A5072942253- Monoclonal and Polyclonal Antibodies Research
- Telomeres, Telomerase, and Senescence
- Biochemical effects in animals
- Viral Infectious Diseases and Gene Expression in Insects
- Hydrogen's biological and therapeutic effects
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Protein purification and stability
- Cancer Research and Treatments
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Sirtuins and Resveratrol in Medicine
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Advanced biosensing and bioanalysis techniques
- Probiotics and Fermented Foods
- Stress Responses and Cortisol
- Medicinal Plants and Bioactive Compounds
- Enzyme Production and Characterization
- Food Allergy and Anaphylaxis Research
- CAR-T cell therapy research
- Genetics, Aging, and Longevity in Model Organisms
- Proteins in Food Systems
- Cytomegalovirus and herpesvirus research
Kyushu University
2015-2024
Life Systems (United States)
2016
Center for Systems Biology
1994-2013
Genetic Resources Center
2009
The University of Tokyo
1994-2003
Tokyo University of Agriculture
1994
Tokyo University of Agriculture and Technology
1993
Agricultural & Applied Economics Association
1990
Our goal in this study was to determine whether or not anserine/carnosine supplementation (ACS) is capable of preserving cognitive function elderly people. In a double-blind randomized controlled trial, volunteers were randomly assigned an ACS placebo group at 1:1 ratio. The took 1.0 g (3:1) formula daily for 3 months. Participants evaluated by psychological tests before and after the 3-month period. Thirty-nine healthy (60-78 years old) completed follow-up tests. Among tests, delayed recall...
A system is developed to magnetically measure biological antigen-antibody reactions with a superconducting quantum interference device (SQUID) magnetometer. In this system, antibodies are labeled magnetic nanoparticles of γ-Fe 2 O 3 , and the measured by detecting field from nanoparticles. setup described, sensitivity studied in terms detectable weight Magnetic particles as small 600 pg can be detected at present. An experiment also conducted reaction present system. It shown that better...
Vascular endothelial growth factor (VEGF) is a key mediator of tumor angiogenesis. Tumor cells are exposed to higher oxidative stress compared normal cells. Numerous reports have demonstrated that the intracellular redox (oxidation/reduction) state closely associated with pattern VEGF expression. Electrolyzed reduced water (ERW) produced near cathode during electrolysis scavenged H2O2 and decreased release from human lung adenocarcinoma cell line, A549, down-regulated both transcription...
Our goal was to determine whether anserine/carnosine supplementation (ACS) suppresses chemokine levels in elderly people. In a double-blind randomized controlled trial, volunteers were assigned the ACS or placebo group (1:1). Sixty healthy (active, n = 30; placebo, 30) completed study. The administered 1.0 g of (3:1) for 3 months. A microarray analysis and subsequent quantitative real-time polymerase chain reaction (qRT-PCR) peripheral blood mononuclear cells (PBMCs) showed decreased...
Epidermal growth factor receptor (EGFR) is highly expressed in several types of cancer cells including oral squamous cell carcinoma (OSCC). EGF/EGFR signaling recognized as an important molecular target therapy. However, often become tolerant to signaling-targeted therapies. In the tumor microenvironment, incites inflammation and inflammation-derived cytokines make a considerable impact on development. addition, hyperosmolarity also induced, but role osmotic stress development has not been...
Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using system screening food components that activate the TERT promoter HaCaT cells (keratinocytes). In present study, aimed to clarify molecular basis of fisetin-induced promotion mice. To this end, dorsal skin mice was treated with fisetin, and evaluated 12 days after treatment. Histochemical analyses fisetin-treated samples...
In a previously reported double-blind, randomized controlled trial (RCT), we demonstrated that daily supplementation with anserine (750 mg) and carnosine (250 improves brain blood flow memory function in elderly people. Here, conducted sub-analysis of MRI data test scores from the same RCT to determine whether anserine/carnosine specifically benefits people carrying APOE e4 allele, which is risk gene for accelerated aging onset Alzheimer's Disease. We collected 68 participants aged 65 years...
The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST Bombyx mori (bmGSTO) was shown to be induced after exposure environmental stresses. A soluble form recombinant protein (rbmGSTO) functionally overexpressed Escherichia coli cells and purified homogeneity. Cys 38 Pro 39 were found highly conserved GSTs, their roles investigated by site-directed mutagenesis/kinetic analysis. Mutations...
Piceatannol is a phenolic stilbenoid and metabolite of resveratrol which found in red wine. (PIC) commonly exhibits anti-inflammatory, antiplatelet antiproliferative activity. In the present study, anti-allergic anti-inflammatory mechanisms PIC were investigated by examining effects on pro‑inflammatory cytokine release phosphorylation mitogen-activated protein (MAP) kinases (ERK, JNK p38) human mast cell line. dose-dependently inhibited compound 48/80-induced systemic anaphylaxis...
Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With focus on neurodegenerative conditions such Alzheimer's disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for first time from A....
In our previous studies, we reported that SIRT1 prevents cellular senescence in human fibroblast, and SIRT1-induced inhibition of is due to enhanced hTERT gene expression. this study, investigate the molecular mechanisms behind potentiation transcription show FOXO3a functions downstream induction by enhancing Furthermore, found FOXO3a-induced expression regulated a c-MYC/E-box dependent manner. addition, binds novel binding element c-MYC promoter, interaction activates gene. The resulting...
Pomegranate‐derived polyphenols are expected to prevent life‐style related diseases. In this study, we evaluated the ability of 8 pomegranate‐derived polyphenols, along with other augment SIRT3 , a mammalian SIR2 homolog localized in mitochondria. We established system for screening foods/food ingredients that promoter Caco‐2 cells and identified 3 SIRT3‐augmenting (eucalbanin B, pomegraniin A, eucarpanin T 1 ). Among them, A activated superoxide dismutase 2 (SOD2) through SIRT3‐mediated...
One of the conventional strategies for treating osteoporosis is to eliminate multinucleated osteoclasts that are responsible bone resorption. Our previous study revealed ursolic acid, isolated from leaves loquat used as tasty tea in Japan, suppressed osteoclastogenesis. We confirmed acid exhibited osteoclast differentiation inhibitory activity with an 50% concentration (IC50) value 5.4 ± 0.96 μM. To disclose its mechanism action, this first uses polymer-coated magnetic nanobeads identify...
Although thinning hair and alopecia are not recognized as severe diseases, loss has implications for mental health quality of life; therefore, a large number studies have been carried out to develop novel growth agents. In the present study, we aimed examine potential telomerase reverse transcriptase (TERT), because TERT overexpression in skin activates resting follicle bulge stem cells, which triggers initiation new phase promotes synthesis. To this end, screened polyphenols that activate...
We investigated whether any local moieties within a protein molecule could completely refold from the denatured state to regain native conformation. Bovine beta-lactoglobulin (beta-LG) was in presence of guanidine hydrochloride (GdnHCl) as denaturant. Renaturation beta-LG attempted by dialyzing remove GdnHCl. The renatured molecules regained same retinol binding activity that beta-LG, and physicochemical studies also indicated refolding had been almost successful. Local structural...
Abstract Carnosine (β-Ala-l-His), an imidazole dipeptide, is known to have many functions. Recently, we demonstrated in a double-blind randomized controlled trial that carnosine capable of preserving cognitive function elderly people. In the current study, assessed ability activate brain, and tried clarify molecular mechanisms behind this activation. Our results demonstrate permeates blood brain barrier activates glial cells within causing them secrete neurotrophins, including BDNF NGF....
Recently, we showed that imidazole dipeptide such as carnosine contained abundantly in chicken breast meat improves brain function a double-blind randomized controlled trial. However, the underlying molecular mechanisms remain unknown. Here, investigated whether activates intestinal epithelial cells and induces secretion of factors activate function. We focused on exosomes derived from mediators brain-gut interaction. Results Caco-2 treated with significantly induced neurite growth SH-SY5Y...