A5027378581- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Genomics and Phylogenetic Studies
- Reproductive Biology and Fertility
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Ubiquitin and proteasome pathways
- Chromatin Remodeling and Cancer
- Heat shock proteins research
- Sperm and Testicular Function
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
Waseda University
2020-2025
The Francis Crick Institute
2015-2022
The Honourable Society of Lincoln's Inn
2015
Tokyo University of Science
2009-2013
The University of Tokyo
2009-2013
Chiba University
2010
Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC's role chromatid correlates PCNA loading but is separable from its replication checkpoint. loads a slight preference for leading strand, which dispensable replication. Conversely,...
Abstract Underlying higher order chromatin organization are Structural Maintenance of Chromosomes (SMC) complexes, large protein rings that entrap DNA. The molecular mechanism by which SMC complexes organize is as yet incompletely understood. Two prominent models posit actively extrude DNA loops (loop extrusion), or they sequentially two DNAs come into proximity Brownian motion (diffusion capture). To explore the implications these mechanisms, we perform biophysical simulations a 3.76...
Abstract Background Structural maintenance of chromosomes (SMC) complexes are central organizers chromatin architecture throughout the cell cycle. The SMC family member condensin is best known for establishing long-range interactions in mitosis. These compact and create mechanically stable chromosomes. How contributes to organization interphase less well understood. Results Here, we use efficient conditional depletion fission yeast determine its contribution organization. We deplete...
Scientific Report21 August 2009Open Access Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast Masamitsu Sato Corresponding Author Laboratory Cell Regulation, Cancer Research UK, London Institute, 44 Lincoln's Inn Fields, London, WC2A 3PX UK Department Biophysics and Biochemistry, Graduate School Science, University Tokyo, 7-3-1 Hongo, 113-0033 Japan PRESTO, Science Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, 332-0012 Search for...
Integration of an external gene into a fission yeast chromosome is useful to investigate the effect product. An easy way knock-in construct use integration plasmid, which can be targeted and inserted through homologous recombination. Despite advantage integration, construction plasmids energy- time-consuming, because there no systematic library with various promoters, fluorescent protein tags, terminators selection markers; therefore, researchers are often forced make appropriate ones...
Abstract Motivation The 3D structure of chromatin in the nucleus is important for gene expression and regulation. Chromosome conformation capture techniques, such as Hi-C, generate large amounts data showing interaction points on genome but these are hard to interpret using standard tools. Results We have developed CSynth, an interactive browser real-time restraint-based modeller visualize models any chromosome (3C) data. Unlike other modelling systems, CSynth allows dynamic with parameters...
Abstract The progression of meiosis is controlled by a number gene‐expression systems in the fission yeast Schizosaccharomyces pombe . A forkhead‐type transcription factor Mei4 activates genes essential for from middle to late stages meiosis, which include I, II and sporulation. mei4 ‐deletion mutant ( Δ) arrests after meiotic prophase does not enter I. To further analyse function, we isolated novel temperature‐sensitive alleles. two alleles initial screen turned out contain substitution at...
Motile cilia of multiciliated epithelial cells undergo synchronized beating to produce fluid flow along the luminal surface various organs. Each motile cilium consists an axoneme and a basal body (BB), which are linked by "transition zone" (TZ). The exhibits characteristic 9+2 microtubule arrangement important for ciliary motion, but how this system is generated not yet fully understood. Here we show that calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), can stabilize minus-end...
Abstract The 3D structure of chromatin in the nucleus is important for gene expression and regulation. Chromosomal conformation capture techniques, such as Hi-C, generate large amounts data showing interaction points on genome but these are hard to interpret using standard tools. We have developed CSynth, a high performance browser real time restraint-based modeller visualise dynamic interactive models data. CSynth does its calculations GPU hence much faster than existing modelling software...
Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts to form condensed mitosis. Complete condensin inactivation necessary reveal how converts interphase into mitotic chromosomes. Here, we have developed a depletion system fission yeast combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient without need for temperature shift. Our useful...
Mitotic chromosomes give genome portions the required compaction and mechanical stability for faithful inheritance during cell divisions. Here, we record human chromosome dimensions from their appearance in prophase over successive times a mitotic arrest. Chromosomes first appear long uniformly thin. Then, individual arms become discernible, which continuously shorten thicken - longer arm, thicker it becomes. The observed arm length to width relationship can be described by power law with...
Abstract Underlying higher order chromatin organization are Structural Maintenance of Chromosomes (SMC) complexes, large protein rings that entrap DNA. The molecular mechanism by which SMC complexes organize is as yet incompletely understood. Two prominent models posit actively extrude DNA loops (loop extrusion), or they sequentially two DNAs come into proximity Brownian motion (diffusion capture). To explore the implications these mechanisms, we perform biophysical simulations a 3.76...
Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts to form condensed mitosis. Complete condensin inactivation necessary reveal how converts interphase into mitotic chromosomes. Here, we have developed a depletion system fission yeast combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient without need for temperature shift. Our useful...
Abstract Motile cilia of multiciliated epithelial cells undergo synchronized beating to produce fluid flow along the luminal surface various organs. Each motile cilium consists an axoneme and a basal body, which are linked by ‘transition zone’. The exhibits characteristic 9+2 microtubule arrangement important for ciliary motion, but how this system is generated not yet fully understood. Here we show that CAMSAP3, protein can stabilize minus end microtubule, concentrates at multiple sites...