forces...
Human plasma is used for the generation of several life-saving drugs and contains valuable antibodies from immunoglobulin classes IgG, IgM IgA. Purified intravenous IgG solutions (IVIGs) form majority plasma-derived medicine to treat patients with various forms immunodeficiencies. In conventional IVIG manufacturing processes, IgA are often discarded as contaminants, but these antibody have been proven be effective treatment acute bacterial infections. Considering increase in demand human...
The differentiation of cells depends on a precise control their internal organization, which is the result complex dynamic interplay between cytoskeleton, molecular motors, signaling molecules, and membranes. For example, in developing neuron, protein ADAP1 (ADP-ribosylation factor GTPase-activating [ArfGAP] with dual pleckstrin homology [PH] domains 1) has been suggested to dendrite branching by regulating small GTPase ARF6. Together motor KIF13B, also thought mediate delivery second...