A5010481557- Planarian Biology and Electrostimulation
- Marine Ecology and Invasive Species
- Plant and Biological Electrophysiology Studies
- Marine Invertebrate Physiology and Ecology
- Developmental Biology and Gene Regulation
- bioluminescence and chemiluminescence research
- Neuroscience and Neural Engineering
- Animal Genetics and Reproduction
- Silk-based biomaterials and applications
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Antimicrobial Peptides and Activities
- Retinal Development and Disorders
- Cephalopods and Marine Biology
- Advanced Scientific Research Methods
- Ichthyology and Marine Biology
- Insect and Arachnid Ecology and Behavior
- Neurogenesis and neuroplasticity mechanisms
- Photochromic and Fluorescence Chemistry
- Marine Biology and Environmental Chemistry
- Fish Biology and Ecology Studies
- Retinoids in leukemia and cellular processes
- Parasite Biology and Host Interactions
- Cell Adhesion Molecules Research
University of Hyogo
2015-2023
Kyoto University
1999-2022
Tokushima University
2014
Google (United States)
2008
Himeji University
1998-2003
Institut thématique Génétique, génomique et bioinformatique
2002
Japan Science and Technology Agency
2002
Kobe University
1994-1999
Nara Institute of Science and Technology
1999
Hyogo Prefectural Institute of Technology
1999
Planarians are considered to be among the most primitive animals which developed central nervous system (CNS). To understand origin and evolution of CNS, we have isolated a neural marker gene from planarian, Dugesia japonica, analyzed structure planarian CNS by in situ hybridization. The is located on ventral side body, composed mass cephalic ganglions head region pair nerve cords (VNC). Cephalic cluster independently VNC, more dorsal than form an inverted U-shaped brain-like with nine...
Nuclear receptors comprise a large and expanding family of transcription factors involved in diverse aspects animal physiology development, the functions which can be modulated spatial temporal manner by access to small lipophilic ligands and/or specificity their own localized expression. Here we report identification human nuclear receptor that reveals unique proximal box (CNGCSG) DNA-binding domain. The conservation this feature its nematode counterpart suggests requirement for type P...
To analyze the organization of planarian brain, a homolog homeobox‐containing gene Orthopedia (Otp) from planarian, Djotp , was isolated. The homeodomain differs mouse Otp by only two amino acids. This conservation extends to include 12 acid motif downstream homeodomain. Whole mount in situ hybridization studies indicated that is specifically expressed branch structures normal adult brain. During regeneration, presumptive region prior formation. These observations implicate role for...
Despite long-standing interest, the molecular mechanisms underlying establishment of anterior-posterior (AP) polarity remain among unsolved mysteries in metazoans. In planarians (a family flatworms), canonical Wnt/beta-catenin signaling is required for posterior specification, as it many animals. However, regulating posterior-specific induction Wnt genes according to AP have remained unclear. Here, we demonstrate that Hedgehog (Hh) responsible via its regulation transcription during...
The robust regenerative ability of planarians depends on a population somatic stem cells called neoblasts, which are the only mitotic in adults and responsible for blastema formation after amputation. molecular mechanism underlying neoblast differentiation associated with remains unknown. Here, using planarian Dugesia japonica we found that DjmkpA, mitogen-activated protein kinase (MAPK) phosphatase-related gene, was specifically expressed response to increased extracellular signal-related...
When exposed to light, planarians display a distinctive light avoidance behavior known as negative phototaxis. Such is temporarily suppressed when animals are decapitated, and it restored once the regenerate their heads. Head regeneration simple but reproducible phototactic response of provides an opportunity study association between neuronal differentiation establishment in simple, experimentally tractable metazoan. We have devised phototaxis assay system analyze recovery during head...
Abstract Planarian, an invertebrate flatworm, has a high capacity for regeneration when compared with other worms and animals. We show here the first time that reconstructed dopamine (DA) neural network regulates locomotion behavior in planarian regenerates. The gene encoding tyrosine hydroxylase Dugesia japonica ( DjTH ) was identified. protein coexpressed aromatic amino acid decarboxylase‐like A (DjAADCA) central nervous system (CNS). In addition, ‐knockdown planarians lost ability to...
The robust regenerative abilities of planarians absolutely depend on a unique population pluripotent stem cells called neoblasts, which are the only mitotic somatic in adult and responsible for blastema formation after amputation. Little is known about molecular mechanisms that drive during planarian regeneration. Here we found treatment with c‐Jun N‐terminal kinase (JNK) inhibitor SP600125 blocked entry neoblasts into M‐phase cell cycle, while allowing to successfully enter S‐phase Dugesia...
We established a laboratory clonal strain of freshwater planarian (Dugesia japonica) that was derived from single individual and continued to undergo autotomous asexual reproduction for more than 20 years, we performed large-scale genome sequencing transcriptome analysis on it. Despite the fact completely used, an unusually large number mutations were detected. To enable quantitative genetic such unique organism, developed new model called Reference Gene Model, used it conduct analysis. The...
We established a novel knock-in technique, New and Easy Xenopus Targeted integration (NEXTi), to recapitulate endogenous gene expression by reporter expression. NEXTi is CRISPR-Cas9-base method integrate donor DNA containing (egfp) into target 5' UTR of laevis genome. It enables us track eGFP under regulation promoter/enhancer activities. obtained about 2% 13% embryos showing signal in tissue-specific manner, targeting krt.12.2.L, myod1.S, sox2.L bcan.S loci, as previously reported. In...
Freshwater planarians have a simple and evolutionarily primitive brain structure. Here, we identified the Djsnap‐25 gene encoding homolog of conserved synaptic protein SNAP‐25 from planarian Dugesia japonica assessed its role in function. was expressed widely nervous system. To investigate specific brain, developed unique technique RNA interference (RNAi), regeneration‐dependent conditional knockdown (Readyknock), exploiting high regenerative capacity planarians, succeeded selectively...
Planarians have strong regenerative abilities derived from their adult pluripotent stem cell (neoblast) system. However, the molecular mechanisms involved in planarian regeneration long remained a mystery. In particular, no anterior-specifying factor(s) could be found, although Wnt family proteins had been successfully identified as posterior-specifying factors during (Gurley et al., 2008; Petersen and Reddien, 2008). A recent textbook of developmental biology therefore proposes antagonist...