A5047353113- Advanced Proteomics Techniques and Applications
- Chemical Synthesis and Analysis
- Mass Spectrometry Techniques and Applications
- Genomics and Phylogenetic Studies
- Neuropeptides and Animal Physiology
- Marine Ecology and Invasive Species
- Peptidase Inhibition and Analysis
- Planarian Biology and Electrostimulation
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Hypothalamic control of reproductive hormones
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- RNA Research and Splicing
- Esophageal and GI Pathology
- Homotopy and Cohomology in Algebraic Topology
- Monoclonal and Polyclonal Antibodies Research
- Tracheal and airway disorders
- Genomics and Chromatin Dynamics
- Neurogenesis and neuroplasticity mechanisms
- Regulation of Appetite and Obesity
- Cardiac and Coronary Surgery Techniques
- Apelin-related biomedical research
- Protein purification and stability
RIKEN Center for Biosystems Dynamics Research
2018-2023
RIKEN
2023
CLS Technology (Norway)
2018-2023
Shumei University
2022
National Institute of Genetics
2021
Kyoto University
2009-2020
Community Medical Center
2019
CE Technologies (United Kingdom)
2018
Osaka University
2007-2017
Protein Research Foundation
2006-2017
The β-chemokine receptor CCR5 is considered to be an attractive target for inhibition of macrophage-tropic (CCR5-using or R5) HIV-1 replication because individuals having a nonfunctional (a homozygous 32-bp deletion in the coding region) are apparently normal but resistant infection with R5 HIV-1. In this study, we found that TAK-779, nonpeptide compound small molecular weight ( M r 531.13), antagonized binding RANTES (regulated on activation, T cell expressed and secreted) CCR5-expressing...
We report a new web server, aLeaves (http://aleaves.cdb.riken.jp/), for homologue collection from diverse animal genomes. In molecular comparative studies involving multiple species, orthology identification is the basis on which most subsequent biological analyses rely. It can be achieved accurately by explicit phylogenetic inference. More and more species are subjected to large-scale sequencing, but resultant resources scattered in independent project-based, multi-species, separate, sites....
We have recently identified apelin as the endogenous ligand for human APJ. In rats, highest expression of APJ mRNA was detected in lung, suggesting that and its play an important role pulmonary system. When apelin-36 pyroglutamylated C-terminal peptide, [<Glu65]apelin-13, were compared microphysiometric analyses, elevation extracellular acidification induced cells expressing by [<Glu65]apelin-13 transient, whereas sustained. These responses almost completely inhibited a specific inhibitor Gi...
Along with the increasing accessibility to comprehensive sequence information, such as whole genomes and transcriptomes, demand for assessing their quality has been multiplied. To this end, metrics based on lengths, N50, have become a standard, but they only evaluate one aspect of assembly quality. Conversely, analyzing coverage pre-selected reference protein-coding genes provides essential content-based assessment, currently available pipelines purpose, CEGMA BUSCO, do not user-friendly...
Modern cartilaginous fishes are divided into elasmobranchs (sharks, rays and skates) chimaeras, the lack of established whole-genome sequences for former has prevented our understanding early vertebrate evolution unique phenotypes elasmobranchs. Here we present de novo assemblies brownbanded bamboo shark cloudy catshark an improved assembly whale genome. These relatively large genomes (3.8-6.7 Gbp) contain sparse distributions coding genes regulatory elements exhibit reduced molecular...
Airway hyperresponsiveness (AHR), goblet cell metaplasia, and mucus overproduction are important features of bronchial asthma. To elucidate the molecular mechanisms behind these pulmonary pathologies, we examined for genes preferentially expressed in lungs a murine model allergic asthma by using suppression subtractive hybridization (SSH). We identified gene called gob-5 that had selective expression pattern airway epithelium with AHR. Here, show gob-5, member calcium-activated chloride...
Hydrolysis of the meningococcal group A, B, and C, Escherichia coli K92 polysaccharides by 60% aqueous hydrofluoric acid liberated various 1,2-diacylglycerols.These were extracted with chloroform, trimethylsilylated, analyzed gas chromatography-mass spectrometry.Two 1,2-diacylglycerols major components isolated.In each polysaccharide, 80 to 90% dipalmitoyl glycerol 10 20% distearoyl identified.No monoacylglycerols or mixed diacyglycerols noted.The presence hydrophobic end causes aggregate in...
Neuromedin U is a bioactive peptide first isolated from porcine spinal cord. In this paper, we demonstrate that neuromedin the cognate ligand for orphan G protein-coupled receptor, FM-3, originally as homologue of neurotensin and growth hormone secretogogue receptors. induced specific evident elevation extracellular acidification rates, arachidonic acid metabolite release, intracellular Ca2+mobilization in Chinese hamster ovary cells expressing human FM-3. addition, radiolabeled specifically...
The robust regenerative ability of planarians depends on a population somatic stem cells called neoblasts, which are the only mitotic in adults and responsible for blastema formation after amputation. molecular mechanism underlying neoblast differentiation associated with remains unknown. Here, using planarian Dugesia japonica we found that DjmkpA, mitogen-activated protein kinase (MAPK) phosphatase-related gene, was specifically expressed response to increased extracellular signal-related...
Comparative genome analysis of non-avian reptiles and amphibians provides important clues about the process evolution in tetrapods. However, there is still only limited information available on structures these organisms. Consequently, protokaryotypes amniotes tetrapods evolutionary processes microchromosomes remain poorly understood. We constructed chromosome maps functional genes for Chinese soft-shelled turtle (Pelodiscus sinensis), Siamese crocodile (Crocodylus siamensis), Western clawed...
The identification of molecular networks at the system level in mammals is accelerated by next-generation mammalian genetics without crossing, which requires both efficient production whole-body biallelic knockout (KO) mice a single generation and high-performance phenotype analyses. Here, we show that triple targeting gene using CRISPR/Cas9 achieves almost perfect KO efficiency (96%-100%). In addition, developed respiration-based fully automated non-invasive sleep phenotyping system, Snappy...
The majority of non-coding RNAs (ncRNAs) involved in mRNA metabolism mammals have been believed to downregulate the corresponding expression level a pre- or post-transcriptional manner by forming short long ncRNA-mRNA duplex structures. Information on non-duplex-forming ncRNAs is now also rapidly accumulating. To examine directional properties transcription at whole-genome level, we performed RNA-seq analysis mouse and chimpanzee tissue samples.We found that there only about 1% genome where...
The molecular etiology of idiopathic pulmonary fibrosis (IPF) has been extensively investigated to identify new therapeutic targets. Although anti-inflammatory treatments are not effective for patients with IPF, damaged alveolar epithelial cells play a critical role in lung fibrogenesis. Here, we establish an organoid-based model using mouse and human tissues assess the direct communication between type II (AT2)-lineage fibroblasts by excluding immune cells. Using this vitro genetics,...
The search for new small-molecule CCR5 antagonists by high-throughput screening (HTS) of the Takeda chemical library using [(125)I]RANTES and CHO/CCR5 cells led to discovery lead compounds (A, B) with a quaternary ammonium or phosphonium moiety, which were synthesized investigate MCP-1 receptor antagonists. A series novel anilide derivatives 1 moiety designed, synthesized, tested their antagonistic activity. Through optimization compounds, we have found...