A5052996512- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Hydrogen's biological and therapeutic effects
- RNA Research and Splicing
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Gene expression and cancer classification
- Immune Cell Function and Interaction
- Animal Genetics and Reproduction
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Cancer-related gene regulation
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Biochemical effects in animals
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- RNA modifications and cancer
- bioluminescence and chemiluminescence research
- Epigenetics and DNA Methylation
National Institutes for Quantum Science and Technology
1998-2024
Tohoku University
2024
Cell Biotech (South Korea)
2017
Kyushu University
1967-2014
National Research Institute of Police Science
2011-2014
Panasonic (Japan)
2005
NTL Institute for Applied Behavioral Science
2003
Radiation Effects Research Foundation
1989-1997
University Clinic of Traumatology
1990
Kyoto University
1990
The severe combined immune deficiency (SCID) mouse was reported as an animal model for human deficiency. Through the course of several studies, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) gene came to be considered a candidate SCID-responsible gene. We isolated ORF murine DNA-PKcs from SCID mice and their parent strain C.B-17 determined DNA sequences. contained 4128-aa residues had 78.9% homology with A particularly important finding is that T transversion results in...
Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive their scientific medical utilities might prove limited. To evaluate characteristics of NT-ESCs, we established more than 150 NT-ESC lines adult somatic several mouse strains. Here, show that these were able...
The robust regenerative ability of planarians depends on a population somatic stem cells called neoblasts, which are the only mitotic in adults and responsible for blastema formation after amputation. molecular mechanism underlying neoblast differentiation associated with remains unknown. Here, using planarian Dugesia japonica we found that DjmkpA, mitogen-activated protein kinase (MAPK) phosphatase-related gene, was specifically expressed response to increased extracellular signal-related...
Two major complementary double-strand break (DSB) repair pathways exist in vertebrates, homologous recombination (HR), which involves Rad54, and non-homologous end-joining, requires the DNA-dependent protein kinase (DNA-PK). DNA-PK comprises a catalytic subunit (DNA-PKcs) DNA-binding Ku70 Ku80 heterodimer. To define activities of individual components DSB repair, we targeted DNA-PKcs gene chicken DT40 cells. deficiency caused defect that was, unexpectedly, suppressed by<i>KU70</i>...
Neighboring genes are often coordinately expressed within cis-regulatory modules, but evidence that nonparalogous share functions in mammals is lacking. Here, we report mutation of either TMEM138 or TMEM216 causes a phenotypically indistinguishable human ciliopathy, Joubert syndrome. Despite lack sequence homology, the aligned head-to-tail configuration and joined by chromosomal rearrangement at amphibian-to-reptile evolutionary transition. Expression two mediated conserved regulatory...
Point mutations at codons 12 and 13 of c-Ki-ras gene were analyzed in human pancreatic cancer. DNAs obtained from sample tissues amplified by means polymerase chain reaction dot blot hybridization assays with oligonucleotide probes appropriate for detecting these codons. Out 38 evaluated cases, point codon found 35 cases; resulted changes the coded amino acid to aspartic 24 valine 9 arginine 2 cases cysteine one case. In case, a glycine-to-aspartic mutation was 13. two distinct...
Vascular endothelial growth factor (VEGF) is a key mediator of tumor angiogenesis. Tumor cells are exposed to higher oxidative stress compared normal cells. Numerous reports have demonstrated that the intracellular redox (oxidation/reduction) state closely associated with pattern VEGF expression. Electrolyzed reduced water (ERW) produced near cathode during electrolysis scavenged H2O2 and decreased release from human lung adenocarcinoma cell line, A549, down-regulated both transcription...
A large number of point mutations have been identified in induced pluripotent stem cell (iPSC) genomes to date. Whether these are associated with iPSC generation is an important and controversial issue. In this study, we approached critical issue different ways, including assessment iPSCs versus embryonic cells (ESCs), investigation variant allele frequencies the heterogeneity within a single clone. Through analyses, obtained strong evidence that iPSC-generation-associated occur frequently...
Induced pluripotent stem cells (iPSCs) are generated by direct reprogramming of somatic and hold great promise for novel therapies. However, several studies have reported genetic variations in iPSC genomes. Here, we investigated point mutations identified whole-genome sequencing mouse human iPSCs the context epigenetic status. In contrast to disease-causing single-nucleotide polymorphisms, de novo introduced during were underrepresented protein-coding genes open chromatin regions, including...
The effect of space radiation on offspring was evaluated using mouse freeze-dried spermatozoa preserved the Space Station.
c-Myc transduction has been considered previously to be nonessential for induced pluripotent stem cell (iPSC) generation. In this study, we investigated the effects of on generation iPSCs from an inbred mouse strain using a genome integration-free vector exclude genetic background and genomic integration exogenous genes. Our findings reveal clear difference between generated four defined factors including (4F-iPSCs) those produced without (3F-iPSCs). Molecular cellular analyses did not any...
A number of point mutations have been identified in reprogrammed pluripotent stem cells such as iPSCs and ntESCs. The molecular basis for these has remained elusive however, which is a considerable impediment to their potential medical application. Here we report specific stage at iPSC generation not reduced response ionizing radiation, i.e. radio-resistance. Quite intriguingly, G1/S cell cycle checkpoint deficiency occurs transient fashion the initial genome reprogramming process. These...
Background Dendritic cells (DCs) are a promising therapeutic target in cancer immunotherapy given their ability to prime antigen-specific T cells, and initiate antitumor immune response. A major obstacle for DC-based is the difficulty obtain sufficient number of functional DCs. Theoretically, this limitation can be overcome by using induced pluripotent stem (iPSCs); however, strategies engage iPSC-derived DCs (iPSC-DCs) into remain elucidated. Accumulating evidence showing that induction...
Phosphorylation at serine 15 of the human p53 tumor suppressor protein is induced by DNA damage and correlates with accumulation its activation as a transcription factor. The DNA-dependent kinase (DNA-PK) can phosphorylate homologous 18 murine in vitro. Contradictory reports exist about requirement for DNA-PK vivo cell cycle arrest response to ionizing radiation. While primary SCID (severe combined immunodeficiency) cells, that have defective DNA-PK, show normal arrest, transcriptionally...
The neoblasts are the only somatic stem cells in planarians possessing pluripotency, and can give rise to all types of cells, including germline cells. Recently, accumulated knowledge about transcriptome expression dynamics various pluripotent has provided important opportunities understand not fundamental mechanisms but also stemness across species at molecular level. easily be eliminated by radiation. Also, using fluorescence activated cell sorting (FACS), we purify collect many neoblasts,...
The variable region of immunoglobulin (Ig) heavy chain is encoded by three separate genes: (VH), diversity (DH) and joining (JH) genes on the germ-line genome. In mice, most complementarity determining (CDR) III chains myelomas hybridomas sequenced so far can be assigned to one 12 already identified DH homology nucleotide sequences gene-coding regions although extranucleotides, so-called N segments, are found at boundaries between JH as well VH DH. On other hand, Siebenlist et al. (Nature...
ABSTRACT Koala retrovirus (KoRV) is a gammaretrovirus that currently endogenizing into koalas. Studies on KoRV infection have been hampered by the lack of replication-competent molecular clone. In this study, we constructed an infectious clone, termed plasmid pKoRV522, isolate (strain Aki) from koala reared in Japanese zoo. The virus KoRV522, derived grew efficiently human embryonic kidney (HEK293T) cells, attaining 10 6 focus-forming units/ml. Several mutations Gag (L domain) and Env...
Abstract Unlike most DNA-dependent protein kinase, catalytic subunit (DNA-PKcs)–deficient mouse cell strains, we show in the present study that targeted deletion of DNA-PKcs two different human lines abrogates VDJ signal end joining episomal assays. Although mechanism is not well defined, deficency results spontaneous reduction ATM expression many cultured (including those examined this study) and DNA-PKcs–deficient mice. We considered varying loss might explain differences strains animal...
Human Tetherin/BST-2 has recently been identified as a cellular antiviral factor that blocks the release of various enveloped viruses. In this study, we cloned cDNA fragment encoding feline homolog and characterized protein product. The degree amino acid sequence identity between human was 44.4%. Similar to Tetherin/BST-2, expression mRNA inducible by type I interferon (IFN). Exogenous efficiently inhibited endogenous retrovirus RD-114. extracellular domain two putative N-linked...
We established the radiosensitive cell line SX9 from mammary carcinoma FM3A. In cells a defect of DNA-dependent protein kinase (DNA-PK) activity was suggested. Additionally, complementation test suggested that belongs to x-ray cross-complementing group (XRCC) 7. Isolation and sequence analyses catalytic subunit <i>(dna-pkcs)</i> cDNA in disclosed nucleotide "T" (9572) "C" transition causing substitution amino acid residue leucine (3191) proline. Interestingly, mutation occurs one allele,...