A5006821638- Genetic and Kidney Cyst Diseases
- Fusion materials and technologies
- Advancements in Solid Oxide Fuel Cells
- Microtubule and mitosis dynamics
- Nuclear reactor physics and engineering
- Nuclear Physics and Applications
- Fuel Cells and Related Materials
- Nuclear Materials and Properties
- Particle accelerators and beam dynamics
- Electrocatalysts for Energy Conversion
- Protist diversity and phylogeny
- Spacecraft and Cryogenic Technologies
- Muon and positron interactions and applications
- Superconducting Materials and Applications
- Magnetic confinement fusion research
- Laser Material Processing Techniques
- Non-Destructive Testing Techniques
- Chemical Synthesis and Characterization
- Welding Techniques and Residual Stresses
- Hydrogen Storage and Materials
- Photosynthetic Processes and Mechanisms
- Crystallography and molecular interactions
- Radioactive contamination and transfer
- Field-Flow Fractionation Techniques
- Advanced Materials Characterization Techniques
Tokyo Gas (Japan)
2000-2021
RIKEN Center for Biosystems Dynamics Research
2020-2021
The University of Tokyo
2009-2021
Tokyo University of the Arts
2021
Life Science Institute
2016
Tokyo Institute of Technology
2016
Tokyo University of Science
1994-2015
National Institute of Infectious Diseases
2012
Sumitomo Heavy Industries (Japan)
1977-2003
Japan Atomic Energy Agency
2003
Three complete cDNA clones encoding Id-related helix-loop-helix (HLH) proteins lacking a basic region were isolated from pcD2 expression library prepared TIG-3 human diploid fibroblasts (HDF). Of these cDNAs (Id-1H, Id-1H', and Id-2H), two (Id-1H Id-1H') appeared to be derived by alternative RNA splicing. Id-1H Id-2H seem homologues of mouse Id-1 Id-2, respectively, have potential encode 154 135 amino acid proteins. The mRNAs barely detectable in quiescent early passage HDF; serum...
Significance The phototactic behavior of the unicellular green alga Chlamydomonas reinhardtii is thought to rely on photoreception by eyespot apparatus. Here, we isolated an eyespot-less mutant that clearly exhibits phototaxis. Intriguingly, sign (the direction cell migration) in this opposite wild type after treatment with reagents enhance sign, a property also detected previously reported mutants. reversed phototactic-sign phenotype was attributed fact photoreceptors were exposed condensed...
Abstract Axonemal dynein ATPases direct ciliary and flagellar beating via adenosine triphosphate (ATP) hydrolysis. The modulatory effect of monophosphate (AMP) diphosphate (ADP) on is not fully understood. Here, we describe a deficiency cilia flagella associated protein 45 ( CFAP45 ) in humans mice that presents motile ciliopathy featuring situs inversus totalis asthenospermia. CFAP45-deficient show normal morphology axonemal ultrastructure. Proteomic profiling links to an module including...
Left-right (L-R) asymmetric intraciliary calcium transient at the node is determinant of L-R axis in mice.
ABSTRACT Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, is a major virion tegument protein. In present study, we showed that VP22 was required for efficient redistribution of viral proteins VP16, VP26, ICP0, ICP4, and ICP27 cellular Hsc-70 to cytoplasm infected cells. We found two dileucine motifs in at amino acids 235 236 251 252, were necessary regulation proper cytoplasmic localization these proteins. The also itself optimal expression UL41, glycoprotein B....
Cytoplasmic assembly of ciliary dyneins, a process known as preassembly, requires numerous non-dynein proteins, but the identities and functions these proteins are not fully elucidated. Here, we show that classical Chlamydomonas motility mutant pf23 is defective in homolog DYX1C1. The has 494 bp deletion DYX1C1 gene expresses shorter protein cytoplasm. Structural analyses, using cryo-ET, reveal axonemes lack most inner dynein arms. Spectral counting confirms essential for majority arms (IDA)...
The unicellular green alga Chlamydomonas reinhardtii is a model organism for various studies in biology. CC-124 laboratory strain widely used as wild type. However, this known to carry agg1 mutation, which causes cells swim away from the light source (negative phototaxis), contrast of other wild-type strains, toward (positive phototaxis). Here we identified causative gene (AGG1) using AFLP-based mapping and whole genome next-generation sequencing. This encodes 36-kDa protein containing...
Motile cilia can beat with distinct patterns, but how motility variations are regulated remain obscure. Here, we have studied the role of coiled-coil protein CFAP53 in different cilia-types mouse. While node (9+0) Cfap53 mutants were immotile, tracheal and ependymal (9+2) retained motility, albeit an altered pattern. In cilia, mainly localized at base (centriolar satellites), whereas it was also present along entire axoneme cilia. associated tightly microtubules interacted axonemal dyneins...
Abstract Flagellar beating in Chlamydomonas was found to be activated by mechanical stimulation. Immediately after a wild‐type cell suspension vortexed, the average swimming velocity of cells increased from 130 μm/second 150 μm/second, due an elevation flagellar beat frequency ∼60 Hz ∼70 without detectable change waveforms. This response required outer arm dynein. Treatment with EGTA, Ca 2+ ‐channel blockers, or mechanosensitive‐channel blockers inhibited it. In demembranated and reactivated...
Abstract Achiral ethylenediamine forms chiral structures spontaneously when crystallized as the sulfate salt. By using these crystals of (ethylenediammonium sulfate), asymmetric autocatalysis pyrimidyl alkanol affords a highly enantioenriched product with chirality that is determined by crystals.
An experiment on the separation of hydrogen isotopes has been carried out by using a thermal diffusion column with “cryogenic-wall” cooled liquid nitrogen. The factor was compared that ordinary water, and for is higher than “water wall” column. Moreover, obtained 473 K operation hot wire in system found to be greater 1073 operation. Probably because isotopic exchange reaction between H2 D2 suppressed operation; there no HD component observed this case, while an equilibrium amount immediately detected
Free-exciton recombination emission of 235 nm in wavelength is obtained by current injection at room temperature from a diamond-based pn junction diode composed B-doped crystal grown high-temperature, high-pressure synthesis and S-doped homoepitaxial layer the chemical vapor deposition method. The shows clear rectification characteristic high external quantum efficiency excitonic emission, 8×10 -5 , which indicates that diamond good candidate for application to semiconductor...
Outer arm dynein (OAD) is bound to specific loci on outer-doublet-microtubules by interactions at two sites: via intermediate chain 1 (IC1) and the outer docking complex (ODA-DC). Studies using Chlamydomonas mutants have suggested that individual sites rather weak affinities for microtubules, therefore strong OAD attachment microtubules achieved their cooperation. To test this idea, we examined between IC1, IC2 (another chain) ODA-DC recombinant proteins. Recombinant IC1 were found form a...
At the Tritium Process Laboratory (TPL) in Japan Atomic Energy Research Institute, Thermal Diffusion process is applied to enrich and recover tritium diluted by operation experiments of other apparatus installed gloveboxes. The required 200-liter hydrogen isotope mixture containing up lg batch 90%. Preliminary studies were performed numerically as well experimentally, good agreement was obtained. Based on results, designed. Major components are four 2.5m-high diffusion columns, reservoirs,...
Abstract A solid oxide fuel cell (SOFC) module using two‐stage stacks and a regeneration process between them was developed in this study for the first time, to best of authors' knowledge. Upon configuring first‐stage second‐stage steam reformer SOFC module, gross output power DC 2.27 kW generated with generation efficiency 69.2% (lower heating value (LHV)), at total utilization rate 86.3%. This technology enables operation very high even while operating moderate (below 70%). Considering an...
We have examined the role of Fam60a, a gene highly expressed in embryonic stem cells, mouse development. Fam60a interacts with components Sin3a-Hdac transcriptional corepressor complex, and most Fam60a–/– embryos manifest hypoplasia visceral organs die utero. is recruited to promoter regions subset genes, expression these genes being either up- or down-regulated embryos. The DNA methylation level target Adhfe1 maintained at day (E) 7.5 but markedly reduced E9.5 embryos, suggesting that...
Motile cilia of multiciliated epithelial cells undergo synchronized beating to produce fluid flow along the luminal surface various organs. Each motile cilium consists an axoneme and a basal body (BB), which are linked by "transition zone" (TZ). The exhibits characteristic 9+2 microtubule arrangement important for ciliary motion, but how this system is generated not yet fully understood. Here we show that calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), can stabilize minus-end...
Click to increase image sizeClick decrease sizeKEYWORDS: thermal diffusion columncryogenic wallliquid nitrogenisotope separationsseparation factortemperature dependencetritiumdeuterium
To improve the power generation efficiency of a solid oxide fuel cell (SOFC) system, we tried to operate system at extremely high utilization around 90% while keeping lower for individual cell-stacks. We designed with two-stage SOFC stacks and regeneration process. The was operated total rate 88.4%, gross reached DC 71.2% its heating value output 2 kW. In addition, our more than 500 hours without degradation. technologies process were proven make stable possible even in system.
Two-stage stack technology is a method to generate highly efficient power using fuel cells. To achieve power, it important regenerate anode gas between the two-stage stacks. The effect on generation efficiency by removing H2O and/or CO2 from regenerator has been investigated simulating solid oxide cell model. risk of carbon deposition regenerated also calculating equilibrium state gas. maximum net AC 71.8% (lower heating value) was obtained when and were 100% removed gas, assuming that...
Tokyo Gas has developed a highly efficient solid oxide fuel cell system by increasing the utilization rate using two-stage stacks and regeneration. We focused on separation membrane, which removes both steam carbon dioxide from anode off-gas of first-stage stack. In this study, we evaluated gas properties membrane module. connected module to our fifth-generation hot power generation performance. The results show DC efficiency 74.8%LHV at gross output 4.89 kW. addition, applied separate...
We have developed a multi-layer eddy current micro sensor. The dimension of the sensor is 3mm x 80 pm. It was composed one to four layers spiral coil made 10 pm2 copper lines. number turn in each layer 40 turns. In present work, we report on fabrication and evaluation sensors both experiments 3D FEM analysis.