A5011691413- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Protist diversity and phylogeny
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- 14-3-3 protein interactions
- Plant Molecular Biology Research
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Protein Degradation and Inhibitors
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Micro and Nano Robotics
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Click Chemistry and Applications
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- interferon and immune responses
- Genetic and Kidney Cyst Diseases
- Mitochondrial Function and Pathology
Rockefeller University
2016-2025
Chitkara University
2023-2025
New York Proton Center
2014-2023
Tri-Institutional PhD Program in Chemical Biology
2023
Wake Forest University
2023
Hypertension Institute
2022
Delhi Technological University
2020
Chhatrapati Shahu Ji Maharaj University
2015
Protein Society
2012
Johannes Gutenberg University Mainz
2012
Small molecules that perturb specific protein functions are valuable tools for dissecting complex processes in mammalian cells. A combination of two phenotype-based screens, one based on a posttranslational modification, the other visualizing microtubules and chromatin, was used to identify compounds affect mitosis. One compound, here named monastrol, arrested cells mitosis with monopolar spindles. In vitro, monastrol specifically inhibited motility mitotic kinesin Eg5, motor required...
Monastrol, a cell-permeable small molecule inhibitor of the mitotic kinesin, Eg5, arrests cells in mitosis with monoastral spindles. Here, we use monastrol to probe mechanisms. We find that does not inhibit progression through S and G2 phases cell cycle or centrosome duplication. The arrest due is also rapidly reversible. Chromosomes monastrol-treated frequently have both sister kinetochores attached microtubules extending center monoaster (syntelic orientation). Mitotic arrest–deficient...
The stable propagation of genetic material during cell division depends on the congression chromosomes to spindle equator before initiates anaphase. It is generally assumed that requires are connected opposite poles bipolar ("bioriented"). In mammalian cells, we found can congress becoming bioriented. By combining use reversible chemical inhibitors, live-cell light microscopy, and correlative electron monooriented could glide toward alongside kinetochore fibers attached other already...
Near-simultaneous three-dimensional fluorescence/differential interference contrast microscopy was used to follow the behavior of microtubules and chromosomes in living α-tubulin/GFP-expressing cells after inhibition mitotic kinesin Eg5 with monastrol. Kinetochore fibers (K-fibers) were frequently observed forming association both during monastrol treatment removal. Surprisingly, these K-fibers oriented away from, not directly connected to, centrosomes incorporated into spindle by sliding...
During cell division metaphase spindles maintain constant length, whereas spindle microtubules continuously flux polewards, requiring addition of tubulin subunits at microtubule plus-ends, polewards translocation the lattice, and removal from minus-ends near poles. How these processes are coordinated is unknown. Here, we show that dynein/dynactin, a multi-subunit minus-end–directed motor complex, NuMA, cross-linker, regulate length. Fluorescent speckle microscopy reveals dynactin or NuMA...
T cells rapidly reposition their centrosome to the center of immunological synapse (IS) drive polarized secretion in direction bound target cell. Using an optical trap for spatial and temporal control over presentation, we show that repositioning Jurkat exhibited kinetically distinct polarization docking phases required calcium flux signaling through both cell receptor integrin be robust. In “frustrated” conjugates where is stuck behind nucleus, IS invaginated dramatically approach...